公开(公告)号：US20220220471A1

公开(公告)日：2022-07-14

申请号：US17615311

申请日：2020-06-03

申请人： Di Wu ,  Tomasz Wirt ,  Mats Nilsson Bernitz

发明人： Di Wu ,  Tomasz Wirt ,  Mats Nilsson Bernitz

IPC分类号： C12N15/10 ,  C12Q1/6855 ,  C12Q1/6806

摘要： The present invention relates to the field of biotechnology and a method for creating a cDNA library. More specifically, the invention refers to a method of forming complementary DNA (cDNA) sequencing libraries from RNA in situ comprising the steps of: (a) fixating cells, immobilized on a solid surface; (b) performing an in situ reverse transcription (RT) inside cells, using RT primers comprising a PCR handle 1, and partially biotinylated dNTPs; (c) releasing single stranded cDNA from the cells using a release mix, wherein the release mix comprises an RNAse, such that the single stranded cDNA is released from intact cells; (d) collecting a supernatant comprising released cDNA into a single larger volume or in separate volumes; and (e) introducing an adapter molecule comprising a PCR handle 2 that will bind 3′ of the extended cDNA.

公开(公告)号：US09464855B2

公开(公告)日：2016-10-11

申请号：US14378443

申请日：2012-02-13

申请人： Mats Nilsson

发明人： Mats Nilsson

IPC分类号： F41A1/10 ,  F41F3/042

CPC分类号： F41A1/10 ,  F41F3/042

摘要： A countermass container for use in a recoilless weapon. The countermass container includes an envelope encloses a countermass. A recoilless weapon includes a barrel accommodating an ammunition unit, a propellant charge and a countermass container. The countermass container includes an envelope enclosing a countermass. The barrel includes a front end opening for firing the ammunition unit out of the barrel and a rear end opening for ejecting the envelope and countermass of the counter mass container. The envelope is divided into a front section and a rear section. The rear section of the envelope has a weaker construction than the front section of the envelope to more easily crack than the front section of the envelope. Splines are provided at the front section to create ducts.

摘要翻译： 用于无后坐力武器的反沙箱。 反质量容器包括一个信封，包围一个反质量。 无后坐力武器包括容纳弹药单元的桶，推进剂装料和反质量容器。 反对质量容器包括一个包围反对质量的信封。 枪管包括用于将弹药单元从枪管射出的前端开口和用于弹出柜体的容器的信封和反质量的后端开口。 信封分为前部和后部。 信封的后部部分的结构比信封的前部部分薄，比信封的前部更容易裂开。 在前部提供花键以创建导管。

公开(公告)号：US08551710B2

公开(公告)日：2013-10-08

申请号：US13542378

申请日：2012-07-05

申请人： Mats Nilsson Bernitz ,  Chatarina Larsson ,  Ida Grundberg

发明人： Mats Nilsson Bernitz ,  Chatarina Larsson ,  Ida Grundberg

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6827 ,  C12Q1/6809 ,  C12Q1/6841 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2521/107 ,  C12Q2521/327 ,  C12Q2525/107 ,  C12Q2525/125 ,  C12Q2525/161 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/143 ,  C12Q2543/10

摘要： The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.

摘要翻译： 本发明涉及细胞样品中RNA的检测。 更具体地，本发明涉及原位RNA的局部检测。 该方法依赖于用挂锁探针靶向cDNA之前RNA到互补DNA的转化。 挂锁探针的杂交依赖于衍生自靶RNA的相应核苷酸序列的cDNA的核苷酸序列。 随后环形化的挂锁探针的滚环放大产生可被检测的滚动圆形产品。 有利地，这允许原位检测RNA。

公开(公告)号：US08443341B2

公开(公告)日：2013-05-14

申请号：US11938247

申请日：2007-11-09

申请人： Erik Berg ,  Erik Hagersten ,  Mats Nilsson ,  Mikael Petterson ,  Magnus Vesterlund ,  Hakan Zeffer

发明人： Erik Berg ,  Erik Hagersten ,  Mats Nilsson ,  Mikael Petterson ,  Magnus Vesterlund ,  Hakan Zeffer

IPC分类号： G06F9/44

CPC分类号： G06F11/3616 ,  G06F12/0802

摘要： A system for, method of and computer program product captures performance-characteristic data from the execution of a program and models system performance based on that data. Performance-characterization data based on easily captured reuse distance metrics is targeted, defined as the total number of memory references between two accesses to the same piece of data. Methods for efficiently capturing this kind of metrics are described. These data can be refined into easily interpreted performance metrics, such as performance data related to caches with LRU replacement and random replacement strategies in combination with fully associative as well as limited associativity cache organizations. Methods for assessing cache utilization as well as parallel execution are covered.

摘要翻译： 系统，方法和计算机程序产品从执行程序中捕获性能特征数据，并根据该数据对系统性能进行建模。 基于容易捕获的复用距离度量的性能表征数据被定义为两次访问同一数据块之间的存储器引用的总数。 描述有效捕获这种度量的方法。 这些数据可以被简化为易于解释的性能指标，例如与具有LRU替换的缓存和随机替换策略相关联的性能数据以及完全关联以及有限关联缓存组织。 涵盖了评估缓存利用率和并行执行的方法。

公开(公告)号：US20130004953A1

公开(公告)日：2013-01-03

申请号：US13542378

申请日：2012-07-05

申请人： Mats Nilsson Bernitz ,  Chatarina Larsson ,  Ida Gundberg

发明人： Mats Nilsson Bernitz ,  Chatarina Larsson ,  Ida Gundberg

IPC分类号： C12Q1/68 ,  G01N21/64

CPC分类号： C12Q1/6827 ,  C12Q1/6809 ,  C12Q1/6841 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2521/107 ,  C12Q2521/327 ,  C12Q2525/107 ,  C12Q2525/125 ,  C12Q2525/161 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/143 ,  C12Q2543/10

摘要： The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.

摘要翻译： 本发明涉及细胞样品中RNA的检测。 更具体地，本发明涉及原位RNA的局部检测。 该方法依赖于用挂锁探针靶向cDNA之前RNA到互补DNA的转化。 挂锁探针的杂交依赖于衍生自靶RNA的相应核苷酸序列的cDNA的核苷酸序列。 随后环形化的挂锁探针的滚环放大产生可被检测的滚动圆形产品。 有利地，这允许原位检测RNA。

公开(公告)号：US20120103266A1

公开(公告)日：2012-05-03

申请号：US13381755

申请日：2010-06-24

申请人： Mats Nilsson

发明人： Mats Nilsson

IPC分类号： A01J5/003 ,  A01J5/007

CPC分类号： A01K1/126

摘要： An arrangement in a rotary milking system includes a rotating platform, an entry thereto and an exit therefrom. A selection gate arrangement at the exit of the rotating platform selectively guides animals, which have been milked in the rotary milking system and leave the rotating platform through the exit, back to the entry of the rotating platform or to a waiting area. A control device is provided for (i) determining whether an animal, which has been milked and leaves the rotating platform through the exit, has at least one udder compartment that was incompletely milked the last time the animal was milked, and (ii) controlling the selection gate arrangement to guide the animal back to the entry of the rotating platform or to the waiting area provided that the animal has at least one udder compartment that was incompletely milked the last time the animal was milked.

摘要翻译： 旋转挤奶系统中的布置包括旋转平台，入口和从其中的出口。 在旋转平台的出口处的选择门装置选择性地引导已经在旋转挤奶系统中挤奶的动物，并使旋转平台离开出口，返回到旋转平台的入口或等待区域。 提供控制装置，用于（i）确定已经挤奶并离开旋转平台通过出口的动物是否具有在上次动物挤奶时不完全挤奶的至少一个乳房隔室，以及（ii）控制 选择门装置，用于将动物引导回到旋转平台或等待区域的入口，只要动物在上次动物被挤奶时具有不完全挤奶的至少一个乳房隔间。

公开(公告)号：US20110120379A1

公开(公告)日：2011-05-26

申请号：US12929150

申请日：2011-01-04

申请人： Lars Wase ,  Mats Nilsson ,  Nils Erik Holmertz ,  Jan-Erik Andersson ,  Krister Sjöblom ,  Mattias Oscarsson

发明人： Lars Wase ,  Mats Nilsson ,  Nils Erik Holmertz ,  Jan-Erik Andersson ,  Krister Sjöblom ,  Mattias Oscarsson

IPC分类号： B25J15/06

CPC分类号： A01J5/0175

摘要： A griper device mountable on a robot arm of a milking robot provided for automatically attach teat cups to the teats of a milking animal, the gripper device comprises an electromagnet from gripping teat cups made of a magnetic material, the electromagnet including a coil feedable with a current a core of a magnetic material arranged at least partly within the coil; and a support structure for holding the electromagnet. The core comprises two end and portions in a single face of the electromagnet wherein the two end portions constitute different poles of the electromagnet and are shaped to be engagable with each of the teat cups in a close fit.

摘要翻译： 一种安装在挤奶机器人的机器人手臂上的抓手装置，用于将挤奶杯自动地连接到挤奶动物的乳头上，该夹持装置包括由磁性材料制成的夹持奶杯的电磁体，该电磁体包括可由 电流为至少部分地布置在线圈内的磁性材料的芯; 以及用于保持电磁体的支撑结构。 芯部包括电磁体的单个面中的两个端部和部分，其中两个端部构成电磁体的不同极，并且被成形为能够以紧密配合的每个奶杯接合。

公开(公告)号：US07908998B2

公开(公告)日：2011-03-22

申请号：US11630247

申请日：2005-06-16

申请人： Lars Wase ,  Mats Nilsson ,  Nils Erik Holmertz ,  Jan-Erik Andersson ,  Krister Sjöblom ,  Mattias Oscarsson

发明人： Lars Wase ,  Mats Nilsson ,  Nils Erik Holmertz ,  Jan-Erik Andersson ,  Krister Sjöblom ,  Mattias Oscarsson

IPC分类号： A01J5/017

CPC分类号： A01J5/0175

摘要： A gripper device mountable on a robot arm (10) of a milking robot (2) provided for automatically attach teat cups (5) to the teats of a milking animal, the gripper device comprises an electromagnet (6) from gripping teat cups made of a magnetic material, the electromagnet including a coil (31) feedable with a current a core (33) of a magnetic material arranged at least partly within the coil; and a support structure (7) for holding the electromagnet. The core comprises two end and portions (33a-b) in a single face of the electromagnet, wherein the two end portions constitute different poles of the electromagnet, and are shaped to be engagable with each of the teat cups in a close fit.

摘要翻译： 夹持器装置可安装在挤奶机器人（2）的机器人臂（10）上，用于将挤奶杯（5）自动地连接到挤奶动物的乳头上，夹持装置包括一个电动磁铁（6） 一种磁性材料，所述电磁体包括线圈（31），所述线圈（31）可馈送至少部分地在所述线圈内布置的磁性材料的芯体（33） 以及用于保持电磁体的支撑结构（7）。 芯部包括在电磁体的单个面中的两个端部和部分（33a-b），其中两个端部构成电磁体的不同的极，并且被成形为能够以紧密配合的每个奶嘴杯接合。

公开(公告)号：US20100087572A1

公开(公告)日：2010-04-08

申请号：US12572830

申请日：2009-10-02

申请人： Mats Nilsson ,  Truls Nilsson

发明人： Mats Nilsson ,  Truls Nilsson

IPC分类号： C08K3/32 ,  C08L3/02

CPC分类号： C09K21/12 ,  C08K5/0066 ,  C08K9/10

摘要： A method for producing an all purpose harmless fire retardant protein free composition that is adaptable to be incorporated into a range of differing material, of which said material forms an intermediate or finished product requiring fire retardant characteristics, said method including the steps of, (A) dissolving a mono-carboxylic acid, selected from a group, consisting of at least one of, or a combination therefore, of formic acid, acetic acid, stearic acid and/or benzoic acid, and/or an anhydrous di-carboxylic acid selected from a group, consisting of at least one of, or a combination therefore, of oxalic acid, malonic acid, succinic acid and/or glutaric acid, and/or an anhydrous tri-carboxylic acid, consisting of citric acid in an acetic acid solvent forming a solution with a pH in the range of 1.0 to 3.0, (B) adding tetra-sodium-pyrophosphate and water to A for an endothermic process, (C) adding calcium carbonates to the acidic solution B for the generation of an exothermic reaction, (D) once composition C becomes clumpy the composition is poured out onto a drying bed for drying, (E) the dried composition of step D is grounded, milled and/or grinded to achieve particles of less than 0.020 m, (F) encapsulate the particles of E with a surface coating material selected from a group, consisting of at least one of, or a combination therefore, of polysaccharide, tristearate and/or a wax of a polyethylene based material. (G) such that the encapsulation particulate crystals are blended during the formation of the intermediate or finished polymeric material in that the encapsulation provides means for the particulate crystals to remain inactive within and when applied to the polymeric material until said polymeric material is ignited by fire.

摘要翻译： 一种生产适用于不同材料范围的各种无害阻燃蛋白质组合物的方法，其中所述材料形成需要阻燃特性的中间体或成品，所述方法包括以下步骤：（A ）溶解选自由选自下组中的至少一种或其组合的单羧酸，即，选自甲酸，乙酸，硬脂酸和/或苯甲酸和/或无水二羧酸中的至少一种或其组合 从由乙酸溶剂中的柠檬酸组成的草酸，丙二酸，琥珀酸和/或戊二酸，和/或无水三羧酸中的至少一种或组合组成的组中， 形成pH在1.0至3.0范围内的溶液，（B）向A中加入焦磷酸四钠和水用于吸热过程，（C）向酸性溶液B中加入碳酸钙以产生放热反应 （D）一旦组合物C变得团块化，将组合物倒出到干燥床上进行干燥，（E）将步骤D的干燥组合物接地，研磨和/或研磨以获得小于0.020μm的颗粒（F ）用选自由聚乙烯基材料的多糖，三硬脂酸酯和/或蜡中的至少一种或组合组成的组的表面涂层材料包封E的颗粒。 （G），使得在形成中间体或成品聚合物材料期间将包封颗粒晶体共混，因为包封提供了使颗粒状晶体在施加到聚合物材料之前和当应用于聚合材料时保持无活性的手段，直到所述聚合物材料被火点燃 。

公开(公告)号：US20080244533A1

公开(公告)日：2008-10-02

申请号：US12055996

申请日：2008-03-26

申请人： Erik Berg ,  Erik Hagersten ,  Hakan Zeffer ,  Magnus Vesterlund ,  Mats Nilsson ,  Mikael Petterson

发明人： Erik Berg ,  Erik Hagersten ,  Hakan Zeffer ,  Magnus Vesterlund ,  Mats Nilsson ,  Mikael Petterson

IPC分类号： G06F9/44

CPC分类号： G06F11/3447 ,  G06F11/3466 ,  G06F2201/88 ,  G06F2201/885

摘要： A system for, method of and computer program product captures performance-characteristic data from the execution of a program and models system performance based on that data. Performance-characterization data based on easily captured reuse distance metrics is targeted, defined as the total number of memory references between two accesses to the same piece of data. Methods for efficiently capturing this kind of metrics are described. These data can be refined into easily interpreted performance metrics, such as performance data related to caches with LRU replacement and random replacement strategies in combination with fully associative as well as limited associativity cache organizations. Methods for assessing cache utilization as well as parallel execution are covered.

摘要翻译： 系统，方法和计算机程序产品从执行程序中捕获性能特征数据，并根据该数据对系统性能进行建模。 基于容易捕获的复用距离度量的性能表征数据被定义为两次访问同一数据块之间的存储器引用的总数。 描述有效捕获这种度量的方法。 这些数据可以被简化为易于解释的性能指标，例如与具有LRU替换的缓存和随机替换策略相关联的性能数据以及完全关联以及有限关联缓存组织。 涵盖了评估缓存利用率和并行执行的方法。