公开(公告)号：US08021613B2

公开(公告)日：2011-09-20

申请号：US11900263

申请日：2007-09-11

Applicant: Norman H. Fontaine ,  Prantik Mazumder ,  Mark A. Quesada ,  Eric J. Mozdy ,  Po Ki Yuen

Inventor： Norman H. Fontaine ,  Prantik Mazumder ,  Mark A. Quesada ,  Eric J. Mozdy ,  Po Ki Yuen

IPC: G01N15/06 ,  G01N21/00 ,  G01N21/76

CPC classification number: G01N21/7743 ,  G01N21/05 ,  G01N21/276 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/108 ,  G01N2201/1211 ,  G01N2201/1217 ,  G01N2201/127 ,  G01N2201/12723 ,  G01N2201/128 ,  Y10T436/117497 ,  Y10T436/2575

Abstract: A system and method are described herein for self-referencing a sensor that is used to detect a biomolecular binding event and/or kinetics which occur in a sample solution flowing along side a reference solution in a micron-sized deep flow channel.

Abstract translation: 本文描述了一种系统和方法，用于自我参考用于检测在微米尺寸深流动通道中沿着参考溶液侧流动的样品溶液中发生的生物分子结合事件和/或动力学的传感器。

公开(公告)号：US20080003147A1

公开(公告)日：2008-01-03

申请号：US11824420

申请日：2007-06-29

Applicant: William J. Miller ,  Mark L. Morrell ,  Todd M. Roswech ,  Po Ki Yuen

Inventor： William J. Miller ,  Mark L. Morrell ,  Todd M. Roswech ,  Po Ki Yuen

IPC: B01L3/02

CPC classification number: G01N35/1072 ,  B01L3/5027 ,  G01N35/1074

Abstract: A fluid handling system for transferring fluids is disclosed. The fluid handling system incorporates a plurality of transfer units for dispensing and/or collecting fluids as part of a closed analytical system. The plurality of transfer units are capable of functioning independently and/or collectively when assembled in a unitary array format. Methods of making and using the fluid handling system are also disclosed. The fluid handling system allows a sub-array of transfer units to shift in a vertical direction independent from a position of another sub-array of transfer units that are assembled within the same fluid head. Therefore, the fluid handling system facilitates multi-port fluid delivery and collection in an array format. Further, the fluid handling system aligned with a sensing surface enables label-free detection assays to be performed in an analytical system.

Abstract translation: 公开了一种用于输送流体的流体处理系统。 流体处理系统包括用于分配和/或收集流体作为封闭分析系统的一部分的多个转运单元。 当以单一阵列格式组装时，多个传送单元能够独立地和/或共同地起作用。 还公开了制造和使用流体处理系统的方法。 流体处理系统允许传送单元的子阵列在垂直方向上移动，独立于组装在同一流体头内的另一传送单元子阵列的位置。 因此，流体处理系统便于以端口流体输送和以阵列格式收集。 此外，与感测表面对准的流体处理系统使得能够在分析系统中进行无标记检测分析。

公开(公告)号：US20140057310A1

公开(公告)日：2014-02-27

申请号：US13675396

申请日：2012-11-13

Applicant: Ye Fang ,  Ann MeeJin Ferrie ,  Norman Henry Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Norman Henry Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

IPC: G01N33/50

CPC classification number: G01N33/5008 ,  G01N33/54373 ,  G01N33/554

Abstract: Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed.

Abstract translation: 公开了使用无标签光学生物传感器进行细胞测定的组合物和方法。 在某些实施方案中，测定可以以高通量方法进行并且可以被复用。

公开(公告)号：US08338116B2

公开(公告)日：2012-12-25

申请号：US13281996

申请日：2011-10-26

Applicant: Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

Inventor： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

IPC: G01N33/567 ,  G01N21/76 ,  C12Q1/02

CPC classification number: G01N33/5008 ,  G01N33/54373 ,  G01N33/554

Abstract: Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in highthough put methods and can be multiplexed.

Abstract translation: 公开了使用无标签光学生物传感器进行细胞测定的组合物和方法。 在某些实施方案中，所述测定可以以放置方式进行并且可以被复用。

公开(公告)号：US20120070878A1

公开(公告)日：2012-03-22

申请号：US13234490

申请日：2011-09-16

Applicant: Katherine A. Fink ,  Vasiliy Nikolaevich Goral ,  Hui Su ,  Po Ki Yuen

Inventor： Katherine A. Fink ,  Vasiliy Nikolaevich Goral ,  Hui Su ,  Po Ki Yuen

IPC: C12N1/00 ,  C08J9/26 ,  B01J19/00 ,  C12M1/00 ,  C01B31/00

CPC classification number: B01L3/502723 ,  B01L3/502707 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/0816 ,  B01L2300/10 ,  B01L2400/0406 ,  C12M23/16

Abstract: A micro fluidic apparatus includes (i) a first conduit; (ii) a second conduit; and (iii) a first interconnected microporous network in communication with the first and second conduits and configured to allow diffusion of gas between the first and second conduits. The microporous network comprises poly(dimethylsiloxane) (PDMS) and prevents flow of aqueous fluid between the first and second conduits through the microporous network.

Abstract translation: 微流体装置包括（i）第一导管; （ii）第二导管; 和（iii）与第一和第二导管连通的第一互连的微孔网络，并且被配置为允许气体在第一和第二导管之间扩散。 微孔网络包括聚（二甲基硅氧烷）（PDMS）并且防止第一和第二导管之间的水性流体通过微孔网络流动。

公开(公告)号：US20120052565A1

公开(公告)日：2012-03-01

申请号：US13218103

申请日：2011-08-25

Applicant: Michael Edward DeRosa ,  Joydeep Lahiri ,  Hongwei Hanna Rao ,  Po Ki Yuen

Inventor： Michael Edward DeRosa ,  Joydeep Lahiri ,  Hongwei Hanna Rao ,  Po Ki Yuen

IPC: C12M3/00 ,  C23F1/00

CPC classification number: C08J7/02 ,  C08J2323/08 ,  C08J2325/06 ,  C12M23/22 ,  C12M25/14

Abstract: A cell culture article has substrate that is predominantly opaque and that provides a three-dimensional (e.g., irregular) surface, but incorporates an optically transparent, substantially regular (e.g., two-dimensional) surface to serve as a microscopic observation and imaging window for the 3D cell culture. In many embodiments, the 3D portion of the substrate occupies greater than 99% of the surface while the 2D portion occupies less than 1% of the surface so as not to substantially disrupt the general 3D culture environment.

Abstract translation: 细胞培养物具有主要是不透明的并且提供三维（例如，不规则）表面的基底，但是包含光学透明的，基本上规则的（例如，二维）表面，用作微观观察和成像窗口 3D细胞培养。 在许多实施例中，基板的3D部分占据表面的大于99％，而2D部分占据表面的1％以下，以免基本上破坏一般的3D文化环境。

公开(公告)号：US20120052250A1

公开(公告)日：2012-03-01

申请号：US13217912

申请日：2011-08-25

Applicant: Michael Edward DeRosa ,  Po Ki Yuen

Inventor： Michael Edward DeRosa ,  Po Ki Yuen

IPC: B32B3/30 ,  B44C1/22

CPC classification number: C08J7/02 ,  B01L3/502707 ,  B01L2300/0887 ,  C08J2325/06 ,  Y10T428/24612

Abstract: Polymeric sheets having interconnected microporous networks are generated by contacting the sheets with a composition including solvent and non-solvent in an appropriate ratio and removing the composition from the sheet. Such sheets may be advantageously used in micro fluidic devices for a variety of purposes.

Abstract translation: 通过使片材与包含溶剂和非溶剂的组合物以适当的比例接触而产生具有相互连接的微孔网络的聚合物片材，并从片材中除去组合物。 为了各种目的，这种片可有利地用于微流体装置中。

公开(公告)号：US20110305598A1

公开(公告)日：2011-12-15

申请号：US13213744

申请日：2011-08-19

Applicant: Norman H. Fontaine ,  Prantik Mazumder ,  Mark A. Quesada ,  Eric J. Mozdy ,  Po Ki Yuen

Inventor： Norman H. Fontaine ,  Prantik Mazumder ,  Mark A. Quesada ,  Eric J. Mozdy ,  Po Ki Yuen

IPC: G01N33/48

CPC classification number: G01N21/7743 ,  G01N21/05 ,  G01N21/276 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/108 ,  G01N2201/1211 ,  G01N2201/1217 ,  G01N2201/127 ,  G01N2201/12723 ,  G01N2201/128 ,  Y10T436/117497 ,  Y10T436/2575

Abstract: A microfluidic device is described herein which comprises a micron-sized deep flow channel and a sensor. The micron-sized deep flow channel is configured such that a sample solution and a reference solution flow side-by-side to one another in a single sensing region of the sensor. The single sensing region is divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with the sample solution and the reference solution that flow side-by-side to one another in a longitudinal direction within the micron-sized deep flow channel.

Abstract translation: 本文描述了一种微流体装置，其包括微米级的深流动通道和传感器。 微米尺寸深流通道被配置为使得样品溶液和参比溶液在传感器的单个感测区域中彼此并排流动。 单个感测区域被分成彼此邻接的检测区域和参考区域，它们分别与样品溶液和在微米内沿纵向彼此并排流动的参比溶液接合。 深流道。

公开(公告)号：US20090186776A1

公开(公告)日：2009-07-23

申请号：US12137918

申请日：2008-06-12

Applicant: Brian L. Webb ,  Jinlin Peng ,  Michael D. Brady ,  Mircea Despa ,  Keith A. Horn ,  Joydeep Lahiri ,  David M. Root ,  James B. Stamatoff ,  Po Ki Yuen

Inventor： Brian L. Webb ,  Jinlin Peng ,  Michael D. Brady ,  Mircea Despa ,  Keith A. Horn ,  Joydeep Lahiri ,  David M. Root ,  James B. Stamatoff ,  Po Ki Yuen

IPC: C40B30/10 ,  C40B60/12

CPC classification number: B01L3/50853 ,  B01J2219/00317 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00662 ,  B01J2219/00707 ,  B01L3/5025 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0636 ,  B01L2300/0829 ,  C12Q1/6837 ,  C40B60/14 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/54386 ,  G01N35/028

Abstract: A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.

Abstract translation: 提供了用于进行生物或化学分析的装置和方法。 该装置包括从支撑板突出的三维微柱阵列。 每个微柱具有远离支撑板的相对平面的第一表面。 多个不同生物材料的阵列可以附接到第一表面。 该装置与现有的微量滴定板结合使用可以提高使用微阵列进行结合测定的高通量能力。

公开(公告)号：US20080160602A1

公开(公告)日：2008-07-03

申请号：US12002656

申请日：2007-12-18

Applicant: Lin He ,  Jinlin Peng ,  Youchun Shi ,  Brian L. Webb ,  Po Ki Yuen

Inventor： Lin He ,  Jinlin Peng ,  Youchun Shi ,  Brian L. Webb ,  Po Ki Yuen

IPC: C12M1/34

CPC classification number: B01L3/5025 ,  B01L3/50853 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0636 ,  B01L2300/0829 ,  C12Q1/6837 ,  G01N33/54366 ,  G01N35/028 ,  Y10T436/2575

Abstract: A biological assay device for use in molecular biology, pharmaceutical research, genomic analysis, combinatorial chemistry, and in the general field of the analysis of molecules that may be deposited on supports of various kinds is provided. Specifically, the present invention includes a fluidic or microfluidic device, which integrates fluidic capability into existing multi-well plates of standard configuration, for performing either single or continuous fluidic manipulations in a high-throughout format. Methods for the use and manufacture of these devices are also provided.

Abstract translation: 提供了用于分子生物学，药物研究，基因组分析，组合化学以及可能沉积在各种载体上的分子分析的一般领域的生物测定装置。 具体地，本发明包括流体或微流体装置，其将流体性能集成到现有的标准配置的多孔板中，用于以高整体格式进行单一或连续的流体操纵。 还提供了这些装置的使用和制造方法。