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公开(公告)号:US4704274A
公开(公告)日:1987-11-03
申请号:US666172
申请日:1984-10-26
申请人: Shin Sakuma , Kuniaki Sakamoto , Hisashi Kitagawa , Mitsuo Sakoh , Saneo Nonaka
发明人: Shin Sakuma , Kuniaki Sakamoto , Hisashi Kitagawa , Mitsuo Sakoh , Saneo Nonaka
IPC分类号: A61K39/095 , A61K39/00 , A61K39/10 , A61P31/04 , C07K14/235 , C12P1/04 , C12R1/01 , A61K39/02 , C07K3/20
CPC分类号: C07K14/235 , A61K39/00 , Y10S435/822
摘要: A method for producing highly pure LPF-HA (Leucocytosis-promoting Factor agglutinin) from culture media of Bordetella pertussis in a high yield by an affinity chromatography using a denatured ceruloplasmin as a ligand. The LPF-HA obtained by this invention is highly pure and does not contain almost endotoxin of B. pertussis and is useful for the preparation of various reagents utilizing the physiological activities, a medicine for treating diabetes, and a pertussis vaccine.
摘要翻译: 通过使用变性的血浆铜蓝蛋白作为配体的亲和层析,以高产率从培养的百日咳杆菌培养基生产高纯度LPF-HA(白细胞增多因子血凝素)的方法。 通过本发明获得的LPF-HA是高纯度的,不含有百日咳杆菌的几乎内毒素,并且可用于制备利用生理活性的各种试剂,用于治疗糖尿病的药物和百日咳疫苗。