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12 条记录 (耗时 0.013 秒)

    ENRICHMENT METHOD OF VIRUS
    4.
    发明申请
    ENRICHMENT METHOD OF VIRUS 审中-公开
    病毒的丰富方法

    公开(公告)号：US20110053250A1

    公开(公告)日：2011-03-03

    申请号：US12935492

    申请日：2009-03-31

    申请人： Yoshimitsu Takakura ,  Masako Ichikawa ,  Kazuhiro Kondo ,  Akihiro Shimizu

    发明人： Yoshimitsu Takakura ,  Masako Ichikawa ,  Kazuhiro Kondo ,  Akihiro Shimizu

    IPC分类号： C12N7/02

    CPC分类号： C12N15/1003 ,  C12N7/00 ,  C12N2710/16051 ,  G01N2333/36

    摘要： The present invention provides a novel method that can increase readily a virus or viral vector concentration in a solution having a low concentration and a kit for performing the method. Conventional methods require complicated operations, expensive equipment, or highly trained experts for efficiently concentrating viruses from low-concentration virus solutions. The method of the present invention can concentrate viral vectors readily while maintaining infection abilities of the viral vectors, and thus it can be used as a safe and simple technique for concentrating a vector useful in the field of a genetic therapy or a vaccine therapy using a viral vector.

    摘要翻译： 本发明提供一种可以容易地增加具有低浓度的溶液中的病毒或病毒载体浓度的新方法和用于进行该方法的试剂盒。 常规方法需要复杂的操作，昂贵的设备或受过高度训练的专家，以有效地集中低浓度病毒解决方案的病毒。 本发明的方法可以容易地集中病毒载体，同时保持病毒载体的感染能力，因此它可以用作一种安全和简单的技术，用于浓缩在遗传治疗或疫苗治疗领域中有用的载体，其使用 病毒载体。

    QUANTITATION METHOD OF VIRUS
    5.
    发明申请
    QUANTITATION METHOD OF VIRUS 审中-公开
    病毒的量化方法

    公开(公告)号：US20110053145A1

    公开(公告)日：2011-03-03

    申请号：US12935429

    申请日：2009-03-31

    申请人： Yoshimitsu Takakura ,  Masako Ichikawa ,  Kazuhiro Kondo ,  Akihiro Shimizu

    发明人： Yoshimitsu Takakura ,  Masako Ichikawa ,  Kazuhiro Kondo ,  Akihiro Shimizu

    IPC分类号： C12Q1/70

    CPC分类号： G01N33/56983 ,  G01N2333/03 ,  G01N2333/36

    摘要： The present invention relates to a method of quantitatively determining the number of human herpesvirus (HHV) collected from a body fluid and a kit for performing the method. Conventionally, a trained technician has been required to accurately quantitatively determine a number of HHV collected from a body fluid. The method of the present invention is a novel method of quantitative determination that enables measurement of a number of HHV in a body fluid to be simply, accurately, and efficiently determined. The method of the present invention can enable continuous evaluation of the number of HHV in body fluids and, therefore, can be applied to quantitative evaluation of the accumulation of fatigue.

    摘要翻译： 本发明涉及一种定量确定从体液收集的人疱疹病毒（HHV）数量和执行该方法的试剂盒的方法。 通常，训练有素的技术人员需要准确地定量地确定从体液收集的HHV的数量。 本发明的方法是能够简单，精确，高效地测定体液中的HHV数量的定量测定方法。 本发明的方法能够连续评价体液中的HHV数，因此可以应用于疲劳累积的定量评价。

    Device for deciding pole-zero parameters approximating spectrum of an input signal
    6.
    发明授权
    Device for deciding pole-zero parameters approximating spectrum of an input signal 失效
    用于确定接近输入信号频谱的极点零参数的装置

    公开(公告)号：US4899386A

    公开(公告)日：1990-02-06

    申请号：US167275

    申请日：1988-03-11

    申请人： Masako Ichikawa ,  Yukio Mitome

    发明人： Masako Ichikawa ,  Yukio Mitome

    IPC分类号： G10L19/02

    CPC分类号： G10L19/02

    摘要： For use in deciding optimum pole and zero parameters for an input signal with reference to an input cepstrum of the signal, candidate pole and zero parameters are stored in advance in a table. Supplied with an impulse and controlled by a candidate set pair of a pole parameter set and a zero parameter set selected from the candidate pole and zero parameters of the table, first and second filters produce first and second outputs defined by terms of up to a certain order. Responsive to the first and second outputs and to factors of multiplication which are given by inverse numbers of time intervals related to the respective terms, an analysis filter produces a converted signal which is equivalent to a model output cepstrum of a model output signal produced by a pole-zero model defined by the candidate set pair. A cepstrum subtracter calculates a cepstrum difference between the input cepstrum and the converted signal. In connection with a few candidate set pairs, cepstrum differences and then squares of the respective cepstrum differences are calculated to decide the optimum pole and zero parameters by a focussing technique. Alternatively, a square of the cepstrum difference is used together with partial derivatives of the square to decide the optimum pole and zero parameters.

    摘要翻译： 为了参考信号的输入倒谱来确定输入信号的最佳极点和零参数，候选极点和零参数被预先存储在表中。 由脉冲提供并由候选组对极参数组和从表的候选极和零参数中选择的零参数集进行控制，第一和第二滤波器产生由多达一定值的术语定义的第一和第二输出 订购。 分析滤波器响应于第一和第二输出以及与各个项相关的时间间隔的倒数给出的乘法因子，分析滤波器产生等效于模型输出信号的模型输出倒频谱的转换信号，该模型输出信号由 由候选集合对定义的极零模型。 倒谱减法器计算输入倒谱和转换信号之间的倒谱差。 结合几个候选集合对，计算倒谱差异，然后计算各倒谱差的平方，以通过聚焦技术来确定最佳极点和零参数。 或者，将倒谱差的平方与平方的偏导数一起使用以决定最佳极点和零参数。

    MODIFIED BIOTIN-BINDING PROTEIN
    7.
    发明申请
    MODIFIED BIOTIN-BINDING PROTEIN 有权
    改良生物蛋白结合蛋白

    公开(公告)号：US20110263824A1

    公开(公告)日：2011-10-27

    申请号：US13058130

    申请日：2009-08-13

    申请人： Yoshimitsu Takakura ,  Masako Ichikawa

    发明人： Yoshimitsu Takakura ,  Masako Ichikawa

    IPC分类号： C07K14/37 ,  C07H21/04 ,  C12N15/70

    CPC分类号： C07K14/375 ,  G01N33/531 ,  G01N2333/36

    摘要： The present invention relates to a modified biotin-binding protein. The modified biotin-binding protein of the present invention includes an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one to several amino acid mutations in the sequence represented by SEQ ID NO: 2, or an amino acid sequence having 80% or more identity to the sequence represented by SEQ ID NO: 2, and having a biotin-binding activity, wherein at least one residue selected from the group consisting of: 1) an arginine residue at position 104 of SEQ ID NO: 2; 2) a lysine residue at position 141 of SEQ ID NO: 2; 3) a lysine residue at position 26 of SEQ ID NO: 2; and 4) a lysine residue at position 73 of SEQ ID NO: 2 is replaced with an acidic amino acid residue or a neutral amino acid residue.

    摘要翻译： 本发明涉及修饰的生物素结合蛋白。 本发明的修饰的生物素结合蛋白包括SEQ ID NO：2所示的氨基酸序列，在SEQ ID NO：2所示的序列中具有1〜数个氨基酸突变的氨基酸序列，或氨基酸序列 与SEQ ID NO：2表示的序列具有80％以上的同一性，并且具有生物素结合活性，其中至少一个选自以下的残基：1）SEQ ID NO：2的104位的精氨酸残基， 2; 2）SEQ ID NO：2的141位的赖氨酸残基; 3）SEQ ID NO：2的26位的赖氨酸残基; 和4）SEQ ID NO：2的第73位的赖氨酸残基被酸性氨基酸残基或中性氨基酸残基取代。

    USE OF HEAT-RESISTANT BIOTIN-BINDING PROTEIN, AND SOLID SUPPORT HAVING THE PROTEIN ATTACHED THERETO
    8.
    发明申请
    USE OF HEAT-RESISTANT BIOTIN-BINDING PROTEIN, AND SOLID SUPPORT HAVING THE PROTEIN ATTACHED THERETO 审中-公开
    耐热生物素结合蛋白的使用，以及附着于蛋白质的固体支持

    公开(公告)号：US20100330701A1

    公开(公告)日：2010-12-30

    申请号：US12521459

    申请日：2007-12-28

    申请人： Yoshimitsu Takakura ,  Satoru Usami ,  Masako Ichikawa

    发明人： Yoshimitsu Takakura ,  Satoru Usami ,  Masako Ichikawa

    IPC分类号： G01N33/543 ,  C07K14/37 ,  C07K14/76 ,  C07H21/00

    CPC分类号： C07K14/001 ,  C07K14/375 ,  C07K16/14 ,  G01N33/543 ,  G01N33/68 ,  G01N2333/36

    摘要： The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.

    摘要翻译： 本发明涉及具有附着于其上的耐热生物素结合蛋白的固体支持物。 本发明还涉及具有附着于其上的耐热生物素结合蛋白的本发明的固体支持物的用途。 本发明还涉及通过耐热生物素结合蛋白的生物素连接物质的纯化，浓缩，检测和/或捕获等技术领域。 用于本发明的固体支持物中的这种生物素结合蛋白是耐热的，因此可用于涉及暴露于70℃或更高温度的测定系统中。