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    Method For Observing A Sample
    1.
    发明申请
    Method For Observing A Sample 有权
    观察样品的方法

    公开(公告)号：US20130344532A1

    公开(公告)日：2013-12-26

    申请号：US14000813

    申请日：2012-02-16

    Applicant: Mathieu Dupoy ,  Pierre Joly ,  Frédéric Mallard ,  Pierre Marcoux ,  Raphaël Mathey ,  Armelle Novelli-Rousseau

    Inventor： Mathieu Dupoy ,  Pierre Joly ,  Frédéric Mallard ,  Pierre Marcoux ,  Raphaël Mathey ,  Armelle Novelli-Rousseau

    IPC: C12Q1/04

    CPC classification number: C12Q1/04 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/035

    Abstract: A method for observing a sample is provided. The method includes the steps of: a) providing a volume (G, V) of a substantially transparent medium having a first refractive index (nint) and having a first surface (S1) that is convex, rounded, or faceted, and a second substantially planar surface (S2), the first surface separating the volume from a surrounding medium (MA) having a second refractive index (next) that is lower than the first index, the sample to be observed being contained inside the volume or being deposited on the first surface; b) observing the sample through the second substantially planar surface; and c) determining the presence of a substance or an object in the sample by detection during step b), a ring of light that correlates with the volume.

    Abstract translation: 提供了一种用于观察样品的方法。 该方法包括以下步骤：a）提供具有第一折射率（nint）并且具有凸起，圆形或小面的第一表面（S1）的基本上透明的介质的体积（G，V），以及第二 基本平坦的表面（S2），第一表面将体积与具有低于第一指数的第二折射率（next）的周围介质（MA）分开，待观察的样品被容纳在体积内或沉积在 第一面; b）通过第二基本平坦的表面观察样品; 以及c）通过在步骤b）期间通过检测与体积相关的光环来确定样品中物质或物体的存在。

    Method for Classifying the Presence or Absence of a Microorganism in a Biological Medium
    2.
    发明申请
    Method for Classifying the Presence or Absence of a Microorganism in a Biological Medium 有权
    生物培养基中存在或缺乏微生物的方法

    公开(公告)号：US20140162301A1

    公开(公告)日：2014-06-12

    申请号：US14127939

    申请日：2012-06-27

    Applicant: Pierre Marcoux ,  Mathieu Dupoy ,  Laure-Hélène Guillemot ,  Thu-Hoa Tran-Thi

    Inventor： Pierre Marcoux ,  Mathieu Dupoy ,  Laure-Hélène Guillemot ,  Thu-Hoa Tran-Thi

    IPC: C12Q1/18 ,  C12Q1/04

    CPC classification number: C12Q1/18 ,  C12Q1/04 ,  C12Q2334/10 ,  C12Q2334/30

    Abstract: The present invention relates to a method for determining the presence or absence of a microorganism, said method including the steps of: 1) providing an enclosure containing a liquid or semi-solid phase consisting of a biological medium capable of containing a living form of said microorganism, nutritional elements, and an enzymatic substrate which is specific to said microorganism and which can be metabolised into at least one VOC metabolite, and a gaseous phase adjacent to said liquid or semi-solid phase; 2) exposing at least said liquid or semi-solid phase to conditions that are propitious for said microorganism to metabolise said enzymatic substrate into a molecule of said VOC metabolite; and 3) determining, by optical transduction, the presence or absence of said VOC metabolite, characterised in that the latter interacts with a nanoporous matrix, said matrix being implemented in a form that is separate from said enzymatic substrate, and in that the detection, by optical transduction, of a change in the optical properties of said matrix indicates an interaction of said matrix with said metabolite.

    Abstract translation: 本发明涉及一种用于确定微生物存在或不存在的方法，所述方法包括以下步骤：1）提供包含液体或半固相的外壳，所述液体或半固相由能够容纳所述 微生物，营养元素和对所述微生物特异性并且可以代谢成至少一种VOC代谢物的酶底物以及与所述液体或半固相相邻的气相; 2）将至少所述液体或半固体相暴露于有利于所述微生物将所述酶底物代谢成所述VOC代谢物分子的条件; 3）通过光学转导来确定所述VOC代谢物的存在或不存在，其特征在于后者与纳米多孔基质相互作用，所述基质以与所述酶底物分开的形式实施，并且所述检测， 通过光学转导，所述基质的光学性质的变化表示所述基质与所述代谢物的相互作用。

    Method for classifying the presence or absence of a microorganism in a biological medium
    3.
    发明授权
    Method for classifying the presence or absence of a microorganism in a biological medium 有权
    在生物培养基中分类微生物的存在或不存在的方法

    公开(公告)号：US09556469B2

    公开(公告)日：2017-01-31

    申请号：US14127939

    申请日：2012-06-27

    Applicant: Pierre Marcoux ,  Mathieu Dupoy ,  Laure-Hélène Guillemot ,  Thu-Hoa Tran-Thi

    Inventor： Pierre Marcoux ,  Mathieu Dupoy ,  Laure-Hélène Guillemot ,  Thu-Hoa Tran-Thi

    IPC: C12Q1/04 ,  G01N1/22 ,  G01N21/17 ,  C12Q1/18

    CPC classification number: C12Q1/18 ,  C12Q1/04 ,  C12Q2334/10 ,  C12Q2334/30

    Abstract: Porous sol-gel material essentially consisting of units of one or more first polyalkoxysilanes chosen from the following compounds: (chloromethyl)triethoxysilane; 1,3-dimethyltetramethoxydisiloxane; ethyltrimethoxysilane; triethoxy(ethyl)silane; triethoxymethylsilane; triethoxy(vinyl)silane; trimethoxymethylsilane; trimethoxy(vinyl)silane; tetraethoxysilane or tetramethoxysilane (TMOS) and of units of one or more second polyalkoxysilanes chosen from the following compounds: (N-(3-(trimethoxysilyl)propyl)ethylenediamine; 3-aminopropyltriethoxysilane (APTES) and 3-aminopropyltrimethoxysilane, in a first polyalkoxysilane/second polyalkoxysilane molar ratio of 1/0.01 to 1/1, optionally comprising a probe molecule, method of preparation and applications in the trapping of monocyclic aromatic hydrocarbons and other pollutants or in their detection.

    Abstract translation: 多孔溶胶凝胶材料主要由选自以下化合物的一种或多种第一聚烷氧基硅烷的单元组成：（氯甲基）三乙氧基硅烷; 1,3-二甲基四甲氧基二硅氧烷; 乙基三甲氧基硅烷; 三乙氧基（乙基）硅烷; 三乙氧基甲基硅烷; 三乙氧基（乙烯基）硅烷; 三甲氧基甲基硅烷; 三乙氧基（乙烯基）硅烷; 四乙氧基硅烷或四甲氧基硅烷（TMOS）以及选自以下化合物的一种或多种第二聚烷氧基硅烷的单元：（N-（3-（三甲氧基甲硅烷基）丙基）乙二胺; 3-氨基丙基三乙氧基硅烷（APTES）和3-氨基丙基三甲氧基硅烷，在第一聚烷氧基硅烷/ 第二聚烷氧基硅烷的摩尔比为1 / 0.01至1/1，任选地包含探针分子，制备方法和捕获单环芳烃和其它污染物或其检测中的应用。

    Method for observing a sample
    4.
    发明授权
    Method for observing a sample 有权
    观察样品的方法

    公开(公告)号：US09359631B2

    公开(公告)日：2016-06-07

    申请号：US14000813

    申请日：2012-02-16

    Applicant: Mathieu Dupoy ,  Pierre Joly ,  Frédéric Mallard ,  Pierre Marcoux ,  Raphaël Mathey ,  Armelle Novelli-Rousseau

    Inventor： Mathieu Dupoy ,  Pierre Joly ,  Frédéric Mallard ,  Pierre Marcoux ,  Raphaël Mathey ,  Armelle Novelli-Rousseau

    IPC: C12Q1/04 ,  G01N21/63 ,  G01N21/43 ,  G01N21/41 ,  G01N21/64 ,  G01N21/65 ,  G01N21/03

    CPC classification number: C12Q1/04 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/035

    Abstract: A method for observing a sample is provided. The method includes the steps of: a) providing a volume (G, V) of a substantially transparent medium having a first refractive index (nint) and having a first surface (S1) that is convex, rounded, or faceted, and a second substantially planar surface (S2), the first surface separating the volume from a surrounding medium (MA) having a second refractive index (nest) that is lower than the first index, the sample to be observed being contained inside the volume or being deposited on the first surface; b) observing the sample through the second substantially planar surface; and c) determining the presence of a substance or an object in the sample by detection during step b), a ring of light that correlates with the volume.

    Abstract translation: 提供了一种用于观察样品的方法。 该方法包括以下步骤：a）提供具有第一折射率（nint）并且具有凸起，圆形或小面的第一表面（S1）的基本上透明的介质的体积（G，V），以及第二 基本上平坦的表面（S2），所述第一表面将体积与具有低于第一指数的第二折射率（巢）的周围介质（MA）分离，待观察的样品包含在体积内或沉积在 第一面; b）通过第二基本平坦的表面观察样品; 以及c）通过在步骤b）期间通过检测与体积相关的光环来确定样品中物质或物体的存在。

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