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1.
公开(公告)号:US08512994B2
公开(公告)日:2013-08-20
申请号:US13301204
申请日:2011-11-21
IPC分类号: C12N7/00
CPC分类号: C12N15/86 , A61K48/00 , A61K48/0066 , C12N7/00 , C12N2740/15043 , C12N2740/16042 , C12N2740/16111 , C12N2740/16211 , C12N2740/16311 , C12N2830/60
摘要: The present invention provides nucleic acid, vectors, viruses, and recombinant cells comprising triple-stranded structures, such as those resulting from central initiation and termination of HIV-1 reverse transcription at the center of HIV-1 linear DNA genomes. These triplex structures can act as a cis-determinant of HIV-1 DNA nuclear import, allowing infection of non-dividing target cells. In one aspect, the presence of the DNA triplex sequence in an HIV vector strongly stimulates gene transfer in hematopoietic stem cells. The invention also provides methods of using these triplex structures for making recombinant cells, as well as methods of using the recombinant cells to express proteins of interest both in vitro and in vivo.
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公开(公告)号:US08222029B2
公开(公告)日:2012-07-17
申请号:US12083532
申请日:2006-10-17
申请人: Pierre Charneau , Philippe Despres
发明人: Pierre Charneau , Philippe Despres
CPC分类号: A61K39/12 , A61K2039/5256 , C07K14/005 , C12N2740/15043 , C12N2770/24122 , C12N2770/24134 , C12N2770/24171 , Y02A50/394
摘要: Methods of eliciting humoral responses, methods of immunization, and methods of vaccination using lentiviral vector are disclosed. Additionally, immunogenic compositions and vaccines for West Nile Virus are disclosed.
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公开(公告)号:US08158413B2
公开(公告)日:2012-04-17
申请号:US11250616
申请日:2005-10-17
申请人: Pierre Charneau , Philippe Despres
发明人: Pierre Charneau , Philippe Despres
CPC分类号: C07K14/005 , A61K39/12 , A61K2039/5256 , C12N2740/15043 , C12N2770/24122 , C12N2770/24134 , C12N2770/24171 , Y02A50/394
摘要: Methods of eliciting humoral responses, methods of immunization, and methods of vaccination using lentiviral vector are disclosed. Additionally, immunogenic compositions and vaccines for West Nile Virus are disclosed.
摘要翻译: 公开了引起体液应答的方法,免疫方法和使用慢病毒载体的疫苗接种方法。 另外,公开了用于西尼罗河病毒的免疫原性组合物和疫苗。
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公开(公告)号:US20110086065A9
公开(公告)日:2011-04-14
申请号:US12083532
申请日:2006-10-17
申请人: Pierre Charneau , Philippe Despres
发明人: Pierre Charneau , Philippe Despres
CPC分类号: A61K39/12 , A61K2039/5256 , C07K14/005 , C12N2740/15043 , C12N2770/24122 , C12N2770/24134 , C12N2770/24171 , Y02A50/394
摘要: Methods of eliciting humoral responses, methods of immunization, and methods of vaccination using lentiviral vector are disclosed. Additionally, immunogenic compositions and vaccines for West Nile Virus are disclosed.
摘要翻译: 公开了引起体液应答的方法,免疫方法和使用慢病毒载体的疫苗接种方法。 另外,公开了用于西尼罗河病毒的免疫原性组合物和疫苗。
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5.
公开(公告)号:US20110065089A1
公开(公告)日:2011-03-17
申请号:US12754908
申请日:2010-04-06
申请人: Sylvie Van Der Werf , Nicolas Escriou , Bernadette Crescenzo-Chaigne , Jean-Claude Manuguerra , Frederik Kunst , Benoît Callendret , Jean-Michel Betton , Valérie Lorin , Sylvie Gerbaud , Ana Maria Burguiere , Saliha Azebi , Pierre Charneau , Frédéric Tangy , Chantal Combredet , Jean-François Delagneau , Monique Martin
发明人: Sylvie Van Der Werf , Nicolas Escriou , Bernadette Crescenzo-Chaigne , Jean-Claude Manuguerra , Frederik Kunst , Benoît Callendret , Jean-Michel Betton , Valérie Lorin , Sylvie Gerbaud , Ana Maria Burguiere , Saliha Azebi , Pierre Charneau , Frédéric Tangy , Chantal Combredet , Jean-François Delagneau , Monique Martin
CPC分类号: C07K14/005 , C12N7/00 , C12N2770/20022 , G01N33/569
摘要: The invention relates to a novel strain of severe acute respiratory syndrome (SARS)-associated coronavirus, resulting from a sample collected in Hanoi (Vietnam), reference number 031589, nucleic acid molecules originating from the genome of same, proteins and peptides coded by said nucleic acid molecules and, more specifically, protein N and the applications thereof, for example, as diagnostic reagents and/or as a vaccine.
摘要翻译: 本发明涉及由河内(越南)收集的样品,参考号031589,源自其基因组的核酸分子产生的重症急性呼吸综合征(SARS)相关冠状病毒的新型菌株,由所述 核酸分子,更具体地,蛋白N及其应用,例如作为诊断试剂和/或疫苗。
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6.
公开(公告)号:US20080102057A1
公开(公告)日:2008-05-01
申请号:US11969481
申请日:2008-01-04
CPC分类号: G01N33/5067 , A61K35/12 , C12N5/067 , C12N5/0672 , C12N2501/105 , C12N2501/11 , C12N2501/33 , C12N2503/02 , C12N2510/00
摘要: The present invention relates to a hepatic cell line derived from wild-type mammalian liver tissue by culture methodology, the cells of the cell line being capable of differentiating into hepatocytes and bile duct cells. The present invention also relates to methods of producing such cells as well as to their applications in therapy and as an investigational tool.
摘要翻译: 本发明涉及通过培养方法从野生型哺乳动物肝组织衍生的肝细胞系,细胞系的细胞能够分化成肝细胞和胆管细胞。 本发明还涉及生产这种细胞的方法及其在治疗中的应用和作为研究工具。
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7.
公开(公告)号:US20060040347A1
公开(公告)日:2006-02-23
申请号:US10602663
申请日:2003-06-25
申请人: Pierre Charneau , Veronique Zennou , Huseyin Firat
发明人: Pierre Charneau , Veronique Zennou , Huseyin Firat
IPC分类号: C12N15/867 , C12P21/06
CPC分类号: C12N15/87 , A01K2217/05 , A61K48/00 , A61K2039/51 , C07K14/4748 , C12N7/00 , C12N15/86 , C12N2740/16043 , C12N2740/16062 , C12N2830/50 , C12N2840/20 , C12N2840/203
摘要: The invention concerns a recombinant vector characterised in that it comprises a polynucleotide comprising a central initiation cis-active region (cPPT) and a termination cis-active region (CTS), said regions being of retroviral or retroviral-like origin, said vector further comprising a predetermined nucleotide sequence (transgene or nucleotide sequence of interest) and retranscription regulating, expressing and packaging signals of retroviral or retroviral-like origin.
摘要翻译: 本发明涉及重组载体,其特征在于其包含包含中枢起始顺式活性区(cPPT)和终止顺式 - 活性区(CTS)的多核苷酸,所述区域是逆转录病毒或逆转录病毒样起源的,所述载体还包含 预定的核苷酸序列(感兴趣的转基因或核苷酸序列)和转录调节,表达和包装逆转录病毒或逆转录病毒样起源的信号。
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8.
公开(公告)号:US20050074876A1
公开(公告)日:2005-04-07
申请号:US10677303
申请日:2003-10-03
CPC分类号: G01N33/5067 , A61K35/12 , C12N5/067 , C12N5/0672 , C12N2501/105 , C12N2501/11 , C12N2501/33 , C12N2503/02 , C12N2510/00
摘要: The present invention relates to a hepatic cell line derived from wild-type mammalian liver tissue by culture methodology, the cells of the cell line being capable of differentiating into hepatocytes and bile duct cells. The present invention also relates to methods of producing such cells as well as to their applications in therapy and as an investigational tool.
摘要翻译: 本发明涉及通过培养方法从野生型哺乳动物肝组织衍生的肝细胞系,细胞系的细胞能够分化成肝细胞和胆管细胞。 本发明还涉及生产这种细胞的方法及其在治疗中的应用和作为研究工具。
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公开(公告)号:US09109234B2
公开(公告)日:2015-08-18
申请号:US13695683
申请日:2011-04-29
IPC分类号: C12N15/867 , A61K39/015 , C12N7/00 , C12N15/86 , A61K39/00
CPC分类号: C07K14/445 , A61K39/015 , A61K2039/525 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N15/86 , C12N15/867 , C12N2740/15023 , C12N2740/15043 , C12N2740/16023 , C12N2740/16043 , C12N2810/6081 , Y02A50/412
摘要: The invention relates to lentiviral vector particles pseudotyped with a determined heterologous viral envelope protein or viral envelope proteins originating from a RNA virus and which comprise in its genome at least one recombinant polynucleotide encoding at least one polypeptide(s) carrying epitope(s) of an antigen of a Plasmodium parasite capable of infecting a mammalian host. The lentiviral vector particles are used in order to elicit an immunological response against malaria parasites.
摘要翻译: 本发明涉及用确定的异源病毒包膜蛋白或源自RNA病毒的病毒包膜蛋白假型包装的慢病毒载体颗粒,并且在其基因组中包含至少一个重组多核苷酸,所述重组多核苷酸编码至少一个携带表位的多肽 能够感染哺乳动物宿主的疟原虫寄生虫的抗原。 使用慢病毒载体颗粒以引发针对疟疾寄生虫的免疫应答。
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公开(公告)号:US08709799B2
公开(公告)日:2014-04-29
申请号:US13453784
申请日:2012-04-23
IPC分类号: C12N15/00 , A61K39/205 , A61K39/21
CPC分类号: C07K14/005 , A61K39/12 , A61K2039/505 , A61K2039/525 , A61K2039/5256 , A61K2039/545 , C07K16/10 , C07K16/1054 , C07K16/1081 , C07K2317/21 , C07K2317/34 , C07K2317/76 , C12N15/86 , C12N2740/15022 , C12N2740/15034 , C12N2740/15043 , C12N2740/16222 , C12N2760/20222 , C12N2770/24122 , C12N2770/24134 , C12N2810/6081 , Y02A50/386 , Y02A50/388 , Y02A50/39 , Y02A50/394
摘要: The present invention relates to the design of gene transfer vectors and especially provides lentiviral gene transfer vectors suitable for either a unique administration or for iterative administration in a host, and to their medicinal application (such as vaccination against Immunodeficiency Virus, especially suitable in human hosts). Gene transfer vectors can be either integrative or non-integrative vectors. The invention encompasses prophylactic, therapeutic, symptomatic, and curative treatments of animals, including humans, as well as gene therapy and vaccination in vivo.
摘要翻译: 本发明涉及基因转移载体的设计,特别是提供适用于宿主中独特施用或迭代施用的慢病毒基因转移载体及其药物应用(例如针对免疫缺陷病毒的疫苗接种,特别适用于人宿主 )。 基因转移载体可以是整合或非整合载体。 本发明包括对动物(包括人)的预防,治疗,症状和治疗性治疗,以及基因治疗和体内疫苗接种。
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