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    Apparatus for providing an environment of controlled water activity for storage of nematodes
    1.
    发明授权
    Apparatus for providing an environment of controlled water activity for storage of nematodes 失效
    用于提供用于储存线虫的受控水活性环境的装置

    公开(公告)号：US06841380B2

    公开(公告)日：2005-01-11

    申请号：US10146073

    申请日：2002-05-16

    申请人： Robin Anthony Bedding ,  Simone Daniela Clark ,  Karen Louise Butler

    发明人： Robin Anthony Bedding ,  Simone Daniela Clark ,  Karen Louise Butler

    IPC分类号： A01N1/02 ,  A01K67/00 ,  A01K67/033 ,  A01N25/34 ,  A01N63/00 ,  C12N5/18 ,  C12M1/00 ,  C12M3/00 ,  C12N5/00 ,  C12N15/74

    CPC分类号： A01K67/033 ,  A01N63/00 ,  A01N25/34 ,  A01N2300/00

    摘要： Third stage juvenile (J3) entomopathogenic nematodes are prepared for storage by being induced into a state of cryptobiosis. The induction of cryptobiosis is effected by mixing an aqueous cream of the J3 nematodes with anhydrous, small particles of non-fibrous cellulose. The cryptobiotic J3 nematodes are stored in a container, fitted with an attachment which maintains the water activity in the container at a required value. The attachment includes a rigid tube that connects the interior of the container with a chamber that is vented to ambient atmosphere by small apparatus. The chamber contains water-absorbent material saturated with water or with a saturated salt solution, and the tube contains an air-permeable plug. An alternative attachment comprises a plastic envelope as the chamber, one face of which is stuck to the wall of the container. Small aperatures in the face are aligned with apertures in the container wall. Small apertures in the other face connect the inside of the envelope to ambient atmosphere.

    摘要翻译： 制备第三阶段幼虫（J3）昆虫病原线虫，通过诱导进入隐孢子虫病状进行储存。 通过将J3线虫的水性乳膏与无水的非纤维状纤维素的小颗粒混合来实现致密生物的诱导。 密生生物J3线虫储存在装有附件的容器中，该附件将容器中的水分活性保持在所需值。 附件包括刚性管，其将容器的内部与通过小型设备排放到周围环境的室相连。 该室含有用水或饱和盐溶液饱和的吸水材料，并且该管含有透气性塞子。 替代附件包括作为腔室的塑料封套，其一面粘在容器的壁上。 表面上的小孔与容器壁中的孔对齐。 另一面的小孔将信封的内部连接到环境大气。

    Method for preparing entomopathogenic nematodes for storage by mixing with non-fibrous cellulose particles of less than 300 &mgr;M
    2.
    发明授权
    Method for preparing entomopathogenic nematodes for storage by mixing with non-fibrous cellulose particles of less than 300 &mgr;M 失效
    用于通过与小于300μm的非纤维状纤维素颗粒混合来制备昆虫病原线虫的方法

    公开(公告)号：US06407310B1

    公开(公告)日：2002-06-18

    申请号：US09806143

    申请日：2001-03-28

    申请人： Robin Anthony Bedding ,  Simone Daniela Clark ,  Michael James Lacey ,  Karen Louise Butler

    发明人： Robin Anthony Bedding ,  Simone Daniela Clark ,  Michael James Lacey ,  Karen Louise Butler

    IPC分类号： A01K6700

    CPC分类号： A01K67/033 ,  A01N63/00 ,  A01N25/34 ,  A01N2300/00

    摘要： Third stage juvenile (J3) entomopathogenic nematodes are prepared for storage by being induced into a state of cryptobiosis. The induction of cryptobiosis is effected by mixing an aqueous cream of the J3 nematodes with anhydrous, small particles (average maximum dimension less than 300 &mgr;m) of non-fibrous cellulose. The proportions of the aqueous cream and non-fibrous cellulose particles are such that, after equilibration, the mixture has a water activity in the range 0.80 to 0.995. Preferably an anti-fungal agent is included in the aqueous cream. To store the cryptobiotic J3 nematodes, the mixture is preferably kept in a container, fitted with an attachment which maintains the water activity in the container at a required value. The attachment includes a rigid tube that connects the interior of the container with a chamber that is vented to ambient atmosphere by small apparatus. When in use the chamber contains water-absorbent material saturated with water or with a saturated salt solution, and the tube contains an air-permeable plug. An alternative attachment comprises a plastic envelope, one face of which is stuck to the wall of the container. Small apertures in the face are aligned with apertures in the container wall. Small apertures in the other face connect the inside of the envelope to ambient atmosphere. In use, the envelope contains a water-absorbent material saturated with water or a salt solution, and at least one spacer member.

    摘要翻译： 制备第三阶段幼虫（J3）昆虫病原线虫，通过诱导进入隐孢子虫病状进行储存。 通过将J3线虫的水性乳膏与非纤维状纤维素的无水，小颗粒（平均最大尺寸小于300μm）混合来实现致密生物的诱导。 水性乳液和非纤维状纤维素颗粒的比例使得在平衡后，该混合物的水分活度在0.80-0.995的范围内。 优选地，水性乳膏中包含抗真菌剂。 为了储存密生生物J3线虫，优选将混合物保存在容器中，该容器装有将容器中的水活性保持在所需值的附件。 附件包括刚性管，其将容器的内部与通过小型设备排放到周围环境的室相连。 当使用时，该室含有被水或饱和盐溶液饱和的吸水材料，并且该管含有透气性的塞子。 替代附件包括塑料封套，其一面贴在容器的壁上。 面中的小孔与容器壁中的孔对齐。 另一面的小孔将信封的内部连接到环境大气。 在使用中，信封包含饱和水或盐溶液的吸水材料和至少一个间隔件。