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公开(公告)号：US07326826B2
公开(公告)日：2008-02-05
申请号：US11107959
申请日：2005-04-15
摘要： The invention features a method of selecting for a grape somatic embryo or grape plant having resistance to a plant pathogen using a liquid culture medium. The method including the steps of (a) culturing a grape embryonic culture in a first liquid culture medium to grow a cellular suspension culture, the first liquid culture medium including a plant growth regulator and a filtrate from a plant pathogen culture; (b) recovering an embryogenic cell or embryogenic cell mass from the cellular suspension culture; and (c) culturing the embryogenic cell or embryogenic cell mass in a second liquid culture and in a third culture media to select a grape somatic embryo or a grape plant having resistance to a plant pathogen.
摘要翻译： 本发明的特征在于使用液体培养基选择对植物病原体具有抗性的葡萄体细胞胚或葡萄植物的方法。 该方法包括以下步骤：（a）在第一液体培养基中培养葡萄胚胎培养物以生长细胞悬浮培养物，第一液体培养基包括植物生长调节剂和来自植物病原体培养物的滤液; （b）从细胞悬浮培养物回收胚胎发生细胞或胚胎发生细胞团; 和（c）在第二液体培养物和第三培养基中培养胚胎发生细胞或胚胎发生细胞团，以选择葡萄体细胞胚或对植物病原体具有抗性的葡萄植物。
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公开(公告)号：US06455312B1
公开(公告)日：2002-09-24
申请号：US09311823
申请日：1999-05-13
IPC分类号： C12N500
摘要： The invention features methods of producing a plant or a mature somatic embryo from an embryogenic cell or embryogenic culture. The method includes the steps of: (a) providing a liquid culture that includes an embryogenic cell; (b) recovering embryogenic cell from the culture; (c) transferring the embryogenic cell to a second culture; and (d) growing a mature somatic grape embryo from the embryogenic cell.
摘要翻译： 本发明的特征在于从胚胎发生细胞或胚发生培养物中产生植物或成熟体细胞胚的方法。 该方法包括以下步骤：（a）提供包含胚胎发生细胞的液体培养物; （b）从培养物中回收胚胎发生细胞; （c）将胚胎发生细胞转移到第二培养物; 和（d）从胚胎发生细胞生长成熟的体细胞葡萄胚胎。
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