公开(公告)号：US09493827B2

公开(公告)日：2016-11-15

申请号：US13522225

申请日：2011-01-13

Applicant: Christof Von Kalle ,  Manfred Schmidt ,  Richard Gabriel ,  Ali Nowrouzi ,  Anne Arens ,  Angelo Lombardo ,  Luigi Naldini

Inventor： Christof Von Kalle ,  Manfred Schmidt ,  Richard Gabriel ,  Ali Nowrouzi ,  Anne Arens ,  Angelo Lombardo ,  Luigi Naldini

IPC: C12Q1/68 ,  C12Q1/70 ,  C12N15/90

CPC classification number: C12Q1/686 ,  C12N15/907 ,  C12N2740/15021 ,  C12N2740/15031 ,  C12N2740/15045 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/70 ,  C12Q2521/301

Abstract: The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease.

Abstract translation: 本发明涉及用于确定宿主细胞中双链断裂的体内定位的方法，包括孵育疑似包含DNA双链断裂的宿主细胞和包含已知序列的线性多核苷酸，检测体内插入 所述多核苷酸在所述宿主细胞的基因组中的位点，以及评估双链断裂的体内定位。 本发明进一步设想的是获得具有改变的体内特异性的核酸内切酶的方法。 最后，本发明涉及用于确定内切核酸酶的体内特异性的试剂盒。

公开(公告)号：US20130109830A1

公开(公告)日：2013-05-02

申请号：US13696448

申请日：2011-05-02

Applicant: Jens Krause ,  Manfred Schmidt

Inventor： Jens Krause ,  Manfred Schmidt

IPC: C08G18/76

CPC classification number: C08G18/7692 ,  C08G18/022 ,  C08G18/089 ,  C08G18/10 ,  C08G18/18 ,  C08G18/794 ,  C08G18/3206

Abstract: The invention relates to cellular and non-cellular polyurethane (PUR) elastomers made of NCO functional prepolymers based on polyisocyanates having portions of non-monomers, to a method for producing same, and to the use thereof.

Abstract translation: 本发明涉及由基于具有非单体部分的多异氰酸酯的NCO官能预聚物制备的细胞和非细胞聚氨酯（PUR）弹性体，其制备方法及其用途。

公开(公告)号：US20120252071A1

公开(公告)日：2012-10-04

申请号：US13436110

申请日：2012-03-30

Applicant: Michael Greif ,  Christian Rudolph ,  Manfred Schmidt ,  Harald Sobek ,  Johann-Peter Thalhofer

Inventor： Michael Greif ,  Christian Rudolph ,  Manfred Schmidt ,  Harald Sobek ,  Johann-Peter Thalhofer

IPC: C12P19/34 ,  C12N9/12

CPC classification number: C12N9/1247 ,  C12Y207/07006

Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.

Abstract translation: 本公开提供了T7RNA聚合酶的新变体。 根据本发明的T7变体的实施方案包括T7多肽的氨基酸序列的位置723上的半胱氨酸 - 丝氨酸取代。 根据本发明的T7变体的实施方案具有DNA依赖性RNA聚合酶酶活性，并且通过氧化硫醇基形成分子内同型二聚体的趋势降低。 本文公开的T7变体内的氨基酸取代最小程度地影响T7多肽的RNA聚合酶活性。 此外，所公开的实施方案的突变可以任选地与与野生型参考物相比提供增强的热稳定性的突变组合。

公开(公告)号：US08177859B2

公开(公告)日：2012-05-15

申请号：US10494581

申请日：2002-09-20

Applicant: Manfred Schmidt ,  Stephanie Schauhoff ,  Frank Gähr

Inventor： Manfred Schmidt ,  Stephanie Schauhoff ,  Frank Gähr

IPC: D06M13/322 ,  D06M11/38 ,  C09K21/00

CPC classification number: D06M13/358 ,  D06M13/292 ,  D06M13/364 ,  D06M13/447 ,  D06M2101/06 ,  D06M2200/30 ,  Y10T442/2631

Abstract: The present invention is directed to methods for flameproofing cellulose fibres and articles containing these fibres. The method involves treatment of materials with cyanuric chloride.

Abstract translation: 本发明涉及用于阻燃纤维素纤维和含有这些纤维的制品的方法。 该方法涉及用氰尿酰氯处理材料。

公开(公告)号：US08022200B2

公开(公告)日：2011-09-20

申请号：US11173482

申请日：2005-07-01

Applicant: Stefan Wershofen ,  Manfred Schmidt ,  Hans-Georg Pirkl ,  Nobuhiro Kamiyama ,  Shinichi Murakami ,  Tetsuo Harada

Inventor： Stefan Wershofen ,  Manfred Schmidt ,  Hans-Georg Pirkl ,  Nobuhiro Kamiyama ,  Shinichi Murakami ,  Tetsuo Harada

IPC: C07C267/00 ,  C07D229/00

CPC classification number: C08G18/025 ,  C07C267/00 ,  C08G18/089

Abstract: The invention relates to a process for the preparation of liquid, storage-stable isocyanate mixtures which are characterized by a low color number and which contain carbodiimide (CD) and/or uretonimine (UI) groups. This invention also relates to the isocyanate mixtures obtained by this process, to their use for the preparation of blends with further isocyanates, and to the preparation of prepolymers containing isocyanate groups and of polyurethane plastics, preferably polyurethane foams from these isocyanate mixtures.

Abstract translation: 本发明涉及一种制备液体，储存稳定的异氰酸酯混合物的方法，其特征在于低色数，并含有碳二亚胺（CD）和/或脲酮亚胺（UI）基团。 本发明还涉及通过该方法获得的异氰酸酯混合物，其用于制备与另外的异氰酸酯的共混物，以及制备含有异氰酸酯基团和聚氨酯塑料的预聚物，优选来自这些异氰酸酯混合物的聚氨酯泡沫。

公开(公告)号：US07951896B2

公开(公告)日：2011-05-31

申请号：US11480596

申请日：2006-07-03

Applicant: Hartmut Nefzger ,  Manfred Schmidt ,  James-Michael Barnes ,  Ernst Felske ,  Jens Krause

Inventor： Hartmut Nefzger ,  Manfred Schmidt ,  James-Michael Barnes ,  Ernst Felske ,  Jens Krause

IPC: C08G18/00

CPC classification number: C08G18/7657 ,  C08G18/10 ,  C08G18/44 ,  C08G18/6511

Abstract: The present invention relates to NCO-terminated prepolymers that are stable to sedimentation, to a process for the production of these NCO-terminated prepolymers, and to the production of polyurethanes from these NCO-terminated polymers.

Abstract translation: 本发明涉及对沉淀稳定的NCO封端的预聚物，涉及制备这些NCO封端的预聚物的方法，以及由这些NCO封端的聚合物生产聚氨酯。

公开(公告)号：US07765948B2

公开(公告)日：2010-08-03

申请号：US12182487

申请日：2008-07-30

Applicant: Clifford L. Johns ,  Guillermo Camoriano ,  Munaf Najmuddin Chasmawala ,  Manfred Schmidt ,  Samuel Richard Gates ,  Wayne Howard Franklin

Inventor： Clifford L. Johns ,  Guillermo Camoriano ,  Munaf Najmuddin Chasmawala ,  Manfred Schmidt ,  Samuel Richard Gates ,  Wayne Howard Franklin

IPC: G09F9/00 ,  B60H1/00

CPC classification number: B01J8/06 ,  B01J8/0015 ,  B01J8/008 ,  B01J8/067 ,  B01J2208/00212 ,  B01J2208/00769 ,  B01J2208/00796 ,  B01J2219/00094 ,  Y10S40/914

Abstract: A device and method for identifying the tubes in a tubesheet, including locating a support element along a portion of the tubesheet with at least one means for identifying the location or condition of the adjacent tubes.

Abstract translation: 一种用于识别管板中的管的装置和方法，包括沿着管板的一部分定位支撑元件，具有用于识别相邻管的位置或状态的至少一个装置。

公开(公告)号：US07745659B2

公开(公告)日：2010-06-29

申请号：US11298727

申请日：2005-12-08

Applicant: Stefan Wershofen ,  Manfred Schmidt ,  Hans-Georg Pirkl ,  Torsten Hagen

Inventor： Stefan Wershofen ,  Manfred Schmidt ,  Hans-Georg Pirkl ,  Torsten Hagen

IPC: C07C267/00 ,  C07C249/00 ,  C08G18/02 ,  C08G18/08

CPC classification number: C08G18/797 ,  C08G18/025 ,  C08G18/10 ,  C08G18/4841

Abstract: The invention relates to a process for the production of liquid, storage-stable isocyanate mixtures with a low color index having carbodiimide-(CD) and/or uretone imine (UI) groups, the isocyanate mixtures that can be obtained by this process and their use for the production of blends with other isocyanates or for the production of isocyanate group-containing pre-polymers and also of polyurethane plastics, preferably polyurethane foamed plastics.

Abstract translation: 本发明涉及一种生产具有低碳指数的液体，储存稳定的异氰酸酯混合物的方法，其具有碳二亚胺 - （CD）和/或脲基亚胺（UI）基团，可通过该方法获得的异氰酸酯混合物及其 用于生产与其它异氰酸酯的共混物或用于生产含异氰酸酯基团的预聚物以及聚氨酯塑料，优选聚氨酯泡沫塑料。

公开(公告)号：US20090312545A1

公开(公告)日：2009-12-17

申请号：US12307592

申请日：2007-06-14

Applicant: Peter Werle ,  Hans-Peter Krimmer ,  Manfred Schmidt ,  Klaus Stadtmueller ,  Martin Trageser

Inventor： Peter Werle ,  Hans-Peter Krimmer ,  Manfred Schmidt ,  Klaus Stadtmueller ,  Martin Trageser

IPC: C07D251/30

CPC classification number: C07D251/34

Abstract: The invention is directed to an improved, reliably performable process for preparing triallyl isocyanurate (TAIC) by Cu2+-catalysed rearrangement of triallyl cyanurate (TAC) at least 90° C. According to the invention, TAC and, if required, also a Cu2+ catalyst and solvent are fed continuously to a start reaction mixture after onset of the initially inhibited isomerization reaction, the isomerization is performed at from 90 to 160° C. and an amount of reaction mixture equivalent to the amount of reactant is drawn off continuously and sent to the workup. Preference is given to effecting the isomerization in TAIC as the reaction medium.

Abstract translation: 本发明涉及一种改进的，可靠的可行的制备三烯丙基异氰脲酸酯（TAIC）的方法，所述三烯丙基异氰脲酸酯是通过三异丙烯酸三烯丙酯（TAC）至少90℃的Cu 2+催化重排来制备的。根据本发明，如果需要，TAC和Cu 2+催化剂 在最初抑制的异构化反应开始后，将溶剂连续加入到起始反应混合物中，异构化在90〜160℃下进行，反应混合物的量等同于反应物的量连续排出并送至 的后卫。 优选在TAIC中作为反应介质进行异构化。

公开(公告)号：US20090071751A1

公开(公告)日：2009-03-19

申请号：US11857277

申请日：2007-09-18

Applicant: FRANK SCHMIDT ,  MANFRED SCHMIDT ,  NORMAN RUST

Inventor： FRANK SCHMIDT ,  MANFRED SCHMIDT ,  NORMAN RUST

IPC: B66F11/04

CPC classification number: B66F11/04

Abstract: The invention is directed to a vertically and horizontally-controlled, retractable aerial platform mountable on the bed of a vehicle for supporting a person or materials. The aerial platform includes a base, at least one support arm, a first support member, a second support member, a platform, and actuation means. The aerial platform may further include a collapsible frame. The aerial platform is vertically and horizontally-controlled, allowing a workman to adjust both the height and extension distance of the platform so that work functions can be readily performed. The aerial platform is portable, compact and suitable for use in a variety of commercial vehicles.

Abstract translation: 本发明涉及一种可安装在车辆床上的用于支撑人或材料的垂直和水平控制的可收回的空中平台。 空中平台包括基座，至少一个支撑臂，第一支撑构件，第二支撑构件，平台和致动装置。 空中平台还可以包括可折叠框架。 空中平台是垂直和水平控制的，允许工作人员调整平台的高度和延伸距离，从而可以轻松执行工作功能。 空中平台便携，紧凑，适用于各种商业车辆。