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公开(公告)号：US10160867B2
公开(公告)日：2018-12-25
申请号：US15227154
申请日：2016-08-03
Applicant: Stephen J. Benkovic , Chunyu Liu , Edward Q. Kaiser
Inventor： Stephen J. Benkovic , Chunyu Liu , Edward Q. Kaiser
Abstract: A latex paint composition that contains a film-forming binder and pigment dispersed in an aqueous vehicle and also contains an effective amount of one or more cellulose-supportable fungus growth-inhibiting benzoxaborole compounds of Formula C is disclosed. A method of using that latex paint to inhibit the growth of a cellulose-supportable fungus on a cellulosic surface is also disclosed, as is a method of inhibiting such growth by painting over an fungus-infected surface with a contemplated latex paint.
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公开(公告)号：US20140356936A1
公开(公告)日：2014-12-04
申请号：US14313193
申请日：2014-06-24
Applicant: Stephen J BENKOVIC , Frank Salinas
Inventor： Stephen J BENKOVIC , Frank Salinas
IPC: C12Q1/68
CPC classification number: C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
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公开(公告)号：US20140234910A1
公开(公告)日：2014-08-21
申请号：US14268444
申请日：2014-05-02
Applicant: Stephen J. BENKOVIC , Frank Salinas
Inventor： Stephen J. BENKOVIC , Frank Salinas
IPC: C12Q1/68
CPC classification number: C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
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公开(公告)号：US08673561B2
公开(公告)日：2014-03-18
申请号：US13722617
申请日：2012-12-20
Applicant: Stephen J Benkovic , Frank Salinas
Inventor： Stephen J Benkovic , Frank Salinas
CPC classification number: C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
Abstract translation: 描述了用于复制和扩增靶核酸序列的方法。 本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。 用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。 在优选的实施方案中，聚合酶包含聚合酶全酶。 在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白 。
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公开(公告)号：US20130330776A2
公开(公告)日：2013-12-12
申请号：US13722765
申请日：2012-12-20
Applicant: Stephen J BENKOVIC , Frank SALINAS
Inventor： Stephen J BENKOVIC , Frank SALINAS
IPC: C12P19/34
CPC classification number: C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
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公开(公告)号：US07465836B2
公开(公告)日：2008-12-16
申请号：US10868268
申请日：2004-06-15
Applicant: Ving Lee , Jacob J. Plattner , Stephen J. Benkovic , Stephen J. Baker , Kirk R. Maples , Carolyn Bellinger-Kawahara , Tsutomu Akama , Yong-Kang Zhang , Rajeshwar Singh , Vittorio A. Sauro
Inventor： Ving Lee , Jacob J. Plattner , Stephen J. Benkovic , Stephen J. Baker , Kirk R. Maples , Carolyn Bellinger-Kawahara , Tsutomu Akama , Yong-Kang Zhang , Rajeshwar Singh , Vittorio A. Sauro
IPC: C07F9/02
CPC classification number: C07F5/025
Abstract: Compositions and methods of use of borole derivatives, including benzoxaboroles, benzazaboroles and benzthiaboroles, as therapeutic agents for treatment of diseases caused by bacteria or viruses are disclosed, as well as methods for synthesis of said agents and compositions thereof.
Abstract translation: 公开了用于治疗由细菌或病毒引起的疾病的治疗剂的硼烷衍生物，包括苯并恶唑，苯并唑并苯并噻唑的组合物和方法，以及合成所述试剂及其组合物的方法。
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公开(公告)号：US20080227659A1
公开(公告)日：2008-09-18
申请号：US12033672
申请日：2008-02-19
Applicant: Stephen J. Benkovic , Marc Ostermeier , Stefan Lutz , Andrew E. Nixon
Inventor： Stephen J. Benkovic , Marc Ostermeier , Stefan Lutz , Andrew E. Nixon
CPC classification number: C12N15/102 , C12N15/1027 , C12N15/1058
Abstract: A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology, is also described. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides.
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公开(公告)号：US06929915B2
公开(公告)日：2005-08-16
申请号：US10125973
申请日：2002-04-19
Applicant: Stephen J. Benkovic , Frank Salinas
Inventor： Stephen J. Benkovic , Frank Salinas
CPC classification number: C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
Abstract translation: 描述了用于复制和扩增靶核酸序列的方法。 本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。 用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。 在优选的实施方案中，聚合酶包含聚合酶全酶。 在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白 。
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公开(公告)号：US5834195A
公开(公告)日：1998-11-10
申请号：US718428
申请日：1996-12-18
Applicant: Stephen J. Benkovic , Nicholas Winograd , Christopher L. Brummel , Irene N. W. Lee
Inventor： Stephen J. Benkovic , Nicholas Winograd , Christopher L. Brummel , Irene N. W. Lee
IPC: C07D487/04 , C07H21/00 , C07K1/04 , G01N33/53 , C12Q1/68
CPC classification number: C07H21/00 , C07K1/047 , B01J2219/00702 , Y10T436/24
Abstract: A method to determine the molecular weights of femtomole or smaller quantities of small molecules, such as peptides, oligonucleotides, or heterocyclics, covalently attached to polystyrene beads on a grid, is presented using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS). The determination is made possible by selectively clipping the bond linking the small molecule to the bead, followed directly by a TOF-SIMS assay of the bead on the grid. The method can be applied to large numbers of polystyrene beads having different small molecules attached thereto for direct characterization of massive combinatorial libraries.
Abstract translation: PCT No.PCT / US95 / 03355 Sec。 371日期：1996年12月18日 102（e）日期1996年12月18日PCT提交1995年3月23日PCT公布。 公开号WO95 / 25737 日期1995年9月28日使用成像飞行时间二次离子来确定飞甲托或分子量较小的小分子如分子量，如肽，寡核苷酸或杂环的共价连接到网格上的聚苯乙烯珠粒的方法 质谱（TOF-SIMS）。 通过选择性地剪切将小分子连接到珠粒上的键，然后直接通过网格上的珠粒的TOF-SIMS测定来确定。 该方法可以应用于大量具有连接到其上的不同小分子的聚苯乙烯珠，用于大规模组合文库的直接表征。
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公开(公告)号：US5665865A
公开(公告)日：1997-09-09
申请号：US343658
申请日：1994-11-22
Applicant: Richard A. Lerner , Victoria A. Roberts , Elizabeth D. Getzoff , John A. Tainer , Stephen J. Benkovic
Inventor： Richard A. Lerner , Victoria A. Roberts , Elizabeth D. Getzoff , John A. Tainer , Stephen J. Benkovic
IPC: A61K39/395 , C07K14/00 , C07K16/00 , C07K19/00 , C12N1/21 , C12N9/00 , C12N15/09 , C12P21/00 , C12P21/08 , C12R1/19 , G01N33/53
CPC classification number: C12N9/0002 , C07K16/00
Abstract: The invention describes a metal binding protein capable of forming a coordination complex with a metal cation. The protein contains a sequence of amino acid residues that defines a variable domain of an immunoglobulin light chain having a L1 region and a L3 region, and also contains three contact amino acid residues in the variable domain that participate as ligands for the metal coordination complex.
Abstract translation: 本发明描述了能够与金属阳离子形成配位络合物的金属结合蛋白。 蛋白质含有限定具有L1区域和L3区域的免疫球蛋白轻链可变结构域的氨基酸残基序列，并且在可变结构域中还含有参与作为金属配位络合物配体的三个接触氨基酸残基。
