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公开(公告)号：US06818436B2
公开(公告)日：2004-11-16
申请号：US10235971
申请日：2002-09-05
IPC分类号： C12M300
CPC分类号： H01L23/3677 , H01L2924/0002 , H01L2924/00
摘要： A micro array chip is formed by high-speed in jection molding. A chip body has a thickness of 1 mm and each well has a capacity of 1.2 &mgr;L with the bottom having a wall thickness of 250 &mgr;m. An opening of each well is surrounded by an annular protrusion part, which is raised from a surface of the chip body by a height of 200 &mgr;m. The openings of wells may be closed with a sheet of sealant made of aluminum or a resin. An overall shape of the micro array chip is rectangular and is a flat plate with a level bottom surface. Therefore, the micro array chip can be used with a heat block in flat plate form, which is independent of chip specifications such as a number of wells and their shape.
摘要翻译： 微阵列芯片通过高速成型而形成。 芯片的厚度为1mm，每个孔的容积为1.2μm，底部的壁厚为250μm。 每个孔的开口由环形突出部分围绕，该突出部分从芯片体的表面升高200μm的高度。 孔的开口可以用由铝或树脂制成的密封剂片封闭。 微阵列芯片的整体形状是矩形的，并且是具有水平底面的平板。 因此，微阵列芯片可以与平板形式的热块一起使用，其独立于芯片规格，例如多个孔及其形状。
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2.发明授权Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment 失效肽C-末端片段序列分析方法及收集肽片段的方法
公开(公告)号：US5470703A
公开(公告)日：1995-11-28
申请号：US136761
申请日：1993-10-15
申请人： Kiyoshi Nokihara , Rintaro Yamamoto
发明人： Kiyoshi Nokihara , Rintaro Yamamoto
CPC分类号： C12Q1/37 , C07K1/128 , G01N33/6821 , Y10S436/81 , Y10T436/255
摘要： The present invention relates to a method for peptide C-terminal fragment sequence analysis, in which the fragment collection is carried out on an allylamine group-derivatized polymer membrane or on allylamine group-derivatized glass fiber filter paper; the collected C-terminal fragment is immobilized thereon using a water-soluble carbodiimide etc.; and the obtained immobilized product is subjected directly to amino acid sequence analysis. The present invention also relates to an apparatus for collecting a peptide fragment. According to the method of the present invention, peptides which are rich in hydrophobic groups in their C-terminus and are therefore difficult to trap with polyvalent ion carriers, currently used in the gas-phase sequencer, can be completely analyzed up to their C-terminus. Also, amino acid sequence analysis can be made even when the amount of C-terminal fragments is very small. In addition, since collection and immobilization of fragments can be done in the same bottle, the risks of contamination and mechanical loss are very low.
摘要翻译： 本发明涉及一种肽C-末端片段序列分析方法，其中片段收集是在烯丙基胺基衍生的聚合物膜上或在烯丙胺基衍生的玻璃纤维滤纸上进行的; 收集的C-末端片段使用水溶性碳二亚胺等固定在其上; 将获得的固定化产物直接进行氨基酸序列分析。 本发明还涉及一种收集肽片段的装置。 根据本发明的方法，可以完全分析其C-末端富含疏水性基团并且因此难以与当前用于气相测序仪中的多价离子载体捕获的肽， 终点。 此外，即使C末端片段的量非常小，也可以进行氨基酸序列分析。 此外，由于碎片的收集和固定可以在同一瓶中进行，污染和机械损失的风险非常低。
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公开(公告)号：US5431882A
公开(公告)日：1995-07-11
申请号：US154515
申请日：1993-11-19
CPC分类号： G01N33/6803 , C07K1/12
摘要： The present invention relates to an apparatus for collecting a peptide fragment, wherein a C-terminal peptide fragment is collected from a peptide fragment mixture resulting from specific cleavage of the peptide bond between a lysine residue and the C-terminal amino acid residue adjacent thereto. The apparatus has an immobilizing means, a cleaving means, a recovering means and a control means. According to the present invention, since the control means sequentially executes the immobilizing means, cleaving means and recovering means, no skillful work is required in peptide fragment collection, making it possible to collect the carboxyl-terminal peptide fragment with simple operation and high reproducibility.
摘要翻译： 本发明涉及一种用于收集肽片段的装置，其中从由赖氨酸残基和与其相邻的C末端氨基酸残基之间的肽键的特异性切割得到的肽片段混合物收集C末端肽片段。 该装置具有固定装置，切割装置，恢复装置和控制装置。 根据本发明，由于控制装置依次执行固定装置，切割装置和恢复装置，因此在肽片段收集中不需要熟练的工作，使得可以以简单的操作和高再现性收集羧基末端肽片段。
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公开(公告)号：US5395594A
公开(公告)日：1995-03-07
申请号：US933729
申请日：1992-08-24
IPC分类号： B01J19/00 , B01J19/28 , C07K1/04 , C07K5/08 , C07K7/06 , C07K14/575 , C07K14/655 , C07K14/70 , C40B40/10 , C40B60/14 , B01J8/02
CPC分类号： B01J19/004 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/00308 , B01J2219/00373 , B01J2219/00423 , B01J2219/00493 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： The present invention is directed to a simultaneous multiple chemical synthesizer comprising a number of reaction vessels wherein each vessel has a filter in the bottom portion thereof, a number of needles wherein each needle is connected to an aspiration injection line of a reaction mixture and a gas supply line in connection with each reaction vessel and each needle does not touch the resin contained in the reaction vessel, a number of arms which are horizontally and vertically movable and hold the respective needles, a bubbling gas line and a waste discharge line in connection with each reaction vessel, wherein each line is connected to the bottom portion of each reaction vessel, a number of purging means which move synchronously with said waste discharge lines, and means for washing the portions of the needles and purge means which contact with the reaction reagents.
摘要翻译： 本发明涉及一种同时多重化学合成器，其包括多个反应容器，其中每个容器在其底部具有过滤器，多个针，其中每个针连接到反应混合物的抽吸注入管线和气体 与每个反应容器相关的供应管线，并且每个针不接触包含在反应容器中的树脂，可水平和垂直移动并保持各针的多个臂，鼓泡气管线和排放废水排放管线与 每个反应容器，其中每条管线连接到每个反应容器的底部，与所述废物排放管线同步移动的多个清洗装置，以及用于清洗针头的部分和与反应试剂接触的清洗装置的装置 。
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公开(公告)号：US20060191793A1
公开(公告)日：2006-08-31
申请号：US11360676
申请日：2006-02-24
申请人： Rintaro Yamamoto
发明人： Rintaro Yamamoto
IPC分类号： G01N27/447
CPC分类号： G01N27/447
摘要： An electrophoresis plate has a plurality of separation channels provided inside a substrate. The separation channels are arranged along the surface of the substrate without intersecting each other, and the separation channels have openings on both ends on the substrate surface. When a voltage is applied between the two ends of the channel, a sample is separated by electrophoresis along the channel. The openings on one end are placed in a matrix pattern as sample inlet ports, and the pitch of that placement is set equal to the pitch of the wells of a sample plate containing the samples to be injected into the sample inlet ports. Since the pitch of the sample inlet ports pattern is equal to the pitch of the sample plate wells, sample injection can be performed quickly and conveniently even with a large number of separation channels.
摘要翻译： 电泳板具有设置在基板内的多个分离通道。 分离通道沿着基板的表面布置而不相交，并且分离通道在基板表面的两端具有开口。 当在通道的两端之间施加电压时，通过沿通道的电泳分离样品。 将一端的开口放置在作为样品入口的矩阵图案中，并且将该放置的间距设置为等于包含待注入样品入口的样品的样品板的孔的间距。 由于样品入口图案的间距等于样品板孔的间距，即使使用大量分离通道也能快速方便地进行样品注入。
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公开(公告)号：US06428670B1
公开(公告)日：2002-08-06
申请号：US09443720
申请日：1999-11-19
IPC分类号： C25B1508
CPC分类号： G01N27/44704 , G01N27/44782
摘要： A holder for a capillary cassette closes a chamber, and is fixed to a detection side holder fixing member. An acidic solution container, an alkaline solution container, a pure water container and a drain container are arranged on a reservoir stage having a dry chamber. A holder up/down mechanism and a stage moving mechanism successively bring an end of a capillary array into contact with an acidic solution, an alkaline solution, pure water and nitrogen gas, and the chamber is decompressed for successively introducing these into capillary columns and performing pretreatment. Thereafter a gel container is arranged in the chamber, which in turn is pressurized for charging the capillary columns with a gel.
摘要翻译： 用于毛细管盒的保持器封闭腔室，并固定到检测侧保持器固定构件。 酸性溶液容器，碱性溶液容器，纯水容器和排水容器布置在具有干燥室的储存台上。 支架上下机构和台架移动机构依次使毛细管阵列的一端与酸性溶液，碱性溶液，纯水和氮气接触，并且将腔室减压以将它们连续地引入毛细管柱中并进行 预处理。 此后，将凝胶容器设置在腔室中，该容器又被加压以用凝胶装载毛细管柱。
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公开(公告)号：US07531076B2
公开(公告)日：2009-05-12
申请号：US11326368
申请日：2006-01-06
IPC分类号： G01N27/453
CPC分类号： G01N27/44782 , Y10S83/95 , Y10T29/5148 , Y10T29/53391 , Y10T83/0596
摘要： Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. it fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.
摘要翻译： 毛细管柱（102）穿过并被插入橡胶板（14）中，橡胶板通过橡胶的弹力保持和固定，并且二维地布置在样品注射侧。 它通过将保持板（6a）从下方夹持而在安装在平面上的毛细管柱（102）上固定，并在检测侧从橡胶板（16）上方固定。 为了将毛细管柱（102）压靠在保持板6a上并将其固定在橡胶板（16）上，将橡胶板（16）固定在两侧的保持板（6a）上的保持板（6b） 提供毛细管柱（102）的布置。
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公开(公告)号：US20080087547A1
公开(公告)日：2008-04-17
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/447 , G01N21/64
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译： 为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有 连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液 连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。
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公开(公告)号：US5356596A
公开(公告)日：1994-10-18
申请号：US22036
申请日：1993-02-24
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00288 , B01J2219/00308 , B01J2219/00454 , B01J2219/005 , B01J2219/00725 , C40B40/10 , C40B50/14 , C40B60/14
摘要： In connection with an automated peptide synthesizer, an apparatus and associated method through which peptides produced in simultaneous multiple synthesis are together isolable. Cleavage to liberate the peptides from linkage to the support matrix of the solid phase is carried out in the same vessels in which the synthesizing reactions took place. Prior to cleavage, a drainage port of each reaction vessel is closed off by a cap. The reaction vessel is inserted into a centrifuge tube, which in turn is put into a rack supporting a number of tubes equal to the number of channels of the peptide synthesizing apparatus. After the peptides are cleaved and dissolved into cleaving solution added into each of the reaction vessels, the caps are removed from the drainage ports of each reaction vessel, and the vessel is returned into the centrifuge tube. A plastic jet-fitting attached to the nozzle tip of a blow unit pressure gun is inserted into and pressed into contact against the supply opening of the reaction vessel. Operating a trigger of the pressure gun releases pressurized inert gas into the reaction chamber. The peptide-dissolved cleaving solution is thereby passed through the drainage port, and is thus transferred in liquid phase into the centrifuge tube as filtrate. Associated with the blow unit pressure gun, a needle tube fitting is provided for localized desiccation of wetted peptide following precipitation and centrifuging.
摘要翻译： 关于自动肽合成仪，同时多重合成中产生的肽一起可分离的装置和相关方法。 在与发生合成反应的相同的容器中进行从固定相的支持基质的连接释放肽的切割。 在切割之前，每个反应容器的排水口由盖封闭。 将反应容器插入到离心管中，该离心管又被放入支架中，该支架中的多个管等于肽合成装置的通道数。 在肽被切割并溶解到每个反应容器中的裂解溶液中之后，从每个反应容器的排出口移除盖子，并将容器返回到离心管中。 将附接到吹塑单元压力枪的喷嘴尖端的塑料喷射管插入并压接到反应容器的供应开口。 操作压力枪的触发器将加压的惰性气体释放到反应室中。 因此，肽溶解的解离溶液通过排出口，因此以液相方式转移到作为滤液的离心管中。 与吹气单元压力枪相关联，提供针管配件，用于沉淀和离心后的湿肽的局部干燥。
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公开(公告)号：US07655125B2
公开(公告)日：2010-02-02
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/453
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译： 为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有 连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液 连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。
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