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公开(公告)号：US20120264226A1
公开(公告)日：2012-10-18
申请号：US13253703
申请日：2011-10-05
IPC分类号： G01N21/64
CPC分类号： C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
摘要： An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
摘要翻译： 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。
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公开(公告)号：US08220487B2
公开(公告)日：2012-07-17
申请号：US11933998
申请日：2007-11-01
申请人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
发明人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
IPC分类号： F16K1/20
CPC分类号： F15C1/06 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , C12Q1/6874 , F04B43/043 , F16K1/20 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/2224 , Y10T137/87981 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译： 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。
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公开(公告)号：US20120091374A1
公开(公告)日：2012-04-19
申请号：US13278061
申请日：2011-10-20
申请人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
发明人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
IPC分类号： F16K31/12
CPC分类号： B32B3/00 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B81B3/0032 , B81B3/0051 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6874 , F04B43/043 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/2224 , Y10T137/87716 , Y10T137/87804 , Y10T137/87812 , Y10T137/87893 , Y10T156/10 , Y10T156/1002 , Y10T156/1064 , Y10T428/24479 , Y10T428/2457 , Y10T428/24612 , Y10T428/24744 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译： 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。
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公开(公告)号：US08124218B2
公开(公告)日：2012-02-28
申请号：US12556373
申请日：2009-09-09
申请人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
发明人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake
IPC分类号： B32B3/00 , F16C1/06 , F16K1/00 , F16K7/00 , F16K31/145
CPC分类号： B32B3/00 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B81B3/0032 , B81B3/0051 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6874 , F04B43/043 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/2224 , Y10T137/87716 , Y10T137/87804 , Y10T137/87812 , Y10T137/87893 , Y10T156/10 , Y10T156/1002 , Y10T156/1064 , Y10T428/24479 , Y10T428/2457 , Y10T428/24612 , Y10T428/24744 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
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5.发明申请公开(公告)号：US20110151499A1
公开(公告)日：2011-06-23
申请号：US13027768
申请日：2011-02-15
申请人： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
发明人： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
CPC分类号： G01N33/00 , B01D57/02 , B01D61/18 , B01D61/28 , B01D63/081 , B01D63/088 , B01D2313/08 , B01D2313/90 , B01F5/0646 , B01F5/0647 , B01F7/00 , B01F11/0071 , B01F13/0059 , B01F13/1013 , B01F13/1022 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502761 , B01L2200/0631 , B01L2200/10 , B01L2300/0627 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0655 , B01L2400/082 , C12M23/16 , C12M41/46 , F04B43/043 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N15/06 , G01N27/44743 , G01N27/44791 , G01N33/5005 , G01N33/5008 , G01N33/56966 , Y10T436/11 , Y10T436/117497 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
摘要翻译： 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。
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公开(公告)号：US20110151498A1
公开(公告)日：2011-06-23
申请号：US13026587
申请日：2011-02-14
申请人： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
发明人： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
CPC分类号： G01N33/00 , B01D57/02 , B01D61/18 , B01D61/28 , B01D63/081 , B01D63/088 , B01D2313/08 , B01D2313/90 , B01F5/0646 , B01F5/0647 , B01F7/00 , B01F11/0071 , B01F13/0059 , B01F13/1013 , B01F13/1022 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502761 , B01L2200/0631 , B01L2200/10 , B01L2300/0627 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0655 , B01L2400/082 , C12M23/16 , C12M41/46 , F04B43/043 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N15/06 , G01N27/44743 , G01N27/44791 , G01N33/5005 , G01N33/5008 , G01N33/56966 , Y10T436/11 , Y10T436/117497 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
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7.发明授权公开(公告)号：US07887753B2
公开(公告)日：2011-02-15
申请号：US12127720
申请日：2008-05-27
申请人： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
发明人： Stephen R. Quake , Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer
CPC分类号： G01N33/00 , B01D57/02 , B01D61/18 , B01D61/28 , B01D63/081 , B01D63/088 , B01D2313/08 , B01D2313/90 , B01F5/0646 , B01F5/0647 , B01F7/00 , B01F11/0071 , B01F13/0059 , B01F13/1013 , B01F13/1022 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502761 , B01L2200/0631 , B01L2200/10 , B01L2300/0627 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0655 , B01L2400/082 , C12M23/16 , C12M41/46 , F04B43/043 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N15/06 , G01N27/44743 , G01N27/44791 , G01N33/5005 , G01N33/5008 , G01N33/56966 , Y10T436/11 , Y10T436/117497 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
摘要翻译： 本发明提供微流体装置及其使用方法。 特别地，本发明的微流体装置可用于进行各种测定和高通量筛选。 本发明的微流体装置包括弹性体部件并且包括主流动通道; 多个分支流动通道; 多个控制信道; 和多个阀。 优选地，每个阀包括控制通道中的一个和可响应于施加到控制通道的致动力而从阀或液流通道偏转进入或从其中缩回的弹性体段。
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公开(公告)号：US20100320364A1
公开(公告)日：2010-12-23
申请号：US12862621
申请日：2010-08-24
IPC分类号： H01L27/148 , G01N21/64
CPC分类号： C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
摘要： An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
摘要翻译： 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。
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公开(公告)号：US20100263757A1
公开(公告)日：2010-10-21
申请号：US12621108
申请日：2009-11-18
申请人： David Fernandes , Hou-Pu Chou , Marc A. Unger
发明人： David Fernandes , Hou-Pu Chou , Marc A. Unger
CPC分类号： F15C3/00 , A61M2206/22 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2200/0621 , B01L2200/14 , B01L2300/123 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , F15C3/04 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0034 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0082 , F16K2099/0084 , G06F17/509 , G06F2217/16 , H01H2029/008 , Y10T137/206 , Y10T137/2202 , Y10T137/2218 , Y10T137/2224 , Y10T137/7879 , Y10T137/7891 , Y10T137/7892
摘要： A microfabricated fluidic unidirectional valve includes a microfabricated elastomer material having a flow through channel. The microfabricated fluidic unidirectional valve also includes an elastomer flap attached to the elastomer material in the flow through channel. The elastomer flap forms a seal in the flow through channel to prevent fluid from flowing in a first direction through the flow through channel and to allow fluid flow in a second direction through the flow through channel.
摘要翻译： 微制造的流体单向阀包括具有流通通道的微加工弹性体材料。 微制造的流体单向阀还包括在流动通道中连接到弹性体材料的弹性体翼片。 弹性翼片在流通通道中形成密封，以防止流体沿第一方向流过通过通道并允许流体沿第二方向流过通过通道的流体。
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公开(公告)号：US20080257437A1
公开(公告)日：2008-10-23
申请号：US12144603
申请日：2008-06-23
申请人： David N. Fernandes , Hou-Pu Chou , Marc A. Unger
发明人： David N. Fernandes , Hou-Pu Chou , Marc A. Unger
IPC分类号： F15C3/00
CPC分类号： F15C3/00 , A61M2206/22 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2200/0621 , B01L2200/14 , B01L2300/123 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , F15C3/04 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0034 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0082 , F16K2099/0084 , G06F17/509 , G06F2217/16 , H01H2029/008 , Y10T137/206 , Y10T137/2202 , Y10T137/2218 , Y10T137/2224 , Y10T137/7879 , Y10T137/7891 , Y10T137/7892
摘要： Microfabricated fluidic devices of the present invention include switches that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include high or low pressure sources. A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.
摘要翻译： 本发明的微型流体装置包括可以打开和关闭的开关，以响应于开关的门中的压力水平来允许或阻止流体通过通道的流动。 微制式流体开关可以耦合在一起以执行逻辑功能和布尔代数，例如反相器，与门，NAND，门，NOR门和或门。 逻辑门可以耦合在一起以形成锁存信号的触发器。 本发明还包括相对于第一腔室增加第二腔室中的压力的微制造流体压力倍增器。 本发明的微型流体装置还包括高压或低压源。 本发明的压力源包括通过单向阀联接到储存器的泵。 本发明的微型流体装置还可以包括执行诸如开关调节器的模拟功能的装置。
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