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    PROMOTION OF SOMATIC EMBRYOGENESIS IN PLANTS BY WUSCHEL GENE EXPRESSION
    2.
    发明申请
    PROMOTION OF SOMATIC EMBRYOGENESIS IN PLANTS BY WUSCHEL GENE EXPRESSION 有权
    通过WUSCHEL基因表达促进植物中的生物发育

    公开(公告)号：US20120102594A1

    公开(公告)日：2012-04-26

    申请号：US13347839

    申请日：2012-01-11

    申请人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

    发明人： Jianru ZUO ,  Qi-Wen NIU ,  Giovanna FRUGIS ,  Nam-Hai CHUA

    IPC分类号： A01H1/06

    CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

    摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

    摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

    FUNCTIONAL ANALYSIS OF JATROPHA CURCAS GENES
    3.
    发明申请
    FUNCTIONAL ANALYSIS OF JATROPHA CURCAS GENES 有权
    JATROPHA CURCAS基因的功能分析

    公开(公告)号：US20110265223A1

    公开(公告)日：2011-10-27

    申请号：US13141752

    申请日：2009-12-16

    申请人： Jian Ye ,  Nam Hai Chua ,  JIng Qu

    发明人： Jian Ye ,  Nam Hai Chua ,  JIng Qu

    IPC分类号： A01H3/00 ,  C12Q1/68

    CPC分类号： C12N15/8218

    摘要： The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).

    摘要翻译： 本发明涉及基因组尺度上的麻疯树基因的功能分析领域。 更具体地，本发明涉及使用病毒诱导的基因沉默在基因组规模上高麻醉麻疯树基因的高通量功能分析的方法。 该方法涉及使用烟草拨浪鼓病毒（TRV）。

    Promotion of somatic embryogenesis in plants by Wuschel gene expression
    4.
    发明授权
    Promotion of somatic embryogenesis in plants by Wuschel gene expression 有权
    通过Wuschel基因表达促进植物体细胞胚发生

    公开(公告)号：US07816580B2

    公开(公告)日：2010-10-19

    申请号：US12722981

    申请日：2010-03-12

    申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

    发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

    摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

    摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

    SELECTION METHOD
    5.
    发明申请
    SELECTION METHOD 审中-公开
    选择方法

    公开(公告)号：US20100186125A1

    公开(公告)日：2010-07-22

    申请号：US12448263

    申请日：2007-12-14

    申请人： Simon Moller ,  Nam-Hai Chua

    发明人： Simon Moller ,  Nam-Hai Chua

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10

    CPC分类号： C12N15/8214

    摘要： The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.

    摘要翻译： 本发明涉及一种生产转化植物细胞的方法。 更具体地，该方法涉及用靶向植物质体的转化盒转化植物细胞，其包含选择基因，例如异戊烯基转移酶（IPT）和转基因。 在选择转化质体后，在植物细胞中诱导重组酶的表达，这导致选择基因从质体中切除和转化子在质体中的表达。 本发明还提供包含转化盒的细胞和植物。

    ESR2 - a plant gene that can promote shoot regeneration
    6.
    发明授权
    ESR2 - a plant gene that can promote shoot regeneration 失效
    ESR2 - 可以促进苗再生的植物基因

    公开(公告)号：US06441276B1

    公开(公告)日：2002-08-27

    申请号：US09964850

    申请日：2001-09-28

    申请人： Yoshihisa Ikeda ,  Nam-Hai Chua

    发明人： Yoshihisa Ikeda ,  Nam-Hai Chua

    IPC分类号： C12N1587

    CPC分类号： C12N15/821 ,  C07K14/415 ,  C12N15/8295

    摘要： A plant gene, Esr2, has been found which when overexpressed in plant cells results in cells which have cytokinin-independent cell growth. This feature allows the encoded protein ESR2 to be used as a selectable marker of transformed cells by growing the transformed cells on cytokinin-free media. It has also been found that overexpression of ESR2 in cells grown in the presence of cytokinins results in a higher regeneration of plants. This feature allows the gene to be used to obtain greater regeneration of plant cells.

    摘要翻译： 已经发现植物基因Esr2在植物细胞中过表达时产生具有细胞分裂素依赖性细胞生长的细胞。 该特征允许编码的蛋白质ESR2通过在不含细胞分裂素的培养基上生长转化的细胞而用作转化细胞的选择标记。 还已经发现，在细胞分裂素存在下生长的细胞中ESR2的过表达导致植物更高的再生。 该特征允许该基因用于获得更大的植物细胞再生。

    Alteration of plant morphology by control of profilin expression
    7.
    发明授权
    Alteration of plant morphology by control of profilin expression 失效
    通过控制profilin表达改变植物形态

    公开(公告)号：US06344601B1

    公开(公告)日：2002-02-05

    申请号：US09061897

    申请日：1998-04-17

    申请人： Nam-Hai Chua ,  Srinivasan Ramachandran ,  Hans Erik Molager Christensen

    发明人： Nam-Hai Chua ,  Srinivasan Ramachandran ,  Hans Erik Molager Christensen

    IPC分类号： C12N1582

    CPC分类号： C12N15/827 ,  C12N15/8261 ,  Y02A40/146

    摘要： Plant growth habit is altered by causing either under-expression or over-expression of profilin in a plant cell. Under-expression of profilin can be achieved by transforming a plant or plant cell with a gene expressing an antisense mRNA complementary to the mRNA transcribed by the coding sequence of a profilin gene and expressing the gene in the plant or plant cell such that the antisense mRNA inhibits the production of the profilin in the plant or plant cell. Under-expression of profilins in plants can lead to such alterations in growth habit as a dwarf phenotype, a reduced root and root hair system, and delay in the onset of flowering. Over-expression of profilin can be achieved by transforming a plant or plant cell with a gene capable of expressing a profilin in the plant or plant cell and causing the transformed gene to be expressed in the plant or plant cell. Over-expression of profilin in a plant can lead to such alterations in growth habit as a tall phenotype, an expansion of the root and root hair system, expansion of leaf surface area and accelerating the onset of flowering.

    摘要翻译： 通过在植物细胞中引起profilin的低表达或过度表达来改变植物生长习性。 profilin的低表达可以通过用表达与由profilin基因的编码序列转录的mRNA互补的反义mRNA的基因转化植物或植物细胞并在植物或植物细胞中表达该基因来实现，使得反义mRNA 抑制植物细胞或植物细胞中profilin的产生。 植物中profilins的低表达可导致生长习性如矮矮化表型，根系和根系减少延迟以及开花发生的延迟。 通过用能够在植物或植物细胞中表达profilin的基因转化植物或植物细胞并使转化的基因在植物或植物细胞中表达，可以实现profilin的过表达。 植物中profilin的过度表达可导致生长习性如高表型，根系和根系发育扩张，叶表面积扩大和加速开花的发生。

    cDNA encoding a polypeptide including a hev ein sequence
    8.
    发明授权
    cDNA encoding a polypeptide including a hev ein sequence 失效
    编码包含血红素序列的多肽的cDNA

    公开(公告)号：US6083687A

    公开(公告)日：2000-07-04

    申请号：US888367

    申请日：1992-05-26

    申请人： Natasha V. Raikhel ,  Willem F. Broekaert ,  Nam-Hai Chua ,  Anil Kush

    发明人： Natasha V. Raikhel ,  Willem F. Broekaert ,  Nam-Hai Chua ,  Anil Kush

    IPC分类号： C07K14/415 ,  C12Q1/68

    CPC分类号： C07K14/415 ,  C07K2319/02

    摘要： A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

    摘要翻译： 通过聚合酶链反应（PCR），使用对应于作为引物的两个区域的混合寡核苷酸和作为模板的三叶草胶体cDNA文库，分离编码水解蛋白的cDNA克隆（HEV1）。 HEV1长1018个核苷酸，包括204个氨基酸的开放阅读框。 推测的氨基酸序列含有17个氨基酸残基的随机信号序列，随后是187个氨基酸的多肽。 氨基末端区域（43个氨基酸）与外源蛋白相同，并显示与几丁质结合蛋白质和马铃薯和杨树中伤口诱导基因的氨基末端的同源性。 多肽的羧基末端部分（144个氨基酸）与马铃薯的伤口诱导基因的羧基末端区域同源74-79％。 伤口以及植物激素脱落酸和乙烯的应用导致叶片，茎和胶乳中hevein转录物的积累，但不能在根中积累，如使用cDNA作为探针所示。 在本发明的蛋白质的大肠杆菌中产生融合蛋白，由大肠杆菌产生的麦芽糖结合蛋白。