公开(公告)号：US20050279636A1

公开(公告)日：2005-12-22

申请号：US11210767

申请日：2005-08-25

申请人： Masahiko Yoneta ,  Yohei Sasaki ,  Makiko Kawaguchi

发明人： Masahiko Yoneta ,  Yohei Sasaki ,  Makiko Kawaguchi

IPC分类号： G01N27/447 ,  G01N27/27 ,  G01N27/453

CPC分类号： G01N27/44747

摘要： The present invention herein provides a gel for electrophoresis which comprises a gel prepared from single-stranded glucan which has β-1,3-glucoside bonds as a main chain and β-1,6-glucoside bonds on side chains, or a mixture of the glucan with a second polysaccharide, or acrylamide and/or derivative thereof; and a method for the preparation of a gel for electrophoresis comprising the steps of dissolving a glucan, which has β-1,3-glucoside bonds as a main chain and β-1,6-glucoside bonds on side chains, in a first solvent which can dissociate the helical structure of the glucan to thus form single-stranded glucan; removing the first solvent; dissolving the single-stranded glucan in a second solvent capable of dissolving the same; and then heat-treating the resulting solution. The pore size of the gel according to the present invention can easily be controlled, the gel permits the isolation and/or analysis of a nucleic acid or a protein whose molecular weight falls within a wider range and the gel never interferes with the detection, through the staining technique, of such a nucleic acid or a protein separated and developed on or within the gel.

摘要翻译： 本发明提供了一种电泳用凝胶，其包含由单链葡聚糖制成的凝胶，其具有作为主链的β-1,3-葡萄糖苷键和侧链上的β-1,6-糖苷键，或 具有第二多糖的葡聚糖或丙烯酰胺和/或其衍生物; 以及制备电泳用凝胶的方法，包括以下步骤：在第一溶剂中溶解具有β-1,3-葡糖苷键作为主链的葡聚糖和β-1,6-糖苷键在侧链上 其可以解离葡聚糖的螺旋结构从而形成单链葡聚糖; 去除第一溶剂; 将单链葡聚糖溶解在能溶解该溶液的第二溶剂中; 然后对所得溶液进行热处理。 根据本发明的凝胶的孔径可以容易地控制，凝胶允许分离和/或分析分子量在更宽范围内的核酸或蛋白质，并且凝胶不会干扰检测，通过 这种核酸或在凝胶上或内部分离和显影的蛋白质的染色技术。