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    Pancreatic islet separation method, and protective solution for protecting pancreatic islet tissue
    3.
    发明授权
    Pancreatic islet separation method, and protective solution for protecting pancreatic islet tissue 有权
    胰岛分离法，保护胰岛组织保护液

    公开(公告)号：US08759099B2

    公开(公告)日：2014-06-24

    申请号：US13808390

    申请日：2011-07-13

    申请人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myoui ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

    发明人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myoui ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0676 ,  C12N2501/734

    摘要： The disclosed islet isolation method comprises: an injection step of injecting a preservation solution into the pancreatic duct of an excised pancreas; a preservation step of immersing the pancreas into an immersion fluid for preservation; a digestion step of breaking down the pancreas to provide pancreatic tissue; and a purification step of immersing the pancreatic tissue in a purification solution to provide islets. The digestion step consists of: an enzyme injection step of injecting an enzyme solution containing a digestion enzyme into the pancreas; a digestion initiation step of activating the digestion enzyme; a digestion termination step of inactivating the digestion enzyme; and a collection step of collecting the broken-down pancreatic tissue. The islet isolation method is characterized in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the time point of starting the digestion initiation step. By using the above method and a protective solution which can be used in the method, islets having a size and shape suitable for transplantation can be obtained in high yields.

    摘要翻译： 公开的胰岛分离方法包括：将保存液注入切除胰腺胰管的注射步骤; 将胰脏浸入用于保存的浸液中的保存步骤; 消化胰腺胰腺组织消化步骤; 以及将胰组织浸入净化溶液中以提供胰岛的纯化步骤。 消化步骤包括：将含有消化酶的酶溶液注入胰腺的酶注射步骤; 活化消化酶的消化引发步骤; 消化终止步骤，使消化酶失活; 以及收集分解的胰组织的收集步骤。 胰岛分离方法的特征在于，通过在消化引发步骤之前向系统中加入嗜中性粒细胞弹性蛋白酶抑制剂，在开始消化起始步骤的时间点，存在嗜中性粒细胞弹性蛋白酶抑制剂在胰腺内。 通过使用上述方法和可用于该方法的保护溶液，可以高产率获得具有适合于移植的尺寸和形状的胰岛。

    ISLETS OF LANGERHANS TRANSPLANT USING ISLETS OF LANGERHANS AND ADIPOSE TISSUE DERIVED STEM CELLS
    5.
    发明申请
    ISLETS OF LANGERHANS TRANSPLANT USING ISLETS OF LANGERHANS AND ADIPOSE TISSUE DERIVED STEM CELLS 有权
    使用兰格兰和ADIPOSE组织衍生的干细胞分离的兰格兰运输途径

    公开(公告)号：US20130171114A1

    公开(公告)日：2013-07-04

    申请号：US13814489

    申请日：2011-08-05

    申请人： Masahiro Tanemura ,  Yoshiaki Omura ,  Hiroaki Nagano ,  Masaki Mori ,  Yuichiro Doki ,  Toshinori Ito

    发明人： Masahiro Tanemura ,  Yoshiaki Omura ,  Hiroaki Nagano ,  Masaki Mori ,  Yuichiro Doki ,  Toshinori Ito

    IPC分类号： A61K35/39

    CPC分类号： A61K35/39 ,  A61K35/28 ,  C12N5/0676 ,  C12N2502/1382 ,  A61K2300/00

    摘要： An object of the present invention is to provide a pancreatic islet transplantation technique that enables increase of graft survival rate of pancreatic islets after pancreatic islet transplantation, to maintain survival of pancreatic islets, and to reduce the number of transplanted pancreatic islets required for normalizing blood glucose level. When performing pancreatic islet transplantation, by transplanting pancreatic islets and adipose tissue-derived stem cells together, it is possible to significantly improve graft survival rate of transplanted pancreatic islets, and reduce by half the number of transplanted pancreatic islets required for normalizing blood glucose level.

    摘要翻译： 本发明的目的是提供胰岛移植技术，其能够增加胰岛移植后胰岛的移植物存活率，保持胰岛的存活，并减少使血糖正常化所需的移植胰岛数量 水平。 当进行胰岛移植时，通过将胰岛和脂肪组织衍生的干细胞移植到一起，可以显着提高移植胰岛的移植物存活率，并减少标准化血糖水平所需的移植胰岛数量的一半。

    PANCREATIC ISLET SEPARATION METHOD, AND PROTECTIVE SOLUTION FOR PROTECTING PANCREATIC ISLET TISSUE
    6.
    发明申请
    PANCREATIC ISLET SEPARATION METHOD, AND PROTECTIVE SOLUTION FOR PROTECTING PANCREATIC ISLET TISSUE 有权
    胰岛分离方法和保护胰腺组织的保护性解决方案

    公开(公告)号：US20130177986A1

    公开(公告)日：2013-07-11

    申请号：US13808390

    申请日：2011-07-13

    申请人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myouri ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

    发明人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myouri ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0676 ,  C12N2501/734

    摘要： The disclosed islet isolation method comprises: an injection step of injecting a preservation solution into the pancreatic duct of an excised pancreas; a preservation step of immersing the pancreas into an immersion fluid for preservation; a digestion step of breaking down the pancreas to provide pancreatic tissue; and a purification step of immersing the pancreatic tissue in a purification solution to provide islets. The digestion step consists of: an enzyme injection step of injecting an enzyme solution containing a digestion enzyme into the pancreas; a digestion initiation step of activating the digestion enzyme; a digestion termination step of inactivating the digestion enzyme; and a collection step of collecting the broken-down pancreatic tissue. The islet isolation method is characterized in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the time point of starting the digestion initiation step. By using the above method and a protective solution which can be used in the method, islets having a size and shape suitable for transplantation can be obtained in high yields.

    摘要翻译： 公开的胰岛分离方法包括：将保存液注入切除胰腺胰管的注射步骤; 将胰脏浸入用于保存的浸液中的保存步骤; 消化胰腺胰腺组织消化步骤; 以及将胰组织浸入净化溶液中以提供胰岛的纯化步骤。 消化步骤包括：将含有消化酶的酶溶液注入胰腺的酶注射步骤; 活化消化酶的消化引发步骤; 消化终止步骤，使消化酶失活; 以及收集分解的胰组织的收集步骤。 胰岛分离方法的特征在于，通过在消化引发步骤之前向系统中加入嗜中性粒细胞弹性蛋白酶抑制剂，在开始消化起始步骤的时间点，存在嗜中性粒细胞弹性蛋白酶抑制剂在胰腺内。 通过使用上述方法和可用于该方法的保护溶液，可以高产率获得具有适合于移植的尺寸和形状的胰岛。