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公开(公告)号:US08901289B2
公开(公告)日:2014-12-02
申请号:US12775843
申请日:2010-05-07
申请人: Kyeong-Eun Jung , Alexei Kayushin , Moon Hee Kim , Kyung-Il Kim , Sungwon Kim , Yumi Ji
发明人: Kyeong-Eun Jung , Alexei Kayushin , Moon Hee Kim , Kyung-Il Kim , Sungwon Kim , Yumi Ji
IPC分类号: C07H21/02 , C07H19/167 , C07H19/067 , C07H19/10 , C07H19/20
CPC分类号: C07H21/02 , C07H1/00 , C07H19/067 , C07H19/10 , C07H19/167 , C07H19/20
摘要: The present invention provides a method for preparing nucleotide oligomers, including (a) coupling a nucleotide dimer or nucleotide trimer to a nucleoside attached to solid supports or to universal solid supports as a starting material; (b) sequentially coupling nucleotide monomers to the resulting structures of Step (a) to prepare a nucleotide oligomer; and (c) removing the nucleotide oligomers from the solid supports.The method of the present invention provides nucleotide oligomers having 15-20% higher purity than the conventional art. The present invention enables the efficient and inexpensive synthesis of nucleotide oligomers with high purity within a shorter period of time.
摘要翻译: 本发明提供了制备核苷酸寡聚体的方法,包括(a)将核苷酸二聚体或核苷酸三聚体与连接至固体支持物的核苷或作为起始材料的通用固体支持物偶联; (b)顺序地将核苷酸单体与步骤(a)的所得结构偶联以制备核苷酸寡聚物; 和(c)从固体支持物中除去核苷酸寡聚体。 本发明的方法提供了具有比常规技术高15-20%的核苷酸寡聚物。 本发明能够在更短的时间内高效且廉价地合成具有高纯度的核苷酸低聚物。
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公开(公告)号:US08846630B2
公开(公告)日:2014-09-30
申请号:US13512583
申请日:2010-11-01
申请人: Misun Won , Kyung-Sook Chung , Young Joo Kim , Hye Kyung Hong , Young Il Yeom , Chae Ok Yun , Yu-Kyoung Oh , Kyung Bin Song , Hee Gu Lee , Young Ho Kim , Moon Hee Kim , Kyeong-Eun Jung , Seok Hoon Song
发明人: Misun Won , Kyung-Sook Chung , Young Joo Kim , Hye Kyung Hong , Young Il Yeom , Chae Ok Yun , Yu-Kyoung Oh , Kyung Bin Song , Hee Gu Lee , Eun Young Song , Young Ho Kim , Moon Hee Kim , Kyeong-Eun Jung
IPC分类号: C12N15/11 , C07H21/04 , A61K31/711 , A61K31/713 , A61K31/7105
CPC分类号: C12N15/113 , A61K31/7105 , A61K31/711 , A61K31/713 , C12N2310/531
摘要: The present invention provides a pharmaceutical composition for treating cancer, comprising at least one selected from deoxyribonucleic acids (DNA) for encoding small interfering RNA (siRNA) which complementarily binds to the base sequence of the transcript (mRNA transcript) of the FLJ25416 gene, represented by sequence number 3, sequence number 5, and sequence number 7 to inhibit the intracellular expression of the FLJ25416 gene, antisense RNA which inhibits expression of the FLJ25416 gene, and short hairpin RNA (shRNA) which inhibits expression of the FLJ25416 gene. As the siRNA, which is complementary to the base sequence of the transcript (mRNA transcript) of the FLJ25416 gene, the antisense RNA, and the shRNA, according to the present invention, inhibit expression of the FLJ25416 gene which is known to be expressed in cancer cells, and thus kill cancer cells, the composition of the present invention can be used as a novel anti-cancer agent.
摘要翻译: 本发明提供了一种用于治疗癌症的药物组合物,其包含选自用于编码与FLJ25416基因的转录物(mRNA转录物)的碱基序列互补结合的小干扰RNA(siRNA)的脱氧核糖核酸(DNA)中的至少一种,代表 通过序列号3,序列号5和序列号7来抑制FLJ25416基因的细胞内表达,抑制FLJ25416基因表达的反义RNA和抑制FLJ25416基因表达的短发夹RNA(shRNA)。 作为与根据本发明的FLJ25416基因,反义RNA和shRNA的转录物(mRNA转录物)的碱基序列互补的siRNA抑制FLJ25416基因的表达,已知其表达于 癌细胞,因此杀死癌细胞,本发明的组合物可以用作新的抗癌剂。
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公开(公告)号:US20130028957A1
公开(公告)日:2013-01-31
申请号:US13512583
申请日:2010-11-01
申请人: Misun Won , Kyung-Sook Chung , Young Joo Kim , Hye Kyung Hong , Young Il Yeom , Chae Ok Yun , Yu-Kyoung Oh , Kyung Bin Song , Hee Gu Lee , Young Ho Kim , Moon Hee Kim , Kyeong-Eun Jung , Seok Hoon Song
发明人: Misun Won , Kyung-Sook Chung , Young Joo Kim , Hye Kyung Hong , Young Il Yeom , Chae Ok Yun , Yu-Kyoung Oh , Kyung Bin Song , Hee Gu Lee , Eun Young Song , Young Ho Kim , Moon Hee Kim , Kyeong-Eun Jung
IPC分类号: A61K31/7105 , C07H21/04 , A61P35/00 , A61K31/711 , A61K9/127 , C07H21/02 , A61K31/713
CPC分类号: C12N15/113 , A61K31/7105 , A61K31/711 , A61K31/713 , C12N2310/531
摘要: The present invention provides a pharmaceutical composition for treating cancer, comprising at least one selected from deoxyribonucleic acids (DNA) for encoding small interfering RNA (siRNA) which complementarily binds to the base sequence of the transcript (mRNA transcript) of the FLJ25416 gene, represented by sequence number 3, sequence number 5, and sequence number 7 to inhibit the intracellular expression of the FLJ25416 gene, antisense RNA which inhibits expression of the FLJ25416 gene, and short hairpin RNA (shRNA) which inhibits expression of the FLJ25416 gene. As the siRNA, which is complementary to the base sequence of the transcript (mRNA transcript) of the FLJ25416 gene, the antisense RNA, and the shRNA, according to the present invention, inhibit expression of the FLJ25416 gene which is known to be expressed in cancer cells, and thus kill cancer cells, the composition of the present invention can be used as a novel anti-cancer agent.
摘要翻译: 本发明提供了一种用于治疗癌症的药物组合物,其包含选自用于编码与FLJ25416基因的转录物(mRNA转录物)的碱基序列互补结合的小干扰RNA(siRNA)的脱氧核糖核酸(DNA)中的至少一种,代表 通过序列号3,序列号5和序列号7来抑制FLJ25416基因的细胞内表达,抑制FLJ25416基因表达的反义RNA和抑制FLJ25416基因表达的短发夹RNA(shRNA)。 作为与根据本发明的FLJ25416基因,反义RNA和shRNA的转录物(mRNA转录物)的碱基序列互补的siRNA抑制FLJ25416基因的表达,已知其表达于 癌细胞,因此杀死癌细胞,本发明的组合物可用作新的抗癌剂。
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公开(公告)号:US20100273999A1
公开(公告)日:2010-10-28
申请号:US12775843
申请日:2010-05-07
申请人: Kyeong-Eun Jung , Alexei Kayushin , Moon Hee Kim , Kyung-Il Kim , Sungwon Kim , Yumi Ji
发明人: Kyeong-Eun Jung , Alexei Kayushin , Moon Hee Kim , Kyung-Il Kim , Sungwon Kim , Yumi Ji
CPC分类号: C07H21/02 , C07H1/00 , C07H19/067 , C07H19/10 , C07H19/167 , C07H19/20
摘要: The present invention provides a method for preparing nucleotide oligomers, including (a) coupling a nucleotide dimer or nucleotide trimer to a nucleoside attached to solid supports or to universal solid supports as a starting material; (b) sequentially coupling nucleotide monomers to the resulting structures of Step (a) to prepare a nucleotide oligomer; and (c) removing the nucleotide oligomers from the solid supports.The method of the present invention provides nucleotide oligomers having 15-20% higher purity than the conventional art. The present invention enables the efficient and inexpensive synthesis of nucleotide oligomers with high purity within a shorter period of time.
摘要翻译: 本发明提供了制备核苷酸寡聚体的方法,包括(a)将核苷酸二聚体或核苷酸三聚体与连接至固体支持物的核苷或作为起始材料的通用固体支持物偶联; (b)顺序地将核苷酸单体与步骤(a)的所得结构偶联以制备核苷酸寡聚物; 和(c)从固体支持物中除去核苷酸寡聚体。 本发明的方法提供了具有比常规技术高15-20%的核苷酸寡聚物。 本发明能够在更短的时间内高效且廉价地合成具有高纯度的核苷酸低聚物。
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公开(公告)号:US08486905B2
公开(公告)日:2013-07-16
申请号:US12746722
申请日:2008-12-05
申请人: Misun Won , Kyung-Sook Chung , Young-Joo Kim , Shin-Jung Choi , Young Il Yeom , Seon-Young Kim , Kyung Bin Song , Hee Gu Lee , Eun Young Song , Young Ho Kim , Ho Kyung Chun , Chae-Ok Yun , Moon Hee Kim , Kyeong-Eun Jung , Sun-Jung Cho
发明人: Misun Won , Kyung-Sook Chung , Young-Joo Kim , Shin-Jung Choi , Young Il Yeom , Seon-Young Kim , Kyung Bin Song , Hee Gu Lee , Eun Young Song , Young Ho Kim , Ho Kyung Chun , Chae-Ok Yun , Moon Hee Kim , Kyeong-Eun Jung , Sun-Jung Cho
CPC分类号: G01N33/57484 , A61K31/7088 , A61K38/1709 , C12N15/1135 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2710/10343 , C12Q1/6886 , C12Q2600/136 , C12Q2600/158 , G01N2500/00 , G01N2500/04
摘要: Provided are a composition for diagnosing cancer or screening an anticancer drug comprising an FLJ25416 gene or an antibody directed to a protein expressed from the FLJ25416 gene, a composition for treating cancer comprising an inhibitor of the gene or an inhibitor of the protein expressed from the gene and a pharmaceutically acceptable carrier, and a kit for diagnosing cancer comprising at least one of the FLJ25416 gene and the protein expressed from the FLJ25416 gene. The FLJ25416 gene is expressed at high level in specific cancer cells, and induces an increase in proliferation rate of normal cells. The expression of the gene is suppressed, which results in an inhibitory effect on cancer cell growth. Thus, the FLJ25416 gene can be used as a target gene for diagnosis or treatment of cancer.
摘要翻译: 提供了用于诊断癌症或筛选包含FLJ25416基因或针对从FLJ25416基因表达的蛋白质的抗体的抗癌药物的组合物,用于治疗癌症的组合物,其包含该基因的抑制剂或从该基因表达的蛋白质的抑制剂 和药学上可接受的载体,以及用于诊断癌症的试剂盒,其包含FLJ25416基因和从FLJ25416基因表达的蛋白质中的至少一种。 FLJ25416基因在特异性癌细胞中表达量高,诱导正常细胞增殖。 该基因的表达被抑制,这导致对癌细胞生长的抑制作用。 因此,FLJ25416基因可用作癌症诊断或治疗的靶基因。
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公开(公告)号:US20110008370A1
公开(公告)日:2011-01-13
申请号:US12746722
申请日:2008-12-05
申请人: Misun Won , Kyung-Sook Chung , Young-Joo Kim , Shin-Jung Choi , Young Il Yeom , Seon-Young Kim , Kyung Bin Song , Hee Gu Lee , Eun Young Song , Young Ho Kim , Ho Kyung Chun , Chae-Ok Yun , Moon Hee Kim , Kyeong-Eun Jung , Sun-Jung Cho
发明人: Misun Won , Kyung-Sook Chung , Young-Joo Kim , Shin-Jung Choi , Young Il Yeom , Seon-Young Kim , Kyung Bin Song , Hee Gu Lee , Eun Young Song , Young Ho Kim , Ho Kyung Chun , Chae-Ok Yun , Moon Hee Kim , Kyeong-Eun Jung , Sun-Jung Cho
IPC分类号: A61K39/395 , C07H21/02 , A61K31/713 , C07H21/00 , C07K16/18 , C07K14/47 , C12Q1/68 , G01N33/574 , A61P35/00
CPC分类号: G01N33/57484 , A61K31/7088 , A61K38/1709 , C12N15/1135 , C12N15/86 , C12N2310/111 , C12N2310/14 , C12N2710/10343 , C12Q1/6886 , C12Q2600/136 , C12Q2600/158 , G01N2500/00 , G01N2500/04
摘要: Provided are a composition for diagnosing cancer or screening an anticancer drug comprising an FLJ25416 gene or an antibody directed to a protein expressed from the FLJ25416 gene, a composition for treating cancer comprising an inhibitor of the gene or an inhibitor of the protein expressed from the gene and a pharmaceutically acceptable carrier, and a kit for diagnosing cancer comprising at least one of the FLJ25416 gene and the protein expressed from the FLJ25416 gene. The FLJ25416 gene is expressed at high level in specific cancer cells, and induces an increase in proliferation rate of normal cells. The expression of the gene is suppressed, which results in an inhibitory effect on cancer cell growth. Thus, the FLJ25416 gene can be used as a target gene for diagnosis or treatment of cancer.
摘要翻译: 提供了用于诊断癌症或筛选包含FLJ25416基因或针对由FLJ25416基因表达的蛋白质的抗体的抗癌药物的组合物,用于治疗癌症的组合物,其包含该基因的抑制剂或从该基因表达的蛋白质的抑制剂 和药学上可接受的载体,以及用于诊断癌症的试剂盒,其包含FLJ25416基因和从FLJ25416基因表达的蛋白质中的至少一种。 FLJ25416基因在特异性癌细胞中表达量高,诱导正常细胞增殖。 该基因的表达被抑制,这导致对癌细胞生长的抑制作用。 因此,FLJ25416基因可用作癌症诊断或治疗的靶基因。
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