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公开(公告)号：US20130090470A1
公开(公告)日：2013-04-11
申请号：US13614948
申请日：2012-09-13
申请人： Schuyler CORRY , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler CORRY , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C07D503/00 , C07F5/02 , C07D501/58
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，还公开了分析样品中存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。
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2.发明申请公开(公告)号：US20120077211A1
公开(公告)日：2012-03-29
申请号：US13139873
申请日：2009-12-15
IPC分类号： G01N33/53 , C12Q1/28 , C09K3/00 , C07C211/54
CPC分类号： G01N33/532 , C01B15/037
摘要： Compositions, assays, methods, and kits are disclosed for use in applications that utilize oxidation of a chromogenic electron donor such as diaminobenzidine (DAB) to generate a signal. Applications include, but are not limited to, immunohistochemistry, chromogenic in situ hybridization, Western blots, Northern blots, Southern blots, ELISA assays, and microarray detection. The compositions, assays, methods, and kits disclosed herein make use of a novel, stabilized formulation of DAB and a novel, stabilized formulation of hydrogen peroxide.
摘要翻译： 公开了组合物，测定法，方法和试剂盒，用于利用显色电子给体如二氨基联苯胺（DAB）氧化产生信号的应用中。 应用包括但不限于免疫组化，显色原位杂交，Western印迹，Northern印迹，Southern印迹，ELISA测定和微阵列检测。 本文公开的组合物，测定方法和试剂盒使用DAB的新型稳定制剂和新型稳定的过氧化氢制剂。
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公开(公告)号：US07208271B2
公开(公告)日：2007-04-24
申请号：US10306347
申请日：2002-11-27
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
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4.发明授权Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 失效来自Thermus物种Z05的突变的热稳定性核酸聚合酶
公开(公告)号：US5455170A
公开(公告)日：1995-10-03
申请号：US113531
申请日：1993-08-27
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/703
摘要： A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
摘要翻译： 纯化的热稳定酶源自嗜热菌菌种Z05。 该酶具有DNA聚合酶，活性逆转录酶活性和任选的5'→3'核酸外切酶活性。 酶可以是天然的或重组的，并且可以在温度循环链反应中与引物和核苷三磷酸一起使用，其中至少一个核酸序列以现有序列的量被扩增。
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公开(公告)号：US08865891B2
公开(公告)日：2014-10-21
申请号：US13614948
申请日：2012-09-13
申请人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C07D501/36 , C07D501/58 , C07D501/00 , C07F5/02 , C07D503/00 , C07D499/00 , C12Q1/34
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactamase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactamase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactamase or instructions for using the components in an assay.
摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品中β-内酰胺酶的存在的方法，例如，作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。
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公开(公告)号：US20140193841A1
公开(公告)日：2014-07-10
申请号：US14124354
申请日：2012-06-07
CPC分类号： G01N33/525 , B82Y15/00 , C09K11/08 , G01N33/581 , G01N33/588
摘要： Fluorogenic semiconductor nanocrystals and compositions thereof are provided herein, including kits, assay systems and methods for their preparation and use.
摘要翻译： 本文提供了荧光半导体纳米晶体及其组合物，包括其制备和使用的试剂盒，测定系统和方法。
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公开(公告)号：US08507198B2
公开(公告)日：2013-08-13
申请号：US12471292
申请日：2009-05-22
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
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公开(公告)号：US20100021905A1
公开(公告)日：2010-01-28
申请号：US12471292
申请日：2009-05-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
摘要翻译： 本发明涉及用于分离和/或鉴定核酸的方法。 本发明还提供用于分离和/或鉴定核酸的试剂盒。
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9.发明授权Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases 有权使用表面活性剂和蛋白酶分离核酸的组合物，方法和试剂盒公开(公告)号：US07303876B2
公开(公告)日：2007-12-04
申请号：US10800137
申请日：2004-03-11
CPC分类号： C12N15/1006
摘要： The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples.
摘要翻译： 本发明涉及用于从包括整个组织的生物样品中分离核酸的组合物和方法。 本发明还提供用于从生物样品中分离核酸的试剂盒。
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公开(公告)号：US20070224630A1
公开(公告)日：2007-09-27
申请号：US11789352
申请日：2007-04-23
申请人： Douglas Bost , Lawrence Greenfield
发明人： Douglas Bost , Lawrence Greenfield
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
摘要翻译： 本发明涉及用于分离和/或鉴定核酸的方法。 本发明还提供用于分离和/或鉴定核酸的试剂盒。
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