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公开(公告)号：US08486667B2
公开(公告)日：2013-07-16
申请号：US13286955
申请日：2011-11-01
申请人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
CPC分类号： C12N9/2437 , C11D3/38 , C11D3/38636 , C11D3/38645 , C12N9/2445 , C12N15/1137 , C12N2310/11 , C12P7/10 , C12P7/14 , C12P19/02 , C12P19/14 , C12P2201/00 , C12Y302/01004 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
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公开(公告)号：US08298802B2
公开(公告)日：2012-10-30
申请号：US13102298
申请日：2011-05-06
申请人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
CPC分类号： A21D8/042 , C11D3/38645 , C12N9/2445 , C12N15/1137 , C12Y302/01021 , Y02E50/17
摘要： The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.
摘要翻译： 本发明提供了一种命名为bgl3和相应的BGL3氨基酸序列的新颖的 - 葡糖苷酶核酸序列。 本发明还提供了包含编码BGL3的核酸序列，重组BGL3蛋白的表达载体和宿主细胞及其制备方法。
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3.发明申请Expression Of Granular Starch Hydrolyzing Enzymes In Trichoderma And Process For Producing Glucose From Granular Starch Substrates 有权颗粒状淀粉水解酶在木霉菌中的表达及从颗粒状淀粉底物生产葡萄糖的方法公开(公告)号：US20120156329A1
公开(公告)日：2012-06-21
申请号：US13230707
申请日：2011-09-12
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, JR. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, JR. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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4.发明授权公开(公告)号：US08138321B2
公开(公告)日：2012-03-20
申请号：US12441574
申请日：2007-09-21
CPC分类号： C12N9/88 , C12N15/80 , C12Y202/01006
摘要： A nucleic acid encoding an acetolactate synthase (ALS) protein that provides resistance to ALS inhibitors, e.g., sulphonylurea and imidazolinone compounds, is provided. The nucleic acid may be used as a selectable marker for expression of a protein of interest in host cells.
摘要翻译： 提供编码提供对ALS抑制剂（例如磺酰脲和咪唑啉酮化合物）的抗性的乙酰乳酸合酶（ALS）蛋白质的核酸。 核酸可以用作在宿主细胞中表达目标蛋白质的选择性标记物。
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5.发明申请公开(公告)号：US20120052551A1
公开(公告)日：2012-03-01
申请号：US13225266
申请日：2011-09-02
CPC分类号： C12N9/242 , C12N9/2417
摘要： The present invention relates to a polypeptide having alpha-amylase activity obtained from a strain of Aspergillus niger.
摘要翻译： 本发明涉及从黑曲霉菌株获得的具有α-淀粉酶活性的多肽。
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公开(公告)号：US20110165288A1
公开(公告)日：2011-07-07
申请号：US10587006
申请日：2005-02-17
申请人： Gang Duan , Nigel Dunn-Coleman , Jayarama K. Shetty , Alex Sung , Ying Qian
发明人： Gang Duan , Nigel Dunn-Coleman , Jayarama K. Shetty , Alex Sung , Ying Qian
CPC分类号： A23J1/125 , A23J3/346 , A23K20/147 , A23L7/107 , A23L7/198
摘要： The present invention relates to a process for producing a rice protein concentrate from a rice substrate comprising, enzymatically hydrolyzing a rice substrate with an enzyme having granular starch hydrolyzing (GSH) activity and a second starch hydrolyzing enzyme at a temperature at or below 72° C. and at a pH of about 3.0 to 6.5 to obtain a solubilized starch fraction and a residue fraction which includes insoluble protein, and separating the solubilized starch fraction from the residue to obtain a rice protein concentrate. The rice protein concentrate may be used in animal feed and human food formulations.
摘要翻译： 本发明涉及一种从水稻基质制备大米蛋白浓缩物的方法，包括在72℃或低于72℃的温度下，用具有颗粒状淀粉水解（GSH）活性的酶和第二淀粉水解酶酶水解水稻基质 并且在约3.0至6.5的pH下，得到溶解的淀粉级分和包含不溶性蛋白质的残余物级分，并将残留的溶解的淀粉部分分离，得到大米蛋白质浓缩物。 米蛋白浓缩物可用于动物饲料和人类食物制剂中。
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公开(公告)号：US20110086410A1
公开(公告)日：2011-04-14
申请号：US11938932
申请日：2007-11-13
申请人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
发明人： Nigel Dunn-Coleman , Frits Goedegebuur , Michael Ward , Jian Yao
IPC分类号： C12N9/42
CPC分类号： C12N9/2445 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.
摘要翻译： 本发明提供了命名为bgl4和相应的BGL4氨基酸序列的新颖的 - 葡糖苷酶核酸序列。 本发明还提供了包含编码BGL4的核酸序列，重组BGL4蛋白的表达载体和宿主细胞及其制备方法。
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8.再颁专利Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis 有权河曲酸稳定α淀粉酶的异源表达及其在颗粒淀粉水解中的应用公开(公告)号：USRE42046E1
公开(公告)日：2011-01-18
申请号：US12257332
申请日：2008-10-23
申请人： Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty
发明人： Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty
CPC分类号： C12P7/06 , C12N9/242 , C12N9/2428 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25-40° C. to obtain a fermentation broth having 5 to 20% ethanol.
摘要翻译： 本发明涉及含有具有淀粉淀粉水解活性的酸稳定性α淀粉酶（asAA）的粒状淀粉水解酶组合物。 本发明还涉及一种生产醇的一步法，该方法包括在发酵步骤中使颗粒淀粉基质与具有颗粒状淀粉水解（GSH）活性的酸稳定性α淀粉酶（asAA）和葡糖淀粉酶（GA）接触， 还在25-40℃的温度下包含产乙醇微生物以获得具有5至20％乙醇的发酵液。
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9.发明申请公开(公告)号：US20100273201A1
公开(公告)日：2010-10-28
申请号：US12441574
申请日：2007-09-21
CPC分类号： C12N9/88 , C12N15/80 , C12Y202/01006
摘要： A nucleic acid encoding an acetolactate synthase (ALS) protein that provides resistance to ALS inhibitors, e.g., sulphonylurea and imidazolinone compounds, is provided. The nucleic acid may be used as a selectable marker for expression of a protein of interest in host cells.
摘要翻译： 提供编码提供对ALS抑制剂（例如磺酰脲和咪唑啉酮化合物）的抗性的乙酰乳酸合酶（ALS）蛋白质的核酸。 核酸可以用作在宿主细胞中表达目标蛋白质的选择性标记物。
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公开(公告)号：US20090317872A1
公开(公告)日：2009-12-24
申请号：US12485767
申请日：2009-06-16
CPC分类号： C12N9/58 , A23L13/424 , A23L33/18 , C12N9/48 , C12P7/06 , C12P7/10 , C12P19/02 , Y02E50/16 , Y02E50/17
摘要： The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
摘要翻译： 本发明涉及生产乙醇的方法，并且在无烹饪发酵中减少残余淀粉的产生，包括在合适的发酵条件下，在低于淀粉糊化温度的温度下，使含有颗粒淀粉的底物与颗粒状淀粉水解酶，蛋白酶和发酵微生物接触 淀粉底物的温度以产生乙醇，其中乙醇生产增加，并且残余淀粉的量与基本上相似的不含蛋白酶的方法相比降低。
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