Cell lysis by heating-cooling process through endothermic reaction
    2.
    发明授权
    Cell lysis by heating-cooling process through endothermic reaction 有权
    通过吸热反应通过加热 - 冷却过程进行细胞裂解

    公开(公告)号:US07521246B2

    公开(公告)日:2009-04-21

    申请号:US11335414

    申请日:2006-01-19

    IPC分类号: G01N33/48

    摘要: Provided is a cell lysis method including: preparing a cell sample to be lysed; heating the cell sample; and cooling the cell sample by causing an endothermic reaction near the cell sample. According to the method, cell lysis can be simply and conveniently performed without regard to location and without additional devices since a separate energy source is not required and the apparatus is portable. In particular, when cell lysis is performed in a biochip using a small amount of sample, a greater cell lysis effect can be obtained. In addition, cell lysis efficiency is significantly improved, compared to when only heating is performed.

    摘要翻译: 提供了一种细胞裂解方法,包括:制备待裂解的细胞样品; 加热细胞样品; 并通过在细胞样品附近引起吸热反应来冷却细胞样品。 根据该方法,可以简单且方便地进行细胞裂解,而不考虑位置和不需要额外的装置,因为不需要单独的能源并且该装置是便携式的。 特别是,在使用少量样品的生物芯片中进行细胞裂解时,可以获得更大的细胞裂解效果。 此外,与仅进行加热时相比,细胞裂解效率显着提高。

    Method of purifying nucleic acid using silver nanoparticles
    7.
    发明授权
    Method of purifying nucleic acid using silver nanoparticles 失效
    使用银纳米粒子纯化核酸的方法

    公开(公告)号:US07364857B2

    公开(公告)日:2008-04-29

    申请号:US11257833

    申请日:2005-10-25

    IPC分类号: C12Q1/68

    摘要: Provided is a method of purifying a target substance using silver nanoparticles. The method includes: mixing a sample containing molecules having a thiol group with the silver nanoparticles to obtain a complex of the molecules having the thiol group with the silver nanoparticles; and isolating and removing the complex from the mixture. By using the purification method, PCR amplifiable DNAs can be rapidly purified, and thus, the method can be very efficiently applied to lab-on-chip (LOC).

    摘要翻译: 提供了使用银纳米粒子来净化目标物质的方法。 该方法包括:将含有硫醇基的分子的样品与银纳米颗粒混合,得到具有硫醇基的分子与银纳米颗粒的络合物; 并从混合物中分离并除去络合物。 通过使用纯化方法,可以快速纯化PCR扩增的DNA,因此该方法可以非常有效地应用于片上实验(LOC)。

    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex
    8.
    发明申请
    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex 审中-公开
    使用固定化金属 - 配体复合物纯化核酸的方法和设备

    公开(公告)号:US20060275815A1

    公开(公告)日:2006-12-07

    申请号:US11446795

    申请日:2006-06-05

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C07H21/04 C12N15/1006

    摘要: Provided is a method of purifying nucleic acids using a metal-ligand complex, the method comprising: immobilizing the metal-ligand complex on a solid support; bringing a sample containing the nucleic acid into contact with the immobilized complex on the solid support to bind the nucleic acid to the complex; and adding a solution containing a chelate capable of removing the metal to elute the nucleic acid bound to the complex. The nucleic acids can be efficiently purified using a metal-ligand complex interacting with a base of the nucleic acid instead of interacting with a phosphoric acid of the nucleic acid to selectively bind the nucleic acid, and using a chelator capable of removing the metal to elute the nucleic acid.

    摘要翻译: 提供了使用金属 - 配体络合物纯化核酸的方法,所述方法包括:将所述金属 - 配体复合物固定在固体支持物上; 使含有核酸的样品与固体支持物上的固定化复合物接触以将核酸结合到复合物上; 并加入含有能除去金属的螯合物的溶液以洗脱与络合物结合的核酸。 可以使用与核酸的碱基相互作用的金属 - 配体复合物来有效纯化核酸,而不是与核酸的磷酸相互作用以选择性地结合核酸,并且使用能够去除金属洗脱的螯合剂 核酸。