公开(公告)号：US20120040464A1

公开(公告)日：2012-02-16

申请号：US12965195

申请日：2010-12-10

申请人： Feng Ling ,  Takehiko Shibata ,  Rong Niu ,  Minoru Yoshida ,  Yu-ichi Goto

发明人： Feng Ling ,  Takehiko Shibata ,  Rong Niu ,  Minoru Yoshida ,  Yu-ichi Goto

IPC分类号： C12N15/01 ,  C12N5/071

CPC分类号： C12N5/04 ,  A01H1/06

摘要： It is an object of the present invention to provide a method, which comprises allowing a eukaryotic cell to come into contact with a reactive oxygen species or a chemical species that generates such a reactive oxygen species in the cell, and thereby changing the percentage of mutant mtDNA (mitochondrial genomic DNA) in the cell, and cells obtained by the above-described method.The present invention relates to a method, which comprises allowing cells to come into contact with a reactive oxygen species by, for example, adding the reactive oxygen species such as hydrogen peroxide to a medium containing the cells, and then culturing the cells under suitable culture conditions, so that the percentage of mtDNA having specific mutation in the cell can be changed. In addition, the present invention also relates to cells, in which the percentage of the mtDNA having specific mutation has been changed by the above-described method.

摘要翻译： 本发明的目的是提供一种方法，其包括使真核细胞与活性氧物质或在细胞中产生这种活性氧的化学物质接触，从而改变突变体 细胞中的mtDNA（线粒体基因组DNA），以及通过上述方法获得的细胞。 本发明涉及一种方法，其包括使细胞与活性氧物质接触，例如通过将诸如过氧化氢的活性氧物质加入到含有细胞的培养基中，然后在合适的培养基下培养细胞 条件，使得可以改变在细胞中具有特异性突变的mtDNA的百分比。 此外，本发明还涉及通过上述方法改变具有特异性突变的mtDNA的百分比的细胞。

公开(公告)号：US08691578B2

公开(公告)日：2014-04-08

申请号：US12965195

申请日：2010-12-10

申请人： Feng Ling ,  Takehiko Shibata ,  Rong Niu ,  Minoru Yoshida ,  Yu-ichi Goto

发明人： Feng Ling ,  Takehiko Shibata ,  Rong Niu ,  Minoru Yoshida ,  Yu-ichi Goto

IPC分类号： C12N15/01 ,  C07H21/04

CPC分类号： C12N5/04 ,  A01H1/06

摘要： A method includes providing a eukaryotic cell including mutant mtDNA in which there is at least one mutation in the mtDNA, allowing the cell to come into contact with an active oxygen species or a chemical species that generates such an active oxygen species in the cell (e.g., hydrogen peroxide) and thereby changing the percentage of mutant mtDNA (mitochondrial genomic DNA) in the cell as a result of the contact. Also featured are cells obtained by the above-described method.

摘要翻译： 一种方法包括提供包括突变mtDNA的真核细胞，其中mtDNA中存在至少一个突变，允许细胞与活性氧物质或在细胞中产生这种活性氧物质的化学物质接触（例如， ，过氧化氢），从而改变作为接触的结果的细胞中突变体mtDNA（线粒体基因组DNA）的百分比。 还特征在于通过上述方法获得的细胞。