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    Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
    1.
    发明授权
    Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same 失效
    使用其自动分析设备和检测细胞表面蛋白质和/或细胞质受体功能的测定方法

    公开(公告)号：US06372183B1

    公开(公告)日：2002-04-16

    申请号：US08870537

    申请日：1997-06-06

    申请人： Michael Anthony Akong ,  Michael Miller Harpold ,  Gonul Velicelebi ,  Paul Brust

    发明人： Michael Anthony Akong ,  Michael Miller Harpold ,  Gonul Velicelebi ,  Paul Brust

    IPC分类号： G01N2164

    CPC分类号： G01N33/6872 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058 ,  G01N35/028 ,  G01N35/109 ,  G01N2035/042 ,  G01N2333/705 ,  G01N2500/00 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/11 ,  Y10T436/115831

    摘要： Automated measurement apparatus and methods for automated drug screening procedures are provided. The apparatus is capable of initiating and measuring rapid or transient events, such as cell receptor and/or ion channel activity. In particular, the apparatus is capable of aligning with predetermined positions, one or more samples contained in a multi-well container, initiating the reaction with reagent addition and measuring a resultant attribute for a period of time. The apparatus can substantially continuously measure and record data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event may be determined. After the reaction(s) are complete in one or more wells of the multi-well container, the apparatus can align one or more different wells to be assayed with the predetermined position and repeat the cycle until a predetermined number of wells are assayed. Automated drug screening methods employing the apparatus are also provided for screening for compounds which activate, inhibit or potentiate cellular ion channel or receptor activity. The assays include fluorescent indicator-based assays that use viable cells containing effective levels of a fluorescent indicator which is responsive to changes in ion concentration. Activation of the ion channels or receptors is initiated in the automated measurement apparatus by injection into one or more predetermined cell-containing wells of a reagent which is a known or putative activator of the ion channels or receptors of interest. The resulting activity of the ion channels or receptors, which causes changes in ion concentration in the cytoplasm, is determined by measurement of fluorescence intensity changes of the indicator in response to an excitation wavelength.

    摘要翻译： 提供了自动化药物筛选程序的自动测量装置和方法。 该装置能够启动和测量快速或瞬时事件，例如细胞受体和/或离子通道活性。 特别地，该装置能够与预定位置对齐，一个或多个样品包含在多孔容器中，开始与试剂添加的反应并测量所得到的属性一段时间。 该装置可以在反应开始之前，期间和之后基本连续地测量和记录对应于测量属性的数据，使得可以确定快速或瞬态事件的时间过程。 在多孔容器的一个或多个孔中的反应完成之后，该装置可以将待测定的一个或多个不同孔与预定位置对准，并重复循环，直到测定预定数量的孔。 还提供了使用该装置的自动药物筛选方法，用于筛选激活，抑制或增强细胞离子通道或受体活性的化合物。 测定包括基于荧光指示剂的测定法，其使用含有有效水平的荧光指示剂的活细胞，其对离子浓度的变化有反应。 离子通道或受体的激活在自动化测量装置中通过注射到一个或多个预定的含有细胞的孔中，该试剂是已知或推定的感兴趣的离子通道或受体的激活剂。 通过测量响应于激发波长的指示剂的荧光强度变化来确定导致细胞质中离子浓度变化的离子通道或受体的所得活性。

    Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
    6.
    发明授权
    Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same 失效
    使用其自动分析设备和检测细胞表面蛋白质和/或细胞质受体功能的测定方法

    公开(公告)号：US5670113A

    公开(公告)日：1997-09-23

    申请号：US244985

    申请日：1994-06-20

    申请人： Michael Anthony Akong ,  Michael Miller Harpold ,  Gonul Velicelebi ,  Paul Brust

    发明人： Michael Anthony Akong ,  Michael Miller Harpold ,  Gonul Velicelebi ,  Paul Brust

    IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/68 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N21/64 ,  G01J3/42

    CPC分类号： G01N33/6872 ,  G01N21/6452 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058 ,  G01N35/028 ,  G01N2035/042 ,  G01N21/6428 ,  G01N2333/705 ,  G01N2500/00 ,  G01N35/109 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/11 ,  Y10T436/115831

    摘要： Automated measurement apparatus and methods for automated drug screening and for studying ion channel and cell surface receptor activity are provided. The apparatus is designed to initiate and measure rapid or transient events, such as cell receptor and/or ion channel activity. The apparatus can effect measurements of transient reactions in or more samples in a multi-well container, initiate the reaction with reagent addition and measure a resulting attribute of the sample for a period of time. The apparatus is capable of substantially continuously measuring and recording data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event is determined. Automated drug screening methods and methods for studying receptor and ion channel activity using the apparatus are also provided. The drug screening assays identify compounds that activate, inhibit or potentiate cellular ion channel or receptor activity.

    摘要翻译： PCT No.PCT / US92 / 11090 Sec。 371日期：1994年6月20日 102（e）日期1994年6月20日PCT提交1992年12月13日PCT公布。 出版物WO93 / 13423 日期1993年7月8日提供自动化药物筛选和研究离子通道和细胞表面受体活性的自动测量装置和方法。 该装置被设计为启动和测量诸如细胞受体和/或离子通道活性的快速或瞬时事件。 该装置可以实现多孔容器中或多个样品中的多个样品中的瞬态反应的测量，通过加入试剂启动反应并测量样品的结果属性一段时间。 该装置能够在反应开始之前，期间和之后基本上连续地测量和记录对应于测量属性的数据，从而确定快速或瞬态事件的时间过程。 还提供了使用该装置研究受体和离子通道活性的自动药物筛选方法和方法。 药物筛选试验鉴定激活，抑制或增强细胞离子通道或受体活性的化合物。