公开(公告)号：US20060024765A1

公开(公告)日：2006-02-02

申请号：US10535915

申请日：2003-11-21

申请人： Toshinobu Horii ,  Akira Kondo ,  Takashi Kanno ,  Masato Maekawa

发明人： Toshinobu Horii ,  Akira Kondo ,  Takashi Kanno ,  Masato Maekawa

IPC分类号： G01N33/554 ,  G01N33/569

CPC分类号： G01N33/56938

摘要： This invention relates to a method for testing S. aureus in a specimen wherein DNase produced by the S. aureus is directly detected or quantitatively determined by an immunoassay without cultivating the Staphylococcus aureus, and a test kit for testing S. aureus in a specimen by such test method. Use the present invention enables detection of S. aureus in the sample in a short time and at a high sensitivity without cultivating the S. aureus.

摘要翻译： 本发明涉及一种用于测试样品中金黄色葡萄球菌的方法，其中通过免疫测定直接检测或定量测定由金黄色葡萄球菌产生的DNA酶，而不培养金黄色葡萄球菌，以及用于通过以下方法测试样品中金黄色葡萄球菌的测试试剂盒 这种测试方法。 使用本发明能够在短时间内以高灵敏度检测样品中的金黄色葡萄球菌，而不需要培养金黄色葡萄球菌。

公开(公告)号：US20130172200A1

公开(公告)日：2013-07-04

申请号：US13686128

申请日：2012-11-27

申请人： Masato MAEKAWA ,  Takeshi Uramoto

发明人： Masato MAEKAWA ,  Takeshi Uramoto

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/158 ,  G01N33/574 ,  C12Q2545/114 ,  C12Q2535/131

摘要： A reagent for detecting gene product, the reagent comprising a probe or chemical modulation that specifically binds to an alternative splicing junction of a gene product of human PTCH1 gene, the expression of the gene product from gene products of the human PTCH1 gene being varied due to the unusual alternative splicing, for use for measuring the abundance of the gene product contained in a human sample.

摘要翻译： 用于检测基因产物的试剂，所述试剂包含特异性结合人PTCH1基因的基因产物的选择性剪接接头的探针或化学调节，所述基因产物从人PTCH1基因的基因产物的表达由于 这种不寻常的选择性剪接用于测量人样品中所含的基因产物的丰度。

公开(公告)号：US20090215035A1

公开(公告)日：2009-08-27

申请号：US11722007

申请日：2005-12-15

申请人： Masato Maekawa ,  Takeshi Uramoto

发明人： Masato Maekawa ,  Takeshi Uramoto

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/158 ,  G01N33/574 ,  C12Q2545/114 ,  C12Q2535/131

摘要： It is intended to provide a method of assessing cancerous conditions, which is useful in diagnosing cancer in a therapy or a preventive therapy for cancer diseases or a maintenance therapy therefor, and a reagent for detecting a gene product to be used in the above method. The cancerous conditions of a human-origin specimen is assessed by using, as an indication, the content of a gene product, which shows an increase or a decrease in the expression thereof due to an unusual alternative splicing, from gene products obtained from human PTCH1 gene contained in the human-origin specimen. To measure the content, use is preferably made of, concerning at least one base sequence selected from the group consisting of (a) the base sequences represented by SEQ ID NOS:1 to 4 and (b) base sequences derived from any of the base sequences represented by SEQ ID NOS:1 to 4 by deletion, substitution and/or insertion of one to several bases and having an activity of being hybridized with a chain complementary to the corresponding base sequence selected from the base sequences represented by SEQ ID NOS:1 to 4 under stringent conditions, a probe sequence-specifically binding to the complementary chain of the above-described base sequence or a chemical modification thereof.

摘要翻译： 旨在提供一种评价癌症状态的方法，其可用于诊断癌症或癌症疾病的预防疗法或其维持治疗中的癌症，以及用于检测上述方法中使用的基因产物的试剂。 作为指示，通过使用从人PTCH1获得的基因产物中的基因产物的含量，其显示由于异常选择性剪接引起的表达增加或减少，从而评估人源标本的癌症状态 基因包含在人源标本中。 为了测量含量，优选使用关于至少一种碱基序列的选择，所述碱基序列选自（a）由SEQ ID NO：1至4表示的碱基序列和（b）源自任何碱基的碱基序列 由SEQ ID NO：1至4表示的序列通过缺失，取代和/或插入一至数个碱基并具有与选自SEQ ID NO：1所代表的碱基序列的相应碱基序列互补的链进行杂交的活性。 在严格条件下1至4，特异性结合上述碱基序列的互补链的探针序列或其化学修饰。

公开(公告)号：US20060051820A1

公开(公告)日：2006-03-09

申请号：US10535917

申请日：2003-11-21

申请人： Toshinobu Horii ,  Akira Kondo ,  Takashi Kanno ,  Masato Maekawa

发明人： Toshinobu Horii ,  Akira Kondo ,  Takashi Kanno ,  Masato Maekawa

IPC分类号： G01N33/554 ,  G01N33/569

CPC分类号： G01N33/56938

摘要： This invention relates to a method for testing S. aureus in a specimen by an immunoassay using an antibody against protein A wherein at least one mouse IgG1 monoclonal antibody is used in the immunoassay, and a test kit for testing S. aureus in a specimen wherein the kit at least comprises a mouse IgG1 anti-protein A monoclonal antibody and a reagent for detecting the labeled protein A. Use the present invention enables detection of S. aureus in the sample in a short time and at a high sensitivity.

摘要翻译： 本发明涉及一种通过使用针对蛋白A的抗体的免疫测定来检测样品中的金黄色葡萄球菌的方法，其中在免疫测定中使用至少一种小鼠IgG1单克隆抗体，以及用于在样品中测试金黄色葡萄球菌的测试试剂盒， 试剂盒至少包含小鼠IgG1抗蛋白A单克隆抗体和用于检测标记蛋白A的试剂。