Method for expressing and purifying proteins using budded baculovirus
    3.
    发明授权
    Method for expressing and purifying proteins using budded baculovirus 失效
    用芽状杆状病毒表达和纯化蛋白质的方法

    公开(公告)号:US07070978B2

    公开(公告)日:2006-07-04

    申请号:US09884481

    申请日:2001-06-20

    IPC分类号: C12N7/02 C12N15/86 C12N15/09

    摘要: Method for efficiently expressing a protein in an active form by using baculovirus expression system wherein the protein is selected from a membrane-bound enzyme, a substrate of the membrane-bound enzyme, a membrane-bound enzyme activator, a membrane-bound transport protein, a channel protein, a membrane structural protein, a protein involved in adhesion, a protein involved in antigen presentation, or a protein involved in formation of high dimensional structure of a protein. There is provided a method for expressing such a protein by culturing a host infected with at least one type of recombinant baculovirus which contains a gene encoding the protein, wherein the protein is expressed in a budded baculovirus released from the host.

    摘要翻译: 通过使用杆状病毒表达系统有效地表达活性形式的蛋白质的方法,其中所述蛋白质选自膜结合酶,膜结合酶的底物,膜结合酶活化剂,膜结合的转运蛋白, 通道蛋白,膜结构蛋白,参与粘附的蛋白质,参与抗原呈递的蛋白质或涉及蛋白质高维结构形成的蛋白质。 提供了通过培养感染有至少一种类型的重组杆状病毒的宿主来表达这种蛋白质的方法,该重组杆状病毒含有编码该蛋白质的基因,其中该蛋白质在从该宿主释放的芽状杆状病毒中表达。