-
1.发明申请Methods for enhancing survival and/or proliferation of neural stem cells and neurite extension enhancers therefor pharmaceutical compostions containing neural stem cells assay methods and screening methods 有权用于增强神经干细胞和神经突延伸增强剂的存活和/或增殖的方法，用于含有神经干细胞的药物组合物测定方法和筛选方法公开(公告)号：US20070098701A1
公开(公告)日：2007-05-03
申请号：US10571277
申请日：2004-09-08
CPC分类号： C12N5/0623 , A61K35/30 , C12N2501/59
摘要： Problems to be Solved Methods for enhancing survival and/or proliferation of neural stem cells and pharmaceutical compositions containing neural stem cells prepared by such methods, together with methods for assaying factors enhancing survival and/or proliferation of neural stem cells and methods for screening for such factors. Means for Solving the Problem Either Galectin-1 is overexpressed in neural stem cells or neural stem cells are cultured in a liquid medium containing Galectin-1. Pharmaceutical compositions containing Galectin-1-overexpressing neural stem cells and pharmaceutical composition containing Galectin-1, prepared by the aforementioned methods, improve higher cerebral functions damaged by cerebral ischemia. Further, by seeding neural stem cells at clonal concentrations and determining whether the seeded neural stem cells are capable of proliferating in an assay medium to be assayed, whether the factor enhances survival and/or proliferation of neural stem cells is assayed and a factor enhancing survival and/or proliferation of neural stem cells are identified using this assay method.
摘要翻译： 要解决的问题增强神经干细胞的存活和/或增殖的方法和通过这些方法制备的含有神经干细胞的药物组合物以及用于测定增强神经干细胞的存活和/或增殖的因子的方法以及用于筛选这些方法的方法 因素 解决问题的方法将Galectin-1在神经干细胞或神经干细胞中过表达，在含有Galectin-1的液体培养基中培养。 含有Galectin-1-过表达神经干细胞的药物组合物和含有通过上述方法制备的Galectin-1的药物组合物改善了由脑缺血损伤的较高脑功能。 此外，通过以克隆浓度接种神经干细胞并确定接种的神经干细胞是否能够在要测定的测定培养基中增殖，测定该因子是否增强神经干细胞的存活和/或增殖，并且提高生存的因子 和/或使用该测定方法鉴定神经干细胞的增殖。
-
公开(公告)号：US20100120146A1
公开(公告)日：2010-05-13
申请号：US12295814
申请日：2007-04-04
IPC分类号： C12N5/02
CPC分类号： C12N5/0623
摘要： The present invention is directed to providing the activator of the integrin signaling in neural stem cells or neural progenitors and the method for using the same. The activator of the integrin signaling comprises galectin-1 and can be used as a regulator that regulates maintenance, survival, or differentiation of a neural stem cells or a neural progenitor.
摘要翻译： 本发明涉及在神经干细胞或神经祖细胞中提供整联蛋白信号传导的激活剂及其使用方法。 整联蛋白信号传导的激活剂包含半乳凝素-1，可用作调节神经干细胞或神经祖细胞的维持，存活或分化的调节剂。
-
公开(公告)号：US07662385B2
公开(公告)日：2010-02-16
申请号：US11704185
申请日：2007-02-09
IPC分类号： C12N5/00 , C12N5/06 , A61K39/00 , A61K39/395
CPC分类号： C07K16/18 , A61K2039/505 , C07K16/2842 , C07K2317/73
摘要： The object of the present invention is to provide methods for inhibiting proliferation of neural stem cells, an agent for inhibiting proliferation of neural stem cells, and methods for using the same. According to the method of the present invention, a galectin-1 inhibitor such as anti-galectin-1 antibody and/or an integrin β1 inhibitor such as anti-integrin β1 antibody is administered to a human or a vertebrate other than human for inhibiting proliferation of neural stem cells. This method can be used for treatment of nerve injury and nerve tumors.
摘要翻译： 本发明的目的是提供抑制神经干细胞增殖的方法，抑制神经干细胞增殖的药剂及其使用方法。 根据本发明的方法，向除人以外的人或脊椎动物施用抗半凝乳素-1抗体和/或整联蛋白β1抑制剂如抗整联蛋白β1抗体的半乳凝素-1抑制剂以抑制增殖 的神经干细胞。 该方法可用于治疗神经损伤和神经瘤。
-
公开(公告)号：US20070248592A1
公开(公告)日：2007-10-25
申请号：US11704185
申请日：2007-02-09
IPC分类号： A61K39/395 , A61K39/00 , C12N5/06
CPC分类号： C07K16/18 , A61K2039/505 , C07K16/2842 , C07K2317/73
摘要： The object of the present invention is to provide methods for inhibiting proliferation of neural stem cells, an agent for inhibiting proliferation of neural stem cells, and methods for using the same. According to the method of the present invention, a galectin-1 inhibitor such as anti-galectin-1 antibody and/or an integrin β1 inhibitor such as anti-integrin β1 antibody is administered to a human or a vertebrate other than human for inhibiting proliferation of neural stem cells. This method can be used for treatment of nerve injury and nerve tumors.
摘要翻译： 本发明的目的是提供抑制神经干细胞增殖的方法，抑制神经干细胞增殖的药剂及其使用方法。 根据本发明的方法，向除人以外的人或脊椎动物施用抗半凝乳素-1抗体和/或整联蛋白β1抑制剂如抗整联蛋白β1抗体的半乳凝素-1抑制剂以抑制增殖 的神经干细胞。 该方法可用于治疗神经损伤和神经瘤。
-
5.发明授权Methods for enhancing survival and/or proliferation of neural stem cells and neurite extension enhancers therefor pharmaceutical compositions containing neural stem cells assay methods and screening methods 有权用于增强神经干细胞和神经突延伸的生存和/或增殖的方法，其增强剂，含有神经干细胞的药物组合物，测定方法和筛选方法公开(公告)号：US07785596B2
公开(公告)日：2010-08-31
申请号：US10571277
申请日：2004-09-08
申请人： Hideyuki Okano , James Hirotaka Okano , Masanori Sakaguchi , Hidehiro Mizusawa , Satoru Ishibashi
发明人： Hideyuki Okano , James Hirotaka Okano , Masanori Sakaguchi , Hidehiro Mizusawa , Satoru Ishibashi
CPC分类号： C12N5/0623 , A61K35/30 , C12N2501/59
摘要： Methods for enhancing survival and/or proliferation of neural stem cells and pharmaceutical compositions containing neural stem cells prepared by such methods, together with methods for assaying factors enhancing survival and/or proliferation of neural stem cells and methods for screening for such factors.Either Galectin-1 is overexpressed in neural stem cells or neural stem cells are cultured in a liquid medium containing Galectin-1. Pharmaceutical compositions containing Galectin-1-overexpressing neural stem cells and pharmaceutical composition containing Galectin-1, prepared by the aforementioned methods, improve higher cerebral functions damaged by cerebral ischemia. Further, by seeding neural stem cells at clonal concentrations and determining whether the seeded neural stem cells are capable of proliferating in an assay medium to be assayed, whether the factor enhances survival and/or proliferation of neural stem cells is assayed and a factor enhancing survival and/or proliferation of neural stem cells are identified using this assay method.
摘要翻译： 用于增强神经干细胞的存活和/或增殖的方法以及通过这些方法制备的含有神经干细胞的药物组合物，以及用于测定增强神经干细胞的存活和/或增殖的因子的方法以及用于筛选这些因子的方法。 在神经干细胞中Galectin-1过表达或神经干细胞在含有Galectin-1的液体培养基中培养。 含有Galectin-1-过表达神经干细胞的药物组合物和含有通过上述方法制备的Galectin-1的药物组合物改善了由脑缺血损伤的较高脑功能。 此外，通过以克隆浓度接种神经干细胞并确定接种的神经干细胞是否能够在要测定的测定培养基中增殖，测定该因子是否增强神经干细胞的存活和/或增殖，并且提高生存的因子 和/或使用该测定方法鉴定神经干细胞的增殖。
-
公开(公告)号：US20050226852A1
公开(公告)日：2005-10-13
申请号：US10509529
申请日：2003-03-27
申请人： Masahiro Toda , Hideyuki Okano , Yutaka Kawakami , Yoshiaki Toyama , Yuji Mikami , Masanori Sakaguchi
发明人： Masahiro Toda , Hideyuki Okano , Yutaka Kawakami , Yoshiaki Toyama , Yuji Mikami , Masanori Sakaguchi
IPC分类号： A61P25/00 , C12N5/02 , C12N5/0797 , A61K45/00 , C12N5/08
CPC分类号： C12N5/0623 , C12N2501/11 , C12N2501/115 , C12N2501/22 , C12N2506/11
摘要： It is intended to provide a method of efficiently inducing the growth of nerve stem cells, which are most important in transplantation therapy for nerve damage and neurological dysfunction, either in vitro or in vivo, a method of using the nerve stem cells obtained by the above growth induction method, etc. A mammalian nerve tissue containing nerve stem cells is separated and the nerve stem cells are selectively cultured in a medium containing growth factors such as EGF and FGF. Next, the nerve stem cells are co-cultured with dendritic cell such as an immature dendritic cell subset having a CD11c surface marker on the cell surface, spleen cells or blood cell-type cells such as CD8-positive T cells. Alternatively, the nerve stem cells after the culture are further cultured in the presence of GM-CSF or the nerve stem cells after the culture are further cultured in a culture supernatant of dendritic cells or a culture supernatant of blood cell-type cells.
摘要翻译： 旨在提供一种有效诱导神经干细胞生长的方法，其在体外或体内用于神经损伤和神经功能障碍的移植治疗中是最重要的，使用由上述获得的神经干细胞的方法 生长诱导方法等。分离含有神经干细胞的哺乳动物神经组织，并在含有生长因子如EGF和FGF的培养基中选择性培养神经干细胞。 接下来，将神经干细胞与树突状细胞共同培养，例如在细胞表面上具有CD11c表面标志物的未成熟树突状细胞亚群，脾细胞或血液细胞型细胞如CD8阳性T细胞。 或者，培养后的神经干细胞在GM-CSF存在下进一步培养，或者培养后的神经干细胞进一步在树突状细胞的培养上清液或血细胞型细胞的培养上清液中培养。
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.01 秒)