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    Calcium-binding photoprotein
    1.
    发明授权
    Calcium-binding photoprotein 有权
    钙结合光蛋白

    公开(公告)号：US08772452B2

    公开(公告)日：2014-07-08

    申请号：US12662886

    申请日：2010-05-10

    Applicant: Satoshi Inouye ,  Yuiko Sahara

    Inventor： Satoshi Inouye ,  Yuiko Sahara

    IPC: C07K14/435

    CPC classification number: G01N33/20 ,  C07K14/43595

    Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.

    Abstract translation: 本发明提供了可以以较高灵敏度检测光发射的结合钙的光蛋白。 包含SEQ ID NO：2的氨基酸序列的本发明的蛋白质可用于检测和测量钙离子。 本发明的蛋白质可用作报道蛋白，发光标记等。本发明的多核苷酸可用作报道基因等。

    Fusion protein having luminescence activity
    2.
    发明授权
    Fusion protein having luminescence activity 有权
    具有发光活性的融合蛋白

    公开(公告)号：US08481702B2

    公开(公告)日：2013-07-09

    申请号：US13763212

    申请日：2013-02-08

    Applicant: Satoshi Inouye ,  Yuiko Sahara ,  Junichi Sato

    Inventor： Satoshi Inouye ,  Yuiko Sahara ,  Junichi Sato

    IPC: C07H21/02 ,  C12N15/00 ,  C12N1/20

    CPC classification number: G01N33/581 ,  C07K14/43509 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/60 ,  C12N9/0069 ,  C12Q1/66

    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.

    Abstract translation: 该融合蛋白包含（1）包含SEQ ID NO：18的氨基酸序列的第一区域和（2）第二区域，其包含含有至少一个半胱氨酸残基的多肽的氨基酸序列，用于通过 硫醇基可以通过化学改性进行改性，因此具有高的发光活性的催化能力，并且高度可用于一般目的。

    FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY
    3.
    发明申请
    FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY 有权
    具有发光活性的融合蛋白

    公开(公告)号：US20130143233A1

    公开(公告)日：2013-06-06

    申请号：US13763212

    申请日：2013-02-08

    Applicant: Satoshi INOUYE ,  Yuiko SAHARA ,  Junichi SATO

    Inventor： Satoshi INOUYE ,  Yuiko SAHARA ,  Junichi SATO

    IPC: C12N9/00 ,  C12Q1/66 ,  G01N33/58

    CPC classification number: G01N33/581 ,  C07K14/43509 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/60 ,  C12N9/0069 ,  C12Q1/66

    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.

    Abstract translation: 该融合蛋白包含（1）包含SEQ ID NO：18的氨基酸序列的第一区域和（2）第二区域，其包含含有至少一个半胱氨酸残基的多肽的氨基酸序列，用于通过 硫醇基可以通过化学改性进行改性，因此具有高的发光活性的催化能力，并且高度可用于一般目的。

    Recombinant photoproteins and their conjugates
    9.
    发明授权
    Recombinant photoproteins and their conjugates 有权
    重组光蛋白及其缀合物

    公开(公告)号：US07241864B2

    公开(公告)日：2007-07-10

    申请号：US10400630

    申请日：2003-03-28

    Applicant: Satoshi Inouye

    Inventor： Satoshi Inouye

    IPC: G01N33/53 ,  C07K14/435 ,  C12N15/12 ,  C12N15/63 ,  C12N5/10

    CPC classification number: C07K14/43595

    Abstract: This invention is to provide a photoprotein which binds with a ligand specific for a substance to be detected at a binding ratio of 1:1 such that the luminescence activity is not reduced by binding with the ligand, a conjugate comprising the luminescent photoprotein and ligand, and a substance detection method which employs the conjugate as a marker. A calcium-binding photoprotein is produced having cysteine residue introduced within the 4th amino acid residue from the amino-terminus. A conjugate is formed by binding a ligand specific for a substance to be detected to the calcium-binding photoprotein, in a binding ratio of 1:1, via the introduced cysteine residue. The conjugate may be utilized as a marker for a substance to be detected.

    Abstract translation: 本发明提供一种光蛋白，其以与1:1配体比例的待测物质特异性的配体结合，使得通过与配体的结合不发生发光活性，包含发光光蛋白和配体的缀合物， 以及使用该共轭物作为标记物的物质检测方法。 产生钙结合光蛋白，其在氨基末端的第4个氨基酸残基内引入半胱氨酸残基。 通过引入的半胱氨酸残基以1：1的结合比例将待测物质特异性配体与钙结合光蛋白结合而形成缀合物。 缀合物可以用作待检测物质的标记物。

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