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公开(公告)号:US07205397B2
公开(公告)日:2007-04-17
申请号:US09062113
申请日:1998-04-17
申请人: Masaaki Goto , Eisuke Tsuda , Shin′ichi Mochizuki , Kazuki Yano , Fumie Kobayashi , Nobuyuki Shima , Hisataka Yasuda , Nobuaki Nakagawa , Tomonori Morinaga , Masatsugu Ueda , Kanji Higashio
发明人: Masaaki Goto , Eisuke Tsuda , Shin′ichi Mochizuki , Kazuki Yano , Fumie Kobayashi , Nobuyuki Shima , Hisataka Yasuda , Nobuaki Nakagawa , Tomonori Morinaga , Masatsugu Ueda , Kanji Higashio
CPC分类号: C07K14/70578
摘要: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.
摘要翻译: 抑制破骨细胞分化和/或成熟的蛋白质和制备蛋白质的方法。 该蛋白质由人类胚胎肺成纤维细胞产生,在非还原条件下分子量约为60kD,约120kD,SDS-聚丙烯酰胺凝胶电泳时还原条件下分子量约为60kD。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外,蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA,对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。
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公开(公告)号:US07252973B1
公开(公告)日:2007-08-07
申请号:US09647907
申请日:1999-04-09
申请人: Masaaki Goto , Akihiro Tomoyasu , Kazuki Yano , Fumie Kobayashi , Nobuaki Nakagawa , Hisataka Yasuda , Kyoji Yamaguchi , Masahiko Kinosaki , Shin-ichi Mochizuki , Tadashi Nakakarumai , Tomonori Morinaga , Eisuke Tsuda , Kanji Higashio
发明人: Masaaki Goto , Akihiro Tomoyasu , Kazuki Yano , Fumie Kobayashi , Nobuaki Nakagawa , Hisataka Yasuda , Kyoji Yamaguchi , Masahiko Kinosaki , Shin-ichi Mochizuki , Tadashi Nakakarumai , Tomonori Morinaga , Eisuke Tsuda , Kanji Higashio
摘要: A novel protein and a process of producing the protein is provided. The protein is a glycoprotein having activity of suppressing the differentiation and/or maturation of adipocyte, having a molecular weight of about 45 kD under non-reducing conditions and about 28 kD and/or 23 kD under reducing conditions, and exhibiting affinity to heparin. A process of producing the protein comprising culturing human fibroblasts and purifying the culture broth by chromatography using an ion exchange column, affinity column, and reverse phase column.A cDNA encoding the protein and a process of producing the protein using the cDNA are also provided. The protein of the present invention is useful as a pharmaceutical composition for preventing or treating obesity or as an antigen for establishing immunological diagnosis, etc.
摘要翻译: 提供了一种新的蛋白质和产生该蛋白质的方法。 蛋白质是具有抑制脂肪细胞分化和/或成熟的活性的糖蛋白,在还原条件下,分子量约为45kD,在还原条件下具有约28kD和/或23kD,并且对肝素具有亲和力。 一种生产蛋白质的方法,包括培养人成纤维细胞,并使用离子交换柱,亲和柱和反相柱通过色谱法纯化培养液。 还提供了编码蛋白质的cDNA和使用cDNA产生蛋白质的方法。 本发明的蛋白质可用作预防或治疗肥胖症或用于建立免疫诊断等的抗原的药物组合物。
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公开(公告)号:US20050026253A1
公开(公告)日:2005-02-03
申请号:US10900423
申请日:2004-07-28
申请人: Masaaki Goto , Akihiro Tomoyasu , Kazuki Yano , Fumie Kobayashi , Nobuaki Nakagawa , Hisataka Yasuda , Kyoji Yamaguchi , Masahiko Kinosaki , Shin-ichi Mochizuki , Tadashi Nakakarumai , Tomonori Morinaga , Eisuke Tsuda , Kanji Higashio
发明人: Masaaki Goto , Akihiro Tomoyasu , Kazuki Yano , Fumie Kobayashi , Nobuaki Nakagawa , Hisataka Yasuda , Kyoji Yamaguchi , Masahiko Kinosaki , Shin-ichi Mochizuki , Tadashi Nakakarumai , Tomonori Morinaga , Eisuke Tsuda , Kanji Higashio
摘要: A novel protein and a process of producing the protein is provided. The protein is a glycoprotein having activity of suppressing the differentiation and/or maturation of adipocyte, having a molecular weight of about 45 kD under non-reducing conditions and about 28 kD and/or 23 kD under reducing conditions, and exhibiting affinity to heparin. A process of producing the protein comprising culturing human fibroblasts and purifying the culture broth by chromatography using an ion exchange column, affinity column, and reverse phase column. A cDNA encoding the protein and a process of producing the protein using the cDNA are also provided. The protein of the present invention is useful as a pharmaceutical composition for preventing or treating obesity or as an antigen for establishing immunological diagnosis, etc.,
摘要翻译: 提供了一种新的蛋白质和产生该蛋白质的方法。 蛋白质是具有抑制脂肪细胞分化和/或成熟的活性的糖蛋白,在还原条件下,分子量约为45kD,在还原条件下具有约28kD和/或23kD,并且对肝素具有亲和力。 一种生产蛋白质的方法,包括培养人成纤维细胞,并使用离子交换柱,亲和柱和反相柱通过色谱法纯化培养液。 还提供了编码蛋白质的cDNA和使用cDNA产生蛋白质的方法。 本发明的蛋白质可用作预防或治疗肥胖的药物组合物或用作建立免疫诊断等的抗原,
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