公开(公告)号：US5354690A

公开(公告)日：1994-10-11

申请号：US630563

申请日：1990-12-20

申请人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Matti Hoyhtya

发明人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Matti Hoyhtya

IPC分类号： C07K14/78 ,  C12Q1/68 ,  G01N33/53 ,  C07K15/00 ,  C12Q1/00 ,  G01N33/569

CPC分类号： C12Q1/6883 ,  C07K14/78

摘要： The present invention provides for a method for preparing antibodies that specifically detect the type IV collagen .alpha.5 chain that is defective in various basement membrane disorders such as in X chromosome-linked Alport's syndrome. The invention further provides for the use of such antibodies to detect the .alpha.5(IV) collagen chain in solutions and other human tissue specimens using immunological methods comprised of antibodies specific for said protein. Along this line, the invention relates to the use of the specific antibodies to examine the presence or absence of the .alpha.5(IV) collagen chain in the tissues, e.g. skin or kidneys, etc. of patients with renal failure, possibly due to Alport's syndrome.

摘要翻译： 本发明提供了一种制备抗体的方法，所述抗体特异性检测了各种基底膜病症如X染色体连锁的阿尔波特综合征中有缺陷的IV型胶原α5链。 本发明进一步提供了使用这种抗体以使用由所述蛋白质特异性抗体组成的免疫学方法来检测溶液和其它人体组织标本中的α5（IV）胶原链。 沿着这条线，本发明涉及特异性抗体用于检查组织中α5（IV）胶原链的存在或不存在的情况，例如， 肾衰竭患者的皮肤或肾脏等，可能是由于阿尔波特综合症。

公开(公告)号：US07749712B2

公开(公告)日：2010-07-06

申请号：US10535260

申请日：2003-11-17

申请人： Timo Pulli ,  Matti Hoyhtya ,  Kristiina Takkinen ,  Hans Soderlund

发明人： Timo Pulli ,  Matti Hoyhtya ,  Kristiina Takkinen ,  Hans Soderlund

IPC分类号： G01N33/00 ,  G01N33/53

CPC分类号： C07K16/44 ,  C07K2317/622 ,  G01N33/542 ,  G01N33/6857 ,  G01N33/946 ,  G01N33/948 ,  G01N33/9486

摘要： A non-competitive immunoassay for small analytes, wherein the analyte is reacted with two binding partners. The first binding partner binds to the analyte to form a complex between the first binding partner and the analyte, and the second binding partner binds to the complex formed by the first binding partner and the analyte. The resulting complex formed between the analyte and the binding partners is detected. The binding partners are proteins, such as antibodies including antibody fragments.

摘要翻译： 用于小分析物的非竞争性免疫测定法，其中分析物与两个结合配偶体反应。 第一结合配偶体与分析物结合以在第一结合配偶体和分析物之间形成复合物，并且第二结合配偶体与由第一结合配偶体和分析物形成的复合物结合。 检测在分析物和结合配偶体之间形成的所得复合物。 结合配偶体是蛋白质，例如包括抗体片段的抗体。

公开(公告)号：US20060246506A1

公开(公告)日：2006-11-02

申请号：US10535260

申请日：2003-11-17

申请人： Timo Pulli ,  Matti Hoyhtya ,  Kristiina Takkinen ,  Hans Soderlund

发明人： Timo Pulli ,  Matti Hoyhtya ,  Kristiina Takkinen ,  Hans Soderlund

IPC分类号： G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C40B30/06 ,  C07K16/18 ,  C12N5/06

CPC分类号： C07K16/44 ,  C07K2317/622 ,  G01N33/542 ,  G01N33/6857 ,  G01N33/946 ,  G01N33/948 ,  G01N33/9486

摘要： A non-competitive immunoassay for small analytes, wherein the analyte is reacted with two binding partners. The first binding partner binds to the analyte to form a complex between the first binding partner and the analyte, and the second binding partner binds to the complex formed by the first binding partner and the analyte. The resulting complex formed between the analyte and the binding partners is detected. The binding partners are proteins, such as antibodies including antibody fragments.

摘要翻译： 用于小分析物的非竞争性免疫测定法，其中分析物与两个结合配偶体反应。 第一结合配偶体与分析物结合以在第一结合配偶体和分析物之间形成复合物，并且第二结合配偶体与由第一结合配偶体和分析物形成的复合物结合。 检测在分析物和结合配偶体之间形成的所得复合物。 结合配偶体是蛋白质，例如包括抗体片段的抗体。

公开(公告)号：US5593900A

公开(公告)日：1997-01-14

申请号：US321084

申请日：1994-10-11

申请人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Matti Hoyhtya

发明人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Matti Hoyhtya

IPC分类号： C07K14/78 ,  C12Q1/68 ,  C12P21/00 ,  C07K16/18

CPC分类号： C12Q1/6883 ,  C07K14/78

摘要： The present invention provides for a method for preparing antibodies that specifically detect the type IV collagen .alpha.5 chain that is defective in various basement membrane disorders such as in X chromosome-linked Alport's syndrome. The invention further provides for the use of such antibodies to detect the .alpha.5(IV) collagen chain in solutions and other human tissue specimens using immunological methods comprised of antibodies specific for said protein. Along this line, the invention relates to the use of the specific antibodies to examine the presence or absence of the .alpha.5(IV) collagen chain in the tissues, e.g. skin or kidneys, etc. of patients with renal failure, possibly due to Alport's syndrome. In addition, a further object of the present invention is to provide compositions, comprising both labeled and unlabeled peptides containing sequences from the .alpha.5(IV) chain antigen (either as single peptides or conjugated to a carrier molecule or matrix) or labeled or unlabeled antibodies to said .alpha.5(IV) collagen chain or peptides containing sequence specific for the said polypeptide chain, and the use of the compositions for the diagnosis of basement membrane disorders, such as Alport's syndrome.

摘要翻译： 本发明提供了一种制备抗体的方法，所述抗体特异性检测了各种基底膜病症如X染色体连锁的阿尔波特综合征中有缺陷的IV型胶原α5链。 本发明进一步提供了使用这种抗体以使用由所述蛋白质特异性抗体组成的免疫学方法来检测溶液和其它人体组织标本中的α5（IV）胶原链。 沿着这条线，本发明涉及特异性抗体用于检查组织中α5（IV）胶原链的存在或不存在的情况，例如， 肾衰竭患者的皮肤或肾脏等，可能是由于阿尔波特综合症。 此外，本发明的另一个目的是提供包含标记的和未标记的肽的组合物，其含有来自α5（IV）链抗原的序列（作为单一肽或与载体分子或基质缀合）或标记或未标记 对所述α5（IV）胶原链或含有针对所述多肽链特异性序列的肽的抗体，以及所述组合物用于诊断基底膜疾病如阿尔波特综合征的用途。