公开(公告)号：US08796027B2

公开(公告)日：2014-08-05

申请号：US11410251

申请日：2006-04-25

申请人： Takeshi Nagasaki ,  Seiji Shinkai ,  Atsushi Uno ,  Mamoru Nishida

发明人： Takeshi Nagasaki ,  Seiji Shinkai ,  Atsushi Uno ,  Mamoru Nishida

IPC分类号： C12N15/00 ,  C12N15/09 ,  A61K38/00 ,  A61K47/00 ,  C12N15/87

CPC分类号： C12N15/87

摘要： A nucleic acid is effectively introduced into a cell while suppressing the cytotoxicity by administering a nucleic acid complex comprising a high molecular weight derivative of ε-poly-L-lysine and a nucleic acid to a cell.

摘要翻译： 通过向细胞施用包含高分子量衍生物和聚-L-赖氨酸和核酸的核酸复合体，将核酸有效地引入细胞同时抑制细胞毒性。

公开(公告)号：US20120319090A1

公开(公告)日：2012-12-20

申请号：US13510178

申请日：2010-11-18

申请人： Seiji Shinkai ,  Shuichi Haraguchi ,  Tomohiro Shirake ,  Masashi Ogawa ,  Shuhei Nakatani ,  Kei Sakanoue ,  Osamu Goto ,  Hidenobu Kakimoto

发明人： Seiji Shinkai ,  Shuichi Haraguchi ,  Tomohiro Shirake ,  Masashi Ogawa ,  Shuhei Nakatani ,  Kei Sakanoue ,  Osamu Goto ,  Hidenobu Kakimoto

IPC分类号： H01L51/30 ,  H01L51/46 ,  H01L51/40 ,  H01L51/54

CPC分类号： H01L51/0512 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1029 ,  G03F7/0046 ,  H01L27/3246 ,  H01L51/0558 ,  H01L51/56 ,  H01L2227/323 ,  H05B33/10

摘要： A problem of the present invention is to provide a device having good characteristics and long life, wherein a functional thin film is formed in a desired region by a coating method; a thin film transistor; a method for producing the device; and a method for producing the thin film transistor. This problem can be solved by a device comprising: a substrate, a first electrode formed on the substrate, a functional thin film formed above the first electrode, and a second electrode disposed above the functional thin film, characterized by further comprising, in a region surrounding the region where the functional thin film is formed, a film containing a compound in which a group containing fluorine and a π-conjugated system are bound together by a cycloalkene structure or a cycloalkane structure.

摘要翻译： 本发明的一个问题是提供一种具有良好特性和长寿命的器件，其中通过涂覆方法在所需区域中形成功能性薄膜; 薄膜晶体管; 一种制造该装置的方法; 以及薄膜晶体管的制造方法。 这个问题可以通过一种器件来解决，该器件包括：衬底，形成在衬底上的第一电极，形成在第一电极上的功能性薄膜，以及设置在功能性薄膜上方的第二电极，其特征在于， 围绕形成功能性薄膜的区域，含有其中含氟基团和共轭体系的基团通过环烯烃结构或环烷烃结构结合在一起的化合物的膜。

公开(公告)号：US20080242854A1

公开(公告)日：2008-10-02

申请号：US11579403

申请日：2006-05-16

申请人： Masami Mizu ,  Seiji Shinkai ,  Teruaki Hasegawa ,  Munenori Numata ,  Tomohisa Fujisawa ,  Kazuo Sakurai

发明人： Masami Mizu ,  Seiji Shinkai ,  Teruaki Hasegawa ,  Munenori Numata ,  Tomohisa Fujisawa ,  Kazuo Sakurai

IPC分类号： C07H1/00 ,  C07H3/00

CPC分类号： C08B37/0024 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/168 ,  C01B2202/02 ,  C08B33/00 ,  C08B37/0057 ,  Y10S977/75 ,  Y10S977/753

摘要： Disclosed is a complex which comprises a carbon nanotube and a modified polysaccharide having a backbone chain with the side thereof being introduced with monosaccharide or oligosaccharide residues. The polysaccharide is preferably β-1,3-glucan. The complex is prepared by admixing a solution of the modified polysaccharide dissolved in an aprotic polar solvent or a strong alkali solution with an aqueous dispersion of the carbon nanotube, and incubating the mixture.

摘要翻译： 公开了一种络合物，其包含碳纳米管和具有主链的改性多糖，其侧面被引入单糖或寡糖残基。 多糖优选为β-1,3-葡聚糖。 通过将溶解在非质子极性溶剂或强碱溶液中的改性多糖的溶液与碳纳米管的水性分散体混合并将该混合物混合来制备络合物。

公开(公告)号：US20060084149A1

公开(公告)日：2006-04-20

申请号：US10471366

申请日：2002-03-11

申请人： Taro Kimura ,  Masami Mizu ,  Kazuo Sakurai ,  Seiji Shinkai ,  Kazuya Koumoto

发明人： Taro Kimura ,  Masami Mizu ,  Kazuo Sakurai ,  Seiji Shinkai ,  Kazuya Koumoto

IPC分类号： A61K48/00 ,  C07H21/04 ,  A61K31/715 ,  C12P19/34

CPC分类号： A61K9/0019 ,  A61K31/711 ,  A61K31/716 ,  A61K47/36 ,  A61K48/0041 ,  C12N15/87

摘要： Disclosed are gene carriers utilizing β-1,3-glucan and methods of preparing the same. The β-1,3-glucan has at least one 1,6-glucopyranoside branch and is chemically modified through periodate oxidation and reductive amination so as to impart nucleic acid-binding functional groups (for example, cationic functional groups) to at least some of the 1,6-glucopyranoside branches thereof. Triple helix β-1,3-glucan is dissolved in a polar organic solvent to form single-stranded β-1,3-glucan. By replacing, in the presence of a nucleic acid, the polar organic solvent for the solution containing the chemically modified single-stranded β-1,3-glucan by water, a complex (gene carrier) is formed in which the nucleic acid is bound to double-stranded β-1,3-glucan.

公开(公告)号：US5412114A

公开(公告)日：1995-05-02

申请号：US15408

申请日：1993-02-09

申请人： Seiji Shinkai ,  Tsutomu Matsuda ,  Takashi Arimura ,  Hirosuke Kawabata ,  Kozo Tachibana

发明人： Seiji Shinkai ,  Tsutomu Matsuda ,  Takashi Arimura ,  Hirosuke Kawabata ,  Kozo Tachibana

IPC分类号： C07B57/00 ,  C07C2/86 ,  C07C13/70 ,  C07C41/01 ,  C07C43/20 ,  C07C43/21 ,  C07D213/30 ,  C07D313/00

CPC分类号： C07D213/30 ,  C07C43/21 ,  C07C2103/92

摘要： The present invention relates to an improvement in the process for preparing a calixarene derivative in which the rotation of its benzene units are hindered and which comprises replacing all or a part of the hydrogen atoms of the hydroxyl groups of a calixarene derivative represented by the general formula: ##STR1## and is characterized by conducting the reaction in the presence of an alkaline earth metal. According to the present invention, an asymmetric calixarene derivative having a "cone" conformation can be selectively prepared by virtue of the template effect of the metal.

摘要翻译： 本发明涉及制备其苯单元旋转受阻的杯芳烃衍生物的制备方法的改进，其包括用通式（I）表示的杯芳烃衍生物的羟基的全部或一部分代替 ：，其特征在于在碱土金属存在下进行反应。 根据本发明，可以通过金属的模板效应选择性地制备具有“锥形”构型的不对称杯芳烃衍生物。

公开(公告)号：US20120330047A1

公开(公告)日：2012-12-27

申请号：US13510207

申请日：2010-11-18

申请人： Seiji Shinkai ,  Shuichi Haraguchi ,  Tomohiro Shiraki ,  Masashi Ogawa ,  Shuhei Nakatani ,  Kei Sakanoue ,  Osamu Goto ,  Hidenobu Kakimoto

发明人： Seiji Shinkai ,  Shuichi Haraguchi ,  Tomohiro Shiraki ,  Masashi Ogawa ,  Shuhei Nakatani ,  Kei Sakanoue ,  Osamu Goto ,  Hidenobu Kakimoto

IPC分类号： C07F7/18

CPC分类号： G03F7/027 ,  C07F7/12 ,  C07F7/1804 ,  G03F7/0046 ,  G03F7/0755 ,  H01L51/0056 ,  H01L51/0095

摘要： A problem of the present invention is to prevent a base layer beneath the layer to be irradiated with light from deterioration in property and a functional thin film from deterioration in property as the fine patterning of a functional film is performed with light irradiation. Means for solving the problem is a compound obtained by dimerizing with light irradiation a compound (A) containing a group that has photosensitivity and can be photodimerized and a group having lyophilicity and a compound (B) containing a group that has photosensitivity and can be photodimerized and a group having liquid-repellency.

摘要翻译： 本发明的问题在于，通过光照射进行功能性薄膜的精细图案化，可以防止层下面的基底层被特性劣化的光照射，功能性薄膜的性能劣化。 用于解决问题的方法是通过光照射包含具有光敏性且可以被光二聚化的基团的化合物（A）和具有亲液性的基团和含有具有光敏性并可以被光二聚化的基团的化合物（B））而获得的化合物 和具有拒水性的组。

公开(公告)号：US20110301230A1

公开(公告)日：2011-12-08

申请号：US13214580

申请日：2011-08-22

申请人： Kazuo SAKURAI ,  Seiji Shinkai ,  Taro Kimura ,  Kengo Tabata ,  Kazuya Koumoto ,  Oliver Gronwald

发明人： Kazuo SAKURAI ,  Seiji Shinkai ,  Taro Kimura ,  Kengo Tabata ,  Kazuya Koumoto ,  Oliver Gronwald

IPC分类号： A61K48/00 ,  C07H21/04 ,  C12N5/00 ,  C07H21/02

CPC分类号： C07H15/04 ,  A61K47/61 ,  A61K47/645 ,  A61K48/00 ,  C07B2200/11 ,  C07H21/00 ,  C08B37/0024 ,  C12N15/87

摘要： Disclosed is a gene carrier composed of a hydrogen-bonding polymer having a polymer chain with hydrogen-bonding sites formed on the polymer chain, in which the polymer chain has a conformation similar to the conformation of a nucleic acid, and particularly, the polymer chain has helix parameters similar to the helix parameters of a nucleic acid of a helix structure. In a preferred embodiment, the hydrogen-bonding polymer is composed of β-1,3-glucan such as schizophyllan. The carrier can be expected to be applied as a vector and a separator for nucleic acids because it is capable of interacting with a nucleic acid to form a complex with the nucleic acid and thus carrying the nucleic acid.

摘要翻译： 公开了一种基因载体，其由具有在聚合物链上形成的氢键位置的聚合物链的氢键聚合物构成，其中聚合物链具有与核酸构象相似的构象，特别是聚合物链 具有类似于螺旋结构的核酸的螺旋参数的螺旋参数。 在一个优选的实施方案中，氢键聚合物由诸如裂枝菌碱等的β-葡聚糖组成。 可以期望载体作为核酸的载体和分离子应用，因为它能够与核酸相互作用以与核酸形成复合物，从而携带核酸。

公开(公告)号：US07790189B2

公开(公告)日：2010-09-07

申请号：US10557108

申请日：2004-05-13

申请人： Masami Mizu ,  Seiji Shinkai ,  Kazuo Sakurai ,  Kazuya Koumoto ,  Munenori Numata ,  Takahiro Matsumoto

发明人： Masami Mizu ,  Seiji Shinkai ,  Kazuo Sakurai ,  Kazuya Koumoto ,  Munenori Numata ,  Takahiro Matsumoto

IPC分类号： A61K45/00 ,  A61K38/16 ,  A61K31/70 ,  A61K31/715 ,  A01N43/04

CPC分类号： A61K9/0019 ,  A61K31/7125 ,  A61K47/36 ,  A61K2039/55561 ,  C08B37/0024

摘要： Disclosed is a new type of immunostimulating agent including an immunostimulating oligonucleotide complexed with a carrier which is safe and has a high transfection effect. The carrier complexed with the immunostimulating oligonucleotide to form the immunostimulating agent is a polysaccharide having β-1,3-bonds (preferably β-1,3-glucan such as schizophyllan). A preferred example of the immunostimulating oligonucleotide is one containing an unmethylated CpG motif. The polysaccharide for use is preferably modified with nucleic acid-binding functional group and/or cell membrane-affinitive functional group.

摘要翻译： 公开了一种新型的免疫刺激剂，其包括与载体复合的免疫刺激寡核苷酸，其是安全的并且具有高的转染效果。 与免疫刺激寡核苷酸复合以形成免疫刺激剂的载体是具有＆bgr; -1,3键的多糖（优选的是诸如分枝杆菌分子-1,3-β-葡聚糖）。 免疫刺激寡核苷酸的优选实例是含有未甲基化CpG基序的寡核苷酸。 所用的多糖优选用核酸结合官能团和/或细胞膜亲和官能团修饰。

公开(公告)号：US20080262210A1

公开(公告)日：2008-10-23

申请号：US10557108

申请日：2004-05-13

申请人： Masami Mizu ,  Seiji Shinkai ,  Kazuo Sakurai ,  Kazuya Koumoto ,  Munenori Numata ,  Takahiro Matsumoto

发明人： Masami Mizu ,  Seiji Shinkai ,  Kazuo Sakurai ,  Kazuya Koumoto ,  Munenori Numata ,  Takahiro Matsumoto

IPC分类号： C07H21/00

CPC分类号： A61K9/0019 ,  A61K31/7125 ,  A61K47/36 ,  A61K2039/55561 ,  C08B37/0024

摘要： Disclosed is a new type of immunostimulating agent including an immunostimulating oligonucleotide complexed with a carrier which is safe and has a high transfection effect. The carrier complexed with the immunostimulating oligonucleotide to form the immunostimulating agent is a polysaccharide having β-1,3-bonds (preferably β-1,3-glucan such as schizophyllan). A preferred example of the immunostimulating oligonucleotide is one containing an unmethylated CpG motif. The polysaccharide for use is preferably modified with nucleic acid-binding functional group and/or cell membrane-affinitive functional group.

摘要翻译： 公开了一种新型的免疫刺激剂，其包括与载体复合的免疫刺激寡核苷酸，其是安全的并且具有高的转染效果。 与免疫刺激寡核苷酸复合以形成免疫刺激剂的载体是具有β-1,3-键的多糖（优选β-1,3-葡聚糖如分枝孢子菌素）。 免疫刺激寡核苷酸的优选实例是含有未甲基化CpG基序的寡核苷酸。 所用的多糖优选用核酸结合官能团和/或细胞膜亲和官能团修饰。

公开(公告)号：US20070281356A1

公开(公告)日：2007-12-06

申请号：US11632138

申请日：2005-07-11

申请人： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

发明人： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

IPC分类号： C12N15/09 ,  C07H21/00 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N15/88 ,  C07K2319/09 ,  C07K2319/23 ,  C12N15/87 ,  C12N2810/6072 ,  C12N2810/85

摘要： To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and/or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.

摘要翻译： 为了提供一种新的技术，通过该技术确保将靶基因递送到细胞中的有效转染，公开了一种用于核进入的核酸构建体，其包含由含有待递送至细胞核的基因的核酸物质组成的三元复合物 的细胞，能够穿过核孔并参与核转运的importin蛋白（例如，importin-β）以及结合核酸物质和进口蛋白的结合物质（例如聚乙烯亚胺） 蛋白。 通过施用与细胞膜受体结合因子和/或膜融合物质结合的核酸构建体，或者将包封在非病毒体内的核酸构建体施用，可以特别地促进从细胞外进入细胞核的核酸 具有细胞膜通透性和膜融合特性的载体（例如仙台病毒包膜）。