公开(公告)号：US5378832A

公开(公告)日：1995-01-03

申请号：US94091

申请日：1993-07-20

申请人： Ryuichiro Kurane ,  Yasuhiro Nohata ,  Michio Shiomi ,  Shuichi Ishino ,  Akira Yotsuji ,  Hideki Murata ,  Seiji Sugimoto

发明人： Ryuichiro Kurane ,  Yasuhiro Nohata ,  Michio Shiomi ,  Shuichi Ishino ,  Akira Yotsuji ,  Hideki Murata ,  Seiji Sugimoto

IPC分类号： B01F17/56 ,  C08B37/00 ,  C12P19/04 ,  C12N1/20

CPC分类号： C12P19/04 ,  C08B37/006 ,  Y10S435/829

摘要： The present invention provides a polymer comprising a polysaccharide having excellent water absorption properties, moisture absorption properties, moisture retention properties and thickening properties. Said polysaccharide has the following properties: (A) the principal constituents of the sugar composition are rhamnose, fucose, glucose and glucuronic acid which are present in a molar ratio of (1-4):2:(1-8):(1-4); (B) elemental analysis (wt %):C: 36 .+-.3H: 7 .+-.1O: 56 .+-.4,containing 9-13% of crystalline water(C) solubility:slightly soluble in water; soluble in alkalies; insoluble in methanol, ethanol and acetone;(D) UV absorption spectrum:no absorption detected at 280 nm characteristic of proteins (peptides) or at 260 nm characteristic of nucleic acids; and(E) IR absorption spectrum:an absorption pattern characteristic of polysaccharides is observed near 800-1200.sup.-iSaid polysaccharide is produced by a fermentation method using a natural medium or a synthetic medium of Alcaligenes microorganism.

摘要翻译： PCT No.PCT / JP92 / 00695 Sec。 371日期：1993年7月20日 102（e）日期1993年7月20日PCT提交1993年7月20日PCT公布。 公开号WO93 / 11163 日期：6月10日，1993。本发明提供了包含具有优异的吸水性，吸湿性，保湿性和增稠性的多糖的聚合物。 所述多糖具有以下性质：（A）糖组合物的主要成分是以（1-4）：2：（1-8）：（1）的摩尔比存在的鼠李糖，岩藻糖，葡萄糖和葡萄糖醛酸 -4）; （B）元素分析（重量％）：C：36 +/- 3 H：7 +/- 1 O：56 +/- 4，含有9-13％的结晶水（C）溶解度：微溶于水; 溶于碱; 不溶于甲醇，乙醇和丙酮; （D）紫外线吸收光谱：蛋白质（肽）的特征性或280nm特征性的核酸在280nm处未检测到吸收; 和（E）IR吸收光谱：在800-1200-i附近观察到多糖的吸收图案特征。所述多糖通过使用天然培养基或产碱杆菌微生物的合成培养基的发酵方法产生。

公开(公告)号：US4229440A

公开(公告)日：1980-10-21

申请号：US963967

申请日：1978-11-27

申请人： Mitsugu Murofushi ,  Kuniaki Sasaki ,  Michio Shiomi ,  Yoshio Uchida ,  Tokihisa Hattori ,  Keniti Takeda

发明人： Mitsugu Murofushi ,  Kuniaki Sasaki ,  Michio Shiomi ,  Yoshio Uchida ,  Tokihisa Hattori ,  Keniti Takeda

IPC分类号： A61K31/70 ,  A61K35/66 ,  A61K31/735 ,  C08B37/00

CPC分类号： A61K31/70

摘要： KGF-C is obtained by grinding kefir grain, adding water to the ground kefir grain and heating, homogenizing the mixture and centrifuging it, adding ethanol to the obtained supernatant to obtain a precipitate, dissolving the precipitate into water, purifying the obtained liquid and freeze-drying the remaining liquid. The obtained KGF-C is soluble in water and is composed of polysaccharide as principal component and contains a very small amount of nitrogenous component, and, as sugar composition, is composed of glucose and galactose in the ratio 1:1. This KGF-C has strong antitumor action by oral administration.

摘要翻译： KGF-C通过研磨桂皮颗粒，加入水中加热，加热混合物匀浆并离心，向所得上清液中加入乙醇，得到沉淀物，将沉淀物溶解于水中，纯化所得液体并冷冻 干燥剩余的液体。 得到的KGF-C可溶于水，由多糖作为主成分，含有非常少量的含氮成分，糖组成由葡萄糖和半乳糖组成，比例为1：1。 该KGF-C通过口服给药具有强烈的抗肿瘤作用。

公开(公告)号：US08303820B2

公开(公告)日：2012-11-06

申请号：US12063318

申请日：2006-08-10

申请人： Hideki Murata ,  Tsuyoshi Mokudai ,  Michio Shiomi

发明人： Hideki Murata ,  Tsuyoshi Mokudai ,  Michio Shiomi

IPC分类号： B01D19/30 ,  B01D15/36 ,  B01J39/12 ,  C12P19/30 ,  C07H19/10

CPC分类号： C12P19/32 ,  C07H19/10

摘要： A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.

摘要翻译： 一种纯化胞苷二磷酸胆碱的方法，其包括使用H型强酸性阳离子交换树脂使含有核酸类似物的胞苷二磷酸胆碱溶液与pH值不小于0.5且不大于5.0的条件接触，并将胞苷二磷酸 用水或离子浓度不超过0.1mol / L的水溶液吸附到树脂上的胆碱分离和纯化胞苷二磷酸胆碱。

公开(公告)号：US20090286284A1

公开(公告)日：2009-11-19

申请号：US12063318

申请日：2006-08-10

申请人： Hideki Murata ,  Tsuyoshi Mokudai ,  Michio Shiomi

发明人： Hideki Murata ,  Tsuyoshi Mokudai ,  Michio Shiomi

IPC分类号： C12P19/40 ,  C07H19/048

CPC分类号： C12P19/32 ,  C07H19/10

摘要： A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.

摘要翻译： 一种纯化胞苷二磷酸胆碱的方法，其包括使用H型强酸性阳离子交换树脂使含有核酸类似物的胞苷二磷酸胆碱溶液与pH值不小于0.5且不大于5.0的条件接触，并将胞苷二磷酸 用水或离子浓度不超过0.1mol / L的水溶液吸附到树脂上的胆碱分离和纯化胞苷二磷酸胆碱。

公开(公告)号：US5180670A

公开(公告)日：1993-01-19

申请号：US629453

申请日：1990-12-18

申请人： Satoshi Iwata ,  Michio Shiomi

发明人： Satoshi Iwata ,  Michio Shiomi

IPC分类号： B01D15/08 ,  B01D15/32 ,  C07D487/14 ,  C12P17/18

CPC分类号： C07D487/14 ,  B01D15/325 ,  C12P17/182

摘要： A method for purification of mitomycin C comprises;a. a step of treating the culture of a mitomycin C-producing microorganism with reverse phase adsorption resin to adsorb mitomycin C onto the resin;b. a step of eluting mitomycin C with ethyl acetate out of the resin;c. a step of adding phosphate buffer to the eluate and then evaporating ethyl acetate;d. a step of passing, under pressure, the residue remaining after the evaporation through a column packed with a reverse phase adsorbent having a small particle diameter to adsorb mitomycin C onto the resin;e. a step of eluting mitomycin C with aqueous methanol out of the resin under pressure;f. a step of treating the eluate with a reverse phase adsorption resin to adsorb mitomycin C onto the resin;g. a step of eluting mitomycin C with methanol out of the resin; and,h. a step of concentrating the methanol eluate to crystallize mitomycin C.By this method, mitomycin C can be efficiently purified in an industrial scale.

摘要翻译： 丝裂霉素C的纯化方法包括： 一个。 用反相吸附树脂处理丝裂霉素C-生产微生物的培养物以将丝裂霉素C吸附到树脂上的步骤; b。 用乙酸乙酯从树脂中洗脱丝裂霉素C的步骤; C。 向洗脱液中加入磷酸盐缓冲液，然后蒸发乙酸乙酯的步骤; d。 在压力下通过填充有粒径小的反相吸附剂的柱子在蒸发后残留的残余物吸附丝素霉素C到树脂上的步骤; e。 用甲醇水溶液将丝裂霉素C从压力下挤出树脂的步骤; F。 用反相吸附树脂处理洗脱液以将丝裂霉素C吸附到树脂上的步骤; G。 用甲醇从树脂中洗脱丝裂霉素C的步骤; 和，h。 浓缩甲醇洗脱液以结晶丝裂霉素C的步骤。通过这种方法，可以以工业规模有效地纯化丝裂霉素C.

公开(公告)号：US5364775A

公开(公告)日：1994-11-15

申请号：US972824

申请日：1992-11-06

申请人： Ryoichi Katsumata ,  Takashi Ohshiro ,  Haruhiko Yokoi ,  Michio Shiomi

发明人： Ryoichi Katsumata ,  Takashi Ohshiro ,  Haruhiko Yokoi ,  Michio Shiomi

IPC分类号： C12P13/04 ,  C12P13/24 ,  C12R1/01 ,  C12R1/37

CPC分类号： C12P13/24 ,  C12P13/04 ,  Y10S435/822 ,  Y10S435/873

摘要： The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture.The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.

摘要翻译： 本发明提供了生产反式-L-羟基脯氨酸的方法，该方法包括培养能够分解反式-L-羟基脯氨酸以外的氨基酸但基本上不能分解反式-L-羟脯氨酸的微生物，该培养基含有 胶原水解产物，并从所得培养物中回收反式-L-羟脯氨酸。 该方法基于胶原蛋白水解产物中所含的氨基酸的总重量，提高了反式-L-羟脯氨酸的含量，能有效地制备可用作药物合成起始原料的反式-L-羟脯氨酸。

公开(公告)号：US5227007A

公开(公告)日：1993-07-13

申请号：US767904

申请日：1991-09-30

申请人： Tadao Tateba ,  Michio Shiomi

发明人： Tadao Tateba ,  Michio Shiomi

IPC分类号： C07C227/42

CPC分类号： C07C227/42

摘要： Disclosed is a process for producing crystals of a salt of an acidic amino acid and a basic amino acid, which comprises adding a water-miscible solvent to an aqueous solution of the salt to prepare a solution 90% or more saturated or supersaturated with the salt, heating the resultant solution under reflux until the formation of crystals stops, adding gradually a water-miscible solvent to the solution under reflux to form crystals of the salt, and recovering the crystals from the resultant mixture.

摘要翻译： 公开了一种生产酸性氨基酸和碱性氨基酸盐的晶体的方法，该方法包括向盐水溶液中加入水混溶性溶剂以制备90％以上饱和或过饱和盐的溶液 在回流下加热得到的溶​​液直至形成晶体停止，在回流下逐渐加入水溶性溶剂，形成盐晶体，并从所得混合物中回收晶体。