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    Method for preparing a target protein using the sHSPs
    1.
    发明授权
    Method for preparing a target protein using the sHSPs 有权
    使用sHSP制备靶蛋白的方法

    公开(公告)号：US08067552B2

    公开(公告)日：2011-11-29

    申请号：US11915314

    申请日：2005-05-23

    申请人： Sang Yup Lee ,  Mee Jung Han

    发明人： Sang Yup Lee ,  Mee Jung Han

    IPC分类号： C07K1/14

    CPC分类号： C07K1/14

    摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.

    摘要翻译： 本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。 根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。 此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sIISP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

    Method for producing target proteins by deleting or amplifying ibpA and/or ibpB gene coding for inclusion body-associated proteins
    2.
    发明授权
    Method for producing target proteins by deleting or amplifying ibpA and/or ibpB gene coding for inclusion body-associated proteins 失效
    通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法

    公开(公告)号：US07291325B2

    公开(公告)日：2007-11-06

    申请号：US10545849

    申请日：2003-07-10

    申请人： Sang Yup Lee ,  Mee Jung Han ,  Si Jae Park

    发明人： Sang Yup Lee ,  Mee Jung Han ,  Si Jae Park

    IPC分类号： A61K48/00 ,  A01N63/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12P21/02 ,  C07K14/245 ,  C07K14/5434 ,  C07K14/57 ,  C07K14/65 ,  C12N15/70

    摘要： A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described. The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.

    摘要翻译： 通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法。 描述了使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的两种方法。 第一种方法使用ibpA和/或ibpB基因缺失的细菌增强了靶蛋白的分泌生产和活性。 第二种方法增强细胞质中靶蛋白的产生，并使用ibpA和/或ibpB基因扩增的细菌将靶蛋白从可溶形式转化为不溶性包涵体。

    Composition for protecting proteins degradation comprising small heat shock proteins (sHSPs) and method of two-dimensional gel electrophoresis using the sHSPs
    3.
    发明授权
    Composition for protecting proteins degradation comprising small heat shock proteins (sHSPs) and method of two-dimensional gel electrophoresis using the sHSPs 失效
    用于保护包含小热休克蛋白（sHSP）的蛋白质降解的组合物和使用sHSPs的二维凝胶电泳方法

    公开(公告)号：US07148334B2

    公开(公告)日：2006-12-12

    申请号：US10791059

    申请日：2004-03-02

    申请人： Sang Yup Lee ,  Mee-Jung Han ,  Si Jae Park

    发明人： Sang Yup Lee ,  Mee-Jung Han ,  Si Jae Park

    IPC分类号： C07K1/26

    CPC分类号： C07K14/195 ,  C07K14/00

    摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.

    摘要翻译： 本发明涉及含有用于预防蛋白质降解的sHSP和用于二维（2-D）凝胶电泳的组合物的组合物。 此外，本发明涉及2-D凝胶电泳的改进方法，其特征在于使用sHSP。 根据本发明，在2-D凝胶电泳中防止了蛋白质斑点的减少，从而获得了具有更多蛋白质斑点的2-D凝胶。

    Method For Preparing a Target Protein Using the Shsps
    4.
    发明申请
    Method For Preparing a Target Protein Using the Shsps 有权
    使用Shsps制备靶蛋白的方法

    公开(公告)号：US20080199907A1

    公开(公告)日：2008-08-21

    申请号：US11915314

    申请日：2005-05-23

    申请人： Sang-Yup Lee ,  Mee Jung Han

    发明人： Sang-Yup Lee ,  Mee Jung Han

    IPC分类号： C12P21/04 ,  C07K16/00

    CPC分类号： C07K1/14

    摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.

    摘要翻译： 本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。 根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。 此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sIISP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

    Process For Preparing Serine-Rich Protein Employing Cysteine Synthase (CYSK) Gene
    5.
    发明申请
    Process For Preparing Serine-Rich Protein Employing Cysteine Synthase (CYSK) Gene 审中-公开
    制备使用半胱氨酸合酶（CYSK）基因的富含丝氨酸蛋白的方法

    公开(公告)号：US20090142804A1

    公开(公告)日：2009-06-04

    申请号：US12271003

    申请日：2008-11-14

    申请人： Sang Yup LEE ,  Mee-Jung Han

    发明人： Sang Yup LEE ,  Mee-Jung Han

    IPC分类号： C12P21/04 ,  C12N1/21

    CPC分类号： C07K14/5434 ,  C07K14/5759 ,  C12N15/70 ,  C12P21/02

    摘要： The present invention relates to a process for preparing a serine-rich foreign protein comprising culturing a bacterium containing the cysteine synthase (cysK) gene and a gene encoding the serine-rich foreign protein. The present invention comprises the steps of culturing a bacterium transformed with an expression vector containing a gene encoding a serine-rich foreign protein and an expression vector containing the cysK gene, or a bacterium transformed with an expression vector containing the cysK gene and a gene encoding a serine-rich foreign protein and isolating the foreign protein therefrom. The present invention is expected to be widely used to increase the production yield of a serine-rich foreign protein.

    摘要翻译： 本发明涉及一种制备富含丝氨酸的外源蛋白的方法，包括培养含有半胱氨酸合成酶（cysK）基因的细菌和编码富含丝氨酸的外源蛋白的基因。 本发明包括以下步骤：培养用含有编码富含丝氨酸的外源蛋白的基因的表达载体转化的细菌和含有cysK基因的表达载体或用含有cysK基因的表达载体转化的细菌和编码 富含丝氨酸的外源蛋白质并从其中分离外来蛋白质。 预期本发明被广泛用于提高富含丝氨酸的外源蛋白质的产量。

    Method for increasing survival rate of cells in animal cell culture under hypoxia condition
    6.
    发明授权
    Method for increasing survival rate of cells in animal cell culture under hypoxia condition 失效
    在低氧条件下提高动物细胞培养细胞存活率的方法

    公开(公告)号：US06818625B2

    公开(公告)日：2004-11-16

    申请号：US10181221

    申请日：2002-07-12

    申请人： Jongwon Lee ,  Kyu-Won Kim ,  Mee-Jung Han ,  Moo Hwan Cho ,  Yang-Il Kim

    发明人： Jongwon Lee ,  Kyu-Won Kim ,  Mee-Jung Han ,  Moo Hwan Cho ,  Yang-Il Kim

    IPC分类号： A61K3170

    CPC分类号： A61K31/7036 ,  A61K31/00 ,  A61K31/496 ,  A61K31/5383 ,  A61K31/65

    摘要： The present invention relates to a method for increasing survival rate of cells in animal cell culture under hypoxia condition by adding antibiotics to the culture media. The method of present invention comprises a step of culturing animal cells in culture media containing antibacterial agent of quinolones, quinones, aminoglycosides or chloramphenicol at the concentration range of 0.1 to 1000 &mgr;g/ml. The invented method can be practically applied for high-density animal cell culture to produce recombinant proteins or cultured cells.

    摘要翻译： 本发明涉及通过向培养基中加入抗生素来提高动物细胞在缺氧条件下培养细胞存活率的方法。 本发明的方法包括在0.1至1000mug / ml的浓度范围内培养含有喹诺酮类，醌类，氨基糖苷类或氯霉素类抗菌剂的培养基中的动物细胞的步骤。 本发明的方法可以实际应用于高密度动物细胞培养以产生重组蛋白或培养细胞。