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    Methods of Screening an Agent for an Activity in an Isolated Eye of a Teleost
    1.
    发明申请
    Methods of Screening an Agent for an Activity in an Isolated Eye of a Teleost 失效
    在Teleost的隔离眼中筛选活动剂的方法

    公开(公告)号：US20110218119A1

    公开(公告)日：2011-09-08

    申请号：US13037104

    申请日：2011-02-28

    申请人： Patricia McGrath ,  Wen Lin Seng

    发明人： Patricia McGrath ,  Wen Lin Seng

    IPC分类号： C40B30/06 ,  C12Q1/02 ,  C12Q1/37 ,  C12Q1/34 ,  C12Q1/68 ,  G01N33/567

    CPC分类号： G01N33/5088 ,  A61K49/0008 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/6876

    摘要： The present invention provides methods of screening an agent for an activity in an isolated organ, e.g., eye, from a teleost, e.g., zebrafish. Methods of isolating eyes from zebrafish are provided. Methods of screening an agent for an ocular activity in the isolated eye are provided. Methods of screening an agent for an ocular activity in a model of ocular disease or disorder are provided. Methods of screening an agent for an ocular activity in the isolated eye and for screening the agent for cell death and/or toxic activity in the eye or other organ or tissue are provided. The invention further provides high throughput methods of screening agents for an activity in isolated eyes of zebrafish in multi-well plates.

    摘要翻译： 本发明提供筛选来自硬骨鱼例如斑马鱼的分离的器官（例如眼睛）中的活性物质的方法。 提供了从斑马鱼分离眼睛的方法。 提供了筛选分离眼中眼部活性物质的方法。 提供了在眼部疾病或病症模型中筛选眼睛活性物质的方法。 提供了在眼睛或其他器官或组织中筛选隔离眼睛中的眼部活性物质和用于筛选细胞死亡和/或毒性活性的试剂的方法。 本发明还提供了多孔板中斑马鱼孤立眼中活性筛选剂的高通量方法。

    GEL MICRODROPS IN GENETIC ANALYSIS
    3.
    发明申请
    GEL MICRODROPS IN GENETIC ANALYSIS 审中-公开

    公开(公告)号：US20080160498A1

    公开(公告)日：2008-07-03

    申请号：US11868139

    申请日：2007-10-05

    申请人： Jan Trnovsky ,  Patricia McGrath

    发明人： Jan Trnovsky ,  Patricia McGrath

    IPC分类号： A01N1/02 ,  C12Q1/68 ,  C12N5/00 ,  C12Q1/70

    CPC分类号： C12Q1/6834 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/689 ,  C12Q1/70 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2523/101 ,  C12Q2565/501 ,  C12Q2563/161

    摘要： The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.

    摘要翻译： 本发明提供核酸分析方法。 这样的方法需要形成包封生物实体群体的凝胶微滴群，每个实体包含核酸，其中群体中的至少一些微滴包封单个实体。 然后将凝胶微滴的群体与探针接触，其中探针与至少一种凝胶微滴中核酸中的至少一个互补序列特异性杂交。 然后分析或检测至少一个凝胶微滴。 生物实体可以是细胞，病毒，细胞核和染色体。

    METHODS FOR INTRODUCING HETEROLOGOUS CELLS INTO FISH
    4.
    发明申请
    METHODS FOR INTRODUCING HETEROLOGOUS CELLS INTO FISH 审中-公开
    将异位细胞引入鱼类的方法

    公开(公告)号：US20110286973A1

    公开(公告)日：2011-11-24

    申请号：US12951767

    申请日：2010-11-22

    申请人： George N. Serbedzija ,  Patricia McGrath

    发明人： George N. Serbedzija ,  Patricia McGrath

    IPC分类号： A61K35/12 ,  A01K67/027 ,  A61P35/00 ,  C12Q1/70 ,  C40B30/04 ,  G01N33/574 ,  G01N33/53

    CPC分类号： A61K49/0058 ,  A01K67/0271 ,  A01K2227/40 ,  A61K49/00 ,  A61K49/0008 ,  A61K49/0032 ,  A61K49/0043 ,  C12Q1/68 ,  G01N33/5082 ,  G01N33/5088 ,  G01N2333/4603

    摘要： The invention provides methods of introducing heterologous cells into fish. After introduction cells remain viable, and in some instances proliferate, for sufficient time to conduct a variety of analyses on the heterologous cells or the fish or both. Such methods are useful for screening potential drugs for toxicity toward introduced cells or for capacity to stimulate differentiation and/or proliferation of introduced cells. Such methods are also useful for diagnosing the presence of small quantities of cancerous cells or pathogens in patient tissue samples. Such methods are also useful for culturing cells for subsequent use in cell or tissue engineering.

    摘要翻译： 本发明提供了将异源细胞引入鱼中的方法。 在引入细胞后，细胞保持活力，并且在一些情况下增殖，足够的时间对异源细胞或鱼或两者进行各种分析。 这些方法可用于筛选潜在药物对引入细胞的毒性或刺激引入细胞分化和/或增殖的能力。 这样的方法也可用于诊断患者组织样品中少量癌细胞或病原体的存在。 这样的方法也可用于培养细胞以用于细胞或组织工程中。

    Methods of screening an agent for an activity in an isolated eye of a teleost
    6.
    发明申请
    Methods of screening an agent for an activity in an isolated eye of a teleost 有权
    筛选在硬骨鱼孤立眼中活动的药剂的方法

    公开(公告)号：US20090010849A1

    公开(公告)日：2009-01-08

    申请号：US12137509

    申请日：2008-06-11

    申请人： Patricia McGrath ,  Wen Lin Seng

    发明人： Patricia McGrath ,  Wen Lin Seng

    IPC分类号： A61K49/00

    CPC分类号： G01N33/5088 ,  A61K49/0008 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/6876

    摘要： The present invention provides methods of screening an agent for an activity in an isolated organ, e.g., eye, from a teleost, e.g., zebrafish. Methods of isolating eyes from zebrafish are provided. Methods of screening an agent for an ocular activity in the isolated eye are provided. Methods of screening an agent for an ocular activity in a model of ocular disease or disorder are provided. Methods of screening an agent for an ocular activity in the isolated eye and for screening the agent for cell death and/or toxic activity in the eye or other organ or tissue are provided. The invention further provides high throughput methods of screening agents for an activity in isolated eyes of zebrafish in multi-well plates.

    摘要翻译： 本发明提供筛选来自硬骨鱼例如斑马鱼的分离的器官（例如眼睛）中的活性物质的方法。 提供了从斑马鱼分离眼睛的方法。 提供了筛选分离眼中眼部活性物质的方法。 提供了在眼部疾病或病症模型中筛选眼睛活性物质的方法。 提供了在眼睛或其他器官或组织中筛选隔离眼睛中的眼部活性物质和用于筛选细胞死亡和/或毒性活性的试剂的方法。 本发明还提供了多孔板中斑马鱼孤立眼中活性筛选剂的高通量方法。

    Secretions of proteins by encapsulated cells
    7.
    发明授权
    Secretions of proteins by encapsulated cells 失效
    通过封装细胞分泌蛋白质

    公开(公告)号：US06806058B2

    公开(公告)日：2004-10-19

    申请号：US10157351

    申请日：2002-05-28

    申请人： Diana Jesperson ,  Yevgenya Akselband ,  Patricia McGrath ,  Jan Trnovsky ,  Phillip T. Moen

    发明人： Diana Jesperson ,  Yevgenya Akselband ,  Patricia McGrath ,  Jan Trnovsky ,  Phillip T. Moen

    IPC分类号： G01N3353

    CPC分类号： G01N33/5432 ,  C07K16/00 ,  C07K2317/52 ,  G01N33/5436 ,  G01N33/56966 ,  G01N33/56972 ,  Y10S436/823

    摘要： The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.

    摘要翻译： 本发明提供了从包封在微滴中的细胞中分泌分泌蛋白质的方法。 与以前的制剂相比，微滴与生物素基质分子配制，生物素与基质分子的比例降低。 减少的比例对于改善检测的分辨率是有利的，并且允许同时检测多种分泌蛋白质和/或多种细胞表面标志物。 本发明进一步提供了分离从IgM同种型转换的IgG同种型抗体的方法。

    METHOD AND DEVICE FOR REMOVING PARTICLES FROM LIQUID AND PLACING THEM INTO A HOLDING DEVICE
    8.
    发明申请
    METHOD AND DEVICE FOR REMOVING PARTICLES FROM LIQUID AND PLACING THEM INTO A HOLDING DEVICE 审中-公开
    从液体中取出颗粒并将其放置在保持装置中的方法和装置

    公开(公告)号：US20140234959A1

    公开(公告)日：2014-08-21

    申请号：US14115705

    申请日：2012-05-04

    申请人： Patricia McGrath ,  Edward O'Lear ,  Wen Lin Seng ,  Demian Park ,  Brandon Stafford

    发明人： Patricia McGrath ,  Edward O'Lear ,  Wen Lin Seng ,  Demian Park ,  Brandon Stafford

    IPC分类号： G01N1/08

    CPC分类号： G01N1/08 ,  B01L3/021 ,  B01L3/0289 ,  B01L2200/0647 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N35/1065

    摘要： Methods, devices, and systems are disclosed for collecting or gathering samples, such as zebrafish or zebrafish eggs or embryos, dispersed in a liquid and transferring the collected or gathered samples into discrete receptacles of a holding fixture (e.g. the wells of a multi-well plate, a Petri dish, or any other suitable fixture having one or more receptacles). A method includes drawing some of the liquid through a plurality of openings in a retention structure so as to draw at least some of the samples into contact with associated openings, retaining the samples in contact with the retention structure adjacent to the associated openings using differential pressure, moving at least one of the retention structure or a holding fixture relative to the other while the samples are retained adjacent the associated openings, and ejecting samples from the retention structure into associated discrete receptacles of the holding fixture.

    摘要翻译： 公开了用于收集或收集分散在液体中的诸如斑马鱼或斑马鱼卵或胚胎的样品的方法，装置和系统，并将收集或收集的样品转移到保持夹具的离散容器（例如多孔的孔中） 板，培养皿或具有一个或多个容器的任何其它合适的夹具）。 一种方法包括通过保持结构中的多个开口抽取一些液体，以便使至少一些样品与相关联的开口相接触，使用压差保持样品与邻近相关开口的保持结构接触 当所述样本保持在相关联的开口附近时，移动所述保持结构或保持夹具中的至少一个，并将样品从所述保持结构喷射到所述保持夹具的相关联的分离的容器中。

    Methods of screening an agent for an activity in an isolated eye of a teleost
    9.
    发明授权
    Methods of screening an agent for an activity in an isolated eye of a teleost 失效

    公开(公告)号：US08367364B2

    公开(公告)日：2013-02-05

    申请号：US13037104

    申请日：2011-02-28

    申请人： Patricia McGrath ,  Wen Lin Seng

    发明人： Patricia McGrath ,  Wen Lin Seng

    IPC分类号： C12Q1/34

    CPC分类号： G01N33/5088 ,  A61K49/0008 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/6876

    摘要： The present invention provides methods of screening an agent for an activity in an isolated organ, e.g., eye, from a teleost, e.g., zebrafish. Methods of isolating eyes from zebrafish are provided. Methods of screening an agent for an ocular activity in the isolated eye are provided. Methods of screening an agent for an ocular activity in a model of ocular disease or disorder are provided. Methods of screening an agent for an ocular activity in the isolated eye and for screening the agent for cell death and/or toxic activity in the eye or other organ or tissue are provided. The invention further provides high throughput methods of screening agents for an activity in isolated eyes of zebrafish in multi-well plates.