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公开(公告)号:US10359425B2
公开(公告)日:2019-07-23
申请号:US12556480
申请日:2009-09-09
申请人: Larry Gold , Marty Stanton , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A. E. Stewart
发明人: Larry Gold , Marty Stanton , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A. E. Stewart
IPC分类号: G01N33/574
摘要: The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of lung cancer. In one aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose lung cancer or permit the differential diagnosis of pulmonary nodules as benign or malignant. In another aspect, methods are provided for diagnosing lung cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 1, Col. 2, wherein the individual is classified as having lung cancer, or the likelihood of the individual having lung cancer is determined, based on the at least one biomarker value.
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公开(公告)号:US08703416B2
公开(公告)日:2014-04-22
申请号:US13525271
申请日:2012-06-15
申请人: Glenn Sanders , Evaldas Katilius , Larry Gold
发明人: Glenn Sanders , Evaldas Katilius , Larry Gold
CPC分类号: C12N15/115 , C12N5/061 , C12N2310/16 , C12N2320/10 , C12N2330/00
摘要: The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity and specificity to capture and immobilize intact sperm cells in the presence of female epithelial cells and other non-sperm semen components. The present disclosure also describes affinity-based methods for the detection of sperm cells in samples, including from forensic sample surrogates consisting of swab eluates containing a mixture of HeLa cells and sperm cells. The present disclosure describes methods for eluting sperm cell samples from swabs; methods for purifying sperm cells and methods for amplification and analysis of male DNA. The affinity-based system described herein is inexpensive, simple to use and easily implemented in forensic laboratories.
摘要翻译: 本公开描述了具有足够高的亲和性和特异性的适体的分离,鉴定和纯化,以在存在雌性上皮细胞和其它非精液精液组分的情况下捕获和固定完整精子细胞。 本公开还描述了用于检测样品中精子细胞的基于亲和性的方法,包括由包含HeLa细胞和精子细胞的混合物的拭子洗脱液组成的法医样品替代物。 本公开描述了从拭子洗脱精子细胞样品的方法; 纯化精子细胞的方法和扩增和分析雄性DNA的方法。 本文描述的基于亲和性的系统是廉价的,易于使用并且容易地在法医实验室中实施。
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公开(公告)号:US20120165217A1
公开(公告)日:2012-06-28
申请号:US13391794
申请日:2010-04-03
申请人: Larry Gold , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart , Michael Riel-Mehan , Mark Messenbaugh , Randee S. Schwartz , Jeffery Walker , Stephen Alaric Williams , Malti Nikrad
发明人: Larry Gold , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart , Michael Riel-Mehan , Mark Messenbaugh , Randee S. Schwartz , Jeffery Walker , Stephen Alaric Williams , Malti Nikrad
CPC分类号: G01N33/57449 , G16B40/00
摘要: The present disclosure includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of cancer. In one aspect, the disclosure provides biomarkers that can be used alone or in various combinations to diagnose cancer. In another aspect, methods are provided for diagnosing cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 24, wherein the individual is classified as having cancer, or the likelihood of the individual having cancer is determined, based on the at least one biomarker value.
摘要翻译: 本公开包括用于癌症的检测和诊断的生物标志物,方法,装置,试剂,系统和试剂盒。 一方面,本公开提供可以单独使用或以各种组合使用以诊断癌症的生物标志物。 在另一方面,提供了用于诊断个体癌症的方法,其中所述方法包括在来自个体的生物样品中检测至少一种对应于选自表24中提供的生物标志物组的生物标志物的生物标记值, 其中所述个体基于所述至少一种生物标志物值被分类为具有癌症或确定个体患有癌症的可能性。
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公开(公告)号:US20120115752A1
公开(公告)日:2012-05-10
申请号:US13382493
申请日:2010-07-09
申请人: Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold , Thale C. Jarvis , Jeffrey D. Carter
发明人: Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold , Thale C. Jarvis , Jeffrey D. Carter
CPC分类号: C12N15/1048 , C12N15/115 , C12N2310/16 , C12Q1/6811 , G01N33/5308 , C12Q2525/117 , C12Q2525/205 , C12Q2541/101
摘要: The present disclosure describes the identification and use of aptamers and photoaptamers having slower dissociation rate constants than those obtained using previously described methods. Specifically, the present disclosure describes methods for the identification and use of aptamers to one or more targets within a histological or cytological sample, which have slow rates of dissociation. The aptamers may be used to assess localization, relative density, and presence or absence of one or more targets in cytological and histological samples. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. The aptamers may also be used to introduce target specific signal moieties. In addition to target identification, the aptamers may be used to amplify signal generation through a variety of methods.
摘要翻译: 本公开描述了具有比使用先前描述的方法获得的那些更快的解离速率常数的适配体和光致抗体的鉴定和使用。 具体地,本公开描述了用于鉴定和使用适配体到组织学或细胞学样品中的一种或多种目标的方法,其具有缓慢的解离速率。 适配体可用于评估细胞学和组织学样品中的一种或多种靶标的定位,相对密度和存在或不存在。 可以选择对于样品收集的给定疾病状态的特异性和诊断性的靶标。 适体也可以用于引入目标特异性信号部分。 除目标识别之外,适体可用于通过各种方法放大信号产生。
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公开(公告)号:US20110212030A1
公开(公告)日:2011-09-01
申请号:US12624314
申请日:2009-11-23
申请人: Larry Gold , Paul Schmidt , Nebojsa Janjic
发明人: Larry Gold , Paul Schmidt , Nebojsa Janjic
IPC分类号: A61K49/10
CPC分类号: A61K9/1271 , A61K9/1272 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N9/1276 , C12N15/1048 , C12N15/115 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12Q1/37 , C12Q1/6811 , C40B40/00 , F02B2075/027 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , G01N2333/163 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96455 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976 , C12Q2541/101 , C12Q2563/161
摘要: This invention discloses a method for preparing a therapeutic or diagnostic complex comprised of a nucleic acid ligand and a lipophilic compound or non-immunogenic, high molecular weight compound by identifying a nucleic acid ligand by SELEX methodology and associating the nucleic acid ligand with a lipophilic compound or a non-immunogenic, high molecular weight compound. The invention further discloses complexes comprising one or more nucleic acid ligands in association with a lipophilic compound or non-immunogenic, high molecular weight compound.
摘要翻译: 本发明公开了一种由核酸配体和亲脂性化合物或非免疫原性高分子量化合物组成的治疗或诊断复合物的方法,其通过使用SELEX方法鉴定核酸配体并将核酸配体与亲脂性化合物 或非免疫原性的高分子量化合物。 本发明进一步公开了包含与亲脂性化合物或非免疫原性高分子量化合物结合的一种或多种核酸配体的复合物。
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公开(公告)号:US20100209935A1
公开(公告)日:2010-08-19
申请号:US12771895
申请日:2010-04-30
申请人: Larry Gold , Dominic Zichi
发明人: Larry Gold , Dominic Zichi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要: A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.
摘要翻译: 公开了一种核酸配体“生物芯片”,其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。 每个核酸配体特异性地并且强烈地结合到测试混合物(例如体液)中所含的特定靶分子。 靶分子包括但不限于蛋白质(细胞,病毒,细菌等)激素,糖,代谢副产物,辅因子和中间体,药物和毒素。 将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。 然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。 然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。
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公开(公告)号:US07709192B2
公开(公告)日:2010-05-04
申请号:US10375487
申请日:2003-02-27
申请人: Larry Gold , Dominic Zichi
发明人: Larry Gold , Dominic Zichi
CPC分类号: C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要: A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.
摘要翻译: 公开了一种核酸配体“生物芯片”,其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。 每个核酸配体特异性地并且强烈地结合到测试混合物(例如体液)中所含的特定靶分子。 靶分子包括但不限于蛋白质(细胞,病毒,细菌等)激素,糖,代谢副产物,辅因子和中间体,药物和毒素。 将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。 然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。 然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。
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公开(公告)号:US20100070191A1
公开(公告)日:2010-03-18
申请号:US12556480
申请日:2009-09-09
申请人: Larry Gold , Marty Stanton , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart
发明人: Larry Gold , Marty Stanton , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart
IPC分类号: G01N33/574 , C12Q1/02 , G06F19/00
CPC分类号: G01N33/57423
摘要: The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of lung cancer. In one aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose lung cancer or permit the differential diagnosis of pulmonary nodules as benign or malignant. In another aspect, methods are provided for diagnosing lung cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 1, Col. 2, wherein the individual is classified as having lung cancer, or the likelihood of the individual having lung cancer is determined, based on the at least one biomarker value.
摘要翻译: 本申请包括用于肺癌的检测和诊断的生物标志物,方法,装置,试剂,系统和试剂盒。 在一个方面,该应用提供可以单独使用或以各种组合使用以诊断肺癌或允许肺结节的鉴别诊断为良性或恶性的生物标志物。 在另一方面,提供了用于诊断个体中的肺癌的方法,其中所述方法包括在来自个体的生物样品中检测至少一种对应于选自表1中提供的生物标志物组的至少一种生物标志物的生物标记值 ,第2栏,其中基于所述至少一种生物标记值,将个体分类为具有肺癌或确定患有肺癌的个体的可能性。
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公开(公告)号:US20090118481A1
公开(公告)日:2009-05-07
申请号:US12133132
申请日:2008-06-04
申请人: David H. Parma , Brian Hicke , Philippe Bridonneau , Larry Gold
发明人: David H. Parma , Brian Hicke , Philippe Bridonneau , Larry Gold
IPC分类号: C07H21/00
CPC分类号: C07H19/10 , C07H19/06 , C07H21/00 , C07K14/001 , C12N9/1276 , C12N15/1048 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12Q1/37 , C12Q1/6804 , C12Q1/6811 , C12Q1/70 , C40B40/00 , F02B2075/027 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , G01N2333/163 , G01N2333/4724 , G01N2333/7056 , G01N2333/8125 , G01N2333/96433 , G01N2333/966 , G01N2333/974 , C12Q2525/101
摘要: This invention discloses high-affinity oligonucleotide ligands to lectins, specifically nucleic acid ligands having the ability to bind to the lectins, wheat germ agglutinin, L-selectin, E-selectin and P-selectin. Also disclosed are the methods for obtaining such ligands. This invention discloses high-affinity oligonucleotide ligands to lectins, specifically nucleic acid ligands having the ability to bind to the lectins, wheat germ agglutinin, L-selectin, E-selectin and P-selectin. Also disclosed are the methods for obtaining such ligands.
摘要翻译: 本发明公开了具有凝集素的高亲和力寡核苷酸配体,特别是具有与凝集素结合的能力的核酸配体,小麦胚芽凝集素,L-选择蛋白,E-选择蛋白和P-选择蛋白。 还公开了获得这种配体的方法。 本发明公开了具有凝集素的高亲和力寡核苷酸配体,特别是具有与凝集素结合的能力的核酸配体,小麦胚芽凝集素,L-选择蛋白,E-选择蛋白和P-选择蛋白。 还公开了获得这种配体的方法。
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公开(公告)号:US20090098549A1
公开(公告)日:2009-04-16
申请号:US12175388
申请日:2008-07-17
申请人: Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
发明人: Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6811 , C12N15/1048 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12Q2541/101 , C12Q2525/205 , C12Q2525/117
摘要: The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.
摘要翻译: 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。 本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。
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