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    Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, asparate 101 or leucine 137
    1.
    发明授权
    Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, asparate 101 or leucine 137 有权
    使用在一种或多种位置谷氨酸盐89，天冬氨酸101或亮氨酸137突变的人碱性成纤维细胞生长因子类似物的方法

    公开(公告)号：US07138374B2

    公开(公告)日：2006-11-21

    申请号：US10785436

    申请日：2004-02-23

    申请人： Barry A. Springer ,  Michael W. Pantoliano ,  Celia M. Sharp

    发明人： Barry A. Springer ,  Michael W. Pantoliano ,  Celia M. Sharp

    IPC分类号： A61K38/18 ,  C07K14/50

    CPC分类号： C07K14/503 ,  A61K38/00

    摘要： The present invention relates to novel muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for stimulating cell division, treating a wound, treating ischemia, treating heart disease, treating neural injury, treating peripheral vascular disease, treating a gastric ulcer and treating a duodenal ulcer.

    摘要翻译： 本发明涉及具有超强调性质的人碱性成纤维细胞生长因子的新颖突变蛋白。 公开了蛋白质和各自编码的核酸物质。 本发明还体现了用于扩增所述核酸序列和产生所述突变蛋白的载体和宿主细胞。 还公开了刺激细胞分裂，治疗伤口，治疗缺血，治疗心脏病，治疗神经损伤，治疗外周血管疾病，治疗胃溃疡和治疗十二指肠溃疡的方法。

    Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
    5.
    发明授权
    Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization 有权
    用于配体开发和多变量蛋白质化学优化的微板热转移测定装置

    公开(公告)号：US06214293B1

    公开(公告)日：2001-04-10

    申请号：US09459996

    申请日：1999-12-14

    申请人： Michael W. Pantoliano ,  Roger F. Bone ,  Alexander W. Rhind ,  Francis R. Salemme

    发明人： Michael W. Pantoliano ,  Roger F. Bone ,  Alexander W. Rhind ,  Francis R. Salemme

    IPC分类号： G01N2164

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.

    摘要翻译： 本发明提供一种用于同时加热多个样品的温度调节装置和用于在样品被加热时从样品接收光谱发射的接收装置的测定装置。 在本发明的另外的方面，接收装置可以被配置为接收荧光发射，紫外光和可见光。 接收装置可以被配置为以多种方式从样本接收光谱发射，例如一次一个样本，同时来自多于一个样本，或同时来自所有样本。 温度调节装置可以配置有用于根据预定轮廓改变温度的温度控制器。

    Thermally stable serine proteases
    6.
    发明授权
    Thermally stable serine proteases 失效
    热稳定的丝氨酸蛋白酶

    公开(公告)号：US5246849A

    公开(公告)日：1993-09-21

    申请号：US671546

    申请日：1991-03-19

    申请人： Philip N. Bryan ,  Michael W. Pantoliano

    发明人： Philip N. Bryan ,  Michael W. Pantoliano

    IPC分类号： C12N9/54

    CPC分类号： C12N9/54

    摘要： The invention relates to subtilisin enzymes which have been modified by mutating a nucleotide sequence (gene) coding for the subtilisin. The modified subtilisin enzymes have enhanced thermal stability.

    摘要翻译： 本发明涉及通过突变编码枯草杆菌蛋白酶的核苷酸序列（基因）而修饰的枯草杆菌蛋白酶。 修饰的枯草杆菌蛋白酶具有增强的热稳定性。

    Method for identifying lead compounds
    8.
    发明授权
    Method for identifying lead compounds 有权
    铅化合物鉴定方法

    公开(公告)号：US06303322B1

    公开(公告)日：2001-10-16

    申请号：US09478216

    申请日：2000-01-05

    申请人： Michael W. Pantoliano ,  Alexander W. Rhind ,  Francis R. Salemme

    发明人： Michael W. Pantoliano ,  Alexander W. Rhind ,  Francis R. Salemme

    IPC分类号： G01N3353

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.

    摘要翻译： 本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到 从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与 在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

    Method for sensing and processing fluorescence data from multiple samples
    9.
    发明授权
    Method for sensing and processing fluorescence data from multiple samples 有权
    用于感测和处理来自多个样品的荧光数据的方法

    公开(公告)号：US06268218B1

    公开(公告)日：2001-07-31

    申请号：US09459997

    申请日：1999-12-14

    申请人： Michael W. Pantoliano ,  Roger F. Bone ,  Alexander W. Rhind ,  Francis R. Salemme

    发明人： Michael W. Pantoliano ,  Roger F. Bone ,  Alexander W. Rhind ,  Francis R. Salemme

    IPC分类号： G01N2164

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention provides an assay apparatus for that includes a temperature adjusting device for simultaneously heating a plurality of samples, and a receiving device for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving device can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving device can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting device can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.

    摘要翻译： 本发明提供了一种用于同时加热多个样品的温度调节装置和用于在样品被加热时从样品接收光谱发射的接收装置的测定装置。 在本发明的其它方面，接收装置可被配置为接收荧光发射，紫外光和可见光。 接收装置可以被配置为以多种方式从样本接收频谱发射，例如一次一个样本，同时来自多于一个样本，或同时来自所有样本。 温度调节装置可以配置有用于根据预定轮廓改变温度的温度控制器。

    Method for determining conditions that facilitate protein crystallization
    10.
    发明授权
    Method for determining conditions that facilitate protein crystallization 有权
    确定促进蛋白质结晶的条件的方法

    公开(公告)号：US06268158B1

    公开(公告)日：2001-07-31

    申请号：US09458663

    申请日：1999-12-10

    申请人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Francis R. Salemme ,  Barry A. Springer

    发明人： Michael W. Pantoliano ,  Eugenio C. Petrella ,  Francis R. Salemme ,  Barry A. Springer

    IPC分类号： G01N3353

    CPC分类号： C40B30/04 ,  B01L7/00 ,  B01L7/52 ,  C07K1/047 ,  C12Q1/6811 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/86 ,  G01N35/028 ,  G01N2500/00

    摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.

    摘要翻译： 本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。 该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到 从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与 在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。