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公开(公告)号：US08367377B2
公开(公告)日：2013-02-05
申请号：US12618991
申请日：2009-11-16
CPC分类号： C12Q1/68 , B01J19/0046 , B01J2219/00432 , B01J2219/00436 , B01J2219/00497 , B01J2219/005 , B01J2219/00511 , B01J2219/00527 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00707 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C12Q1/6874 , C12Q2549/125
摘要： Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid.
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公开(公告)号：US07635562B2
公开(公告)日：2009-12-22
申请号：US11137928
申请日：2005-05-25
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , B01J19/0046 , B01J2219/00432 , B01J2219/00436 , B01J2219/00497 , B01J2219/005 , B01J2219/00511 , B01J2219/00527 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00707 , B01J2219/00722 , B82Y30/00 , C12Q1/6837 , C12Q1/6874 , C12Q2549/125
摘要： Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid.
摘要翻译： 本发明的方法包括用于核酸序列测定的方法和装置。 通常，本发明涉及制备用于测序靶核酸的底物。
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公开(公告)号：US20090061439A1
公开(公告)日：2009-03-05
申请号：US12114163
申请日：2008-05-02
申请人： Philip Richard Buzby
发明人： Philip Richard Buzby
CPC分类号： C12Q1/6869 , C12Q2565/102 , C12Q2533/101
摘要： The invention provides nucleotide analogs and methods of using them in sequencing reactions.
摘要翻译： 本发明提供核苷酸类似物和在测序反应中使用它们的方法。
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公开(公告)号：US20080076123A1
公开(公告)日：2008-03-27
申请号：US11528959
申请日：2006-09-27
申请人： Philip Richard Buzby
发明人： Philip Richard Buzby
CPC分类号： C12Y207/07007 , C12N9/1252
摘要： Described herein is a mutant polymerase, specifically the Klenow exo− polymerase in which the proline at amino acid position 680 is replaced by a glycine (P680G), and its use in sequencing a single polynucleotide template using single molecule sequencing.
摘要翻译： 本文描述的是突变型聚合酶，特别是其中氨基酸位置680处的脯氨酸被甘氨酸（P680G）替代的Klenow外切聚合酶，并且其用于使用单分子测序单个多核苷酸模板 测序
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公开(公告)号：US07220549B2
公开(公告)日：2007-05-22
申请号：US11027165
申请日：2004-12-30
申请人： Philip Richard Buzby
发明人： Philip Richard Buzby
CPC分类号： C12Q1/68 , C12Q1/6834 , C12Q1/6869 , C12Q2563/131 , C12Q2533/101 , C12Q2535/101
摘要： The invention provides methods for sequencing a nucleic acid comprising stabilizing a primer/target nucleic acid duplex on a substrate. Methods of the invention generally contemplate the use of a dual-anchored primer/target nucleic acid duplex, or a stabilizing molecule in a single molecule sequencing reaction.
摘要翻译： 本发明提供了对核酸进行测序的方法，包括稳定底物上的引物/靶核酸双链体。 本发明的方法通常考虑在单分子测序反应中使用双锚定引物/靶核酸双链体或稳定分子。
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公开(公告)号：US06437141B2
公开(公告)日：2002-08-20
申请号：US09824316
申请日：2001-04-02
申请人： Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人： Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号： C07D27904
CPC分类号： A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要： A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
摘要翻译： 具有化学式R 1 -R 8的花青染料各自独立地选自氢，C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇，胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。
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公开(公告)号：US06224644B1
公开(公告)日：2001-05-01
申请号：US09448242
申请日：1999-11-24
申请人： Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人： Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号： C07D27904
CPC分类号： A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要： A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
摘要翻译： 具有化学式R 1 -R 8的花青染料各自独立地选自氢，C 1 -C 6烷基和在其上具有亲水取代基的C 0 -C 4烷基。 R11和R12被选择为包括能够通过亲核置换机理与靶分子反应的游离或保护的硫醇，胺或羟基取代基。 该染料可用于标记各种靶分子。 描述了合成合适的杂环和吲哚衍生物作为前述花青染料的前体的方法。
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公开(公告)号：US07169560B2
公开(公告)日：2007-01-30
申请号：US10852482
申请日：2004-05-24
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2527/113
摘要： The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
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公开(公告)号：US06197956B1
公开(公告)日：2001-03-06
申请号：US09449333
申请日：1999-11-24
申请人： Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
发明人： Malcolm Harry Randall , Philip Richard Buzby , Thomas Joseph Erickson , Joseph David Trometer , Joseph John Miller, Jr. , David George Ahern , Mark Norman Bobrow
IPC分类号： C07D27916
CPC分类号： A61K49/0021 , A61K49/0032 , A61K49/0052 , A61K49/0056 , C07D209/08
摘要： A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.
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公开(公告)号：US09012144B2
公开(公告)日：2015-04-21
申请号：US13008182
申请日：2011-01-18
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2527/113
摘要： The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
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