利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12 条记录 (耗时 0.022 秒)

    ATOMIC FORCE MICROSCOPE AS AN ANALYZING TOOL FOR BIOCHIP
    4.
    发明申请
    ATOMIC FORCE MICROSCOPE AS AN ANALYZING TOOL FOR BIOCHIP 审中-公开
    原子力显微镜作为生物技术分析工具

    公开(公告)号：US20100261615A9

    公开(公告)日：2010-10-14

    申请号：US12140226

    申请日：2008-06-16

    申请人： Joon Won Park ,  Bong Jin Hong ,  Sung Hong Kwon

    发明人： Joon Won Park ,  Bong Jin Hong ,  Sung Hong Kwon

    IPC分类号： C40B30/10 ,  C40B60/12

    CPC分类号： G01N33/6845 ,  G01Q60/42

    摘要： The present application discloses a method for detecting a presence of target ligand in a fluid medium which includes the steps of: (i) contacting the fluid medium with a solid substrate that includes an array of dendrons on its surface, wherein each of the dendron includes a central atom, a probe that is attached to the central atom optionally through a linker, and a base portion attached to the central atom and having a plurality of termini that are attached to the surface of the solid support; and (ii) determining the presence of a probe-target ligand complex by measuring binding force between the bound ligand and detection molecule tethered to the tip of an atomic force microscope (“AFM”), which detection molecule has affinity for the ligand, wherein measurement of an increase in force between the probe-target ligand complex and the detection molecule by AFM indicates the presence of the probe-target ligand complex.

    摘要翻译： 本申请公开了一种用于检测流体介质中目标配体存在的方法，其包括以下步骤：（i）使流体介质与其表面上包含一系列树突的固体基质接触，其中每个树枝包括 中心原子，任选地通过连接体连接到中心原子上的探针和连接到中心原子上并具有连接到固体载体的表面上的多个末端的基部; 和（ii）通过测量结合的配体和与原子力显微镜（“AFM”）的末端连接的检测分子之间的结合力来确定探针 - 靶配体复合物的存在，该检测分子对配体具有亲和性，其中 通过AFM测量探针 - 靶配体复合物和检测分子之间的力的增加表明探针 - 靶配体复合物的存在。

    ATOMIC FORCE MICROSCOPE AS AN ANALYZING TOOL FOR BIOCHIP
    5.
    发明申请
    ATOMIC FORCE MICROSCOPE AS AN ANALYZING TOOL FOR BIOCHIP 审中-公开
    原子力显微镜作为生物技术分析工具

    公开(公告)号：US20090048120A1

    公开(公告)日：2009-02-19

    申请号：US12140226

    申请日：2008-06-16

    申请人： Joon Won Park ,  Bong Jin Hong ,  Sung Hong Kwon

    发明人： Joon Won Park ,  Bong Jin Hong ,  Sung Hong Kwon

    IPC分类号： C40B30/10 ,  C40B60/12

    CPC分类号： G01N33/6845 ,  G01Q60/42

    摘要： The present application discloses a method for detecting a presence of target ligand in a fluid medium which includes the steps of: (i) contacting the fluid medium with a solid substrate that includes an array of dendrons on its surface, wherein each of the dendron includes a central atom, a probe that is attached to the central atom optionally through a linker, and a base portion attached to the central atom and having a plurality of termini that are attached to the surface of the solid support; and (ii) determining the presence of a probe-target ligand complex by measuring binding force between the bound ligand and detection molecule tethered to the tip of an atomic force microscope (“AFM”), which detection molecule has affinity for the ligand, wherein measurement of an increase in force between the probe-target ligand complex and the detection molecule by AFM indicates the presence of the probe-target ligand complex.

    摘要翻译： 本申请公开了一种用于检测流体介质中目标配体存在的方法，其包括以下步骤：（i）使流体介质与其表面上包含一系列树突的固体基质接触，其中每个树枝包括 中心原子，任选地通过连接体连接到中心原子上的探针和连接到中心原子上并具有连接到固体载体的表面上的多个末端的基部; 和（ii）通过测量结合的配体和与原子力显微镜（“AFM”）的末端连接的检测分子之间的结合力来确定探针 - 靶配体复合物的存在，该检测分子对配体具有亲和性，其中 通过AFM测量探针 - 靶配体复合物和检测分子之间的力的增加表明探针 - 靶配体复合物的存在。

    CELL LYSIS DEVICE AND METHODS OF LYSING CELLS OR VIRUSES
    9.
    发明申请
    CELL LYSIS DEVICE AND METHODS OF LYSING CELLS OR VIRUSES 有权
    细胞检测装置和遗传细胞或病毒的方法

    公开(公告)号：US20120107912A1

    公开(公告)日：2012-05-03

    申请号：US13284364

    申请日：2011-10-28

    申请人： Kyu-youn HWANG ,  Joon-ho KIM ,  Sung-hong KWON ,  Chin-sung PARK ,  Hee-kyun LIM ,  Sun-ok JUNG ,  Won-jong JUNG

    发明人： Kyu-youn HWANG ,  Joon-ho KIM ,  Sung-hong KWON ,  Chin-sung PARK ,  Hee-kyun LIM ,  Sun-ok JUNG ,  Won-jong JUNG

    IPC分类号： C12M1/00 ,  C12N1/20 ,  C12N5/04 ,  C12N1/14 ,  C12N7/00 ,  C12N5/07

    CPC分类号： C12M1/00 ,  C12M1/33 ,  C12M47/06

    摘要： A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.

    摘要翻译： 一种裂解细胞和病毒中的至少一种的方法，所述方法包括：将包括细胞和病毒中的至少一种的样品与设置在第一室中的多个珠接触以获得组合 的样品和珠粒; 并搅拌样品和珠的组合以裂解细胞和病毒中的至少一种，其中在第一室中液体体积分数为0.6或更小，并且其中液体体积分数是通过将 第一室的液体体积相当于第一室的液体体积和第一室的空隙体积的总和的纯空隙体积。

    Cell lysis device and methods of lysing cells or viruses
    10.
    发明授权
    Cell lysis device and methods of lysing cells or viruses 有权
    细胞裂解装置和裂解细胞或病毒的方法

    公开(公告)号：US08986986B2

    公开(公告)日：2015-03-24

    申请号：US13284364

    申请日：2011-10-28

    申请人： Kyu-youn Hwang ,  Joon-ho Kim ,  Sung-hong Kwon ,  Chin-sung Park ,  Hee-kyun Lim ,  Sun-ok Jung ,  Won-jong Jung

    发明人： Kyu-youn Hwang ,  Joon-ho Kim ,  Sung-hong Kwon ,  Chin-sung Park ,  Hee-kyun Lim ,  Sun-ok Jung ,  Won-jong Jung

    IPC分类号： C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  C12Q1/70 ,  C12M3/00 ,  C12M1/00

    CPC分类号： C12M1/00 ,  C12M1/33 ,  C12M47/06

    摘要： A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.

    摘要翻译： 一种裂解细胞和病毒中的至少一种的方法，所述方法包括：将包括细胞和病毒中的至少一种的样品与设置在第一室中的多个珠接触以获得组合 的样品和珠粒; 并搅拌样品和珠的组合以裂解细胞和病毒中的至少一种，其中在第一室中液体体积分数为0.6或更小，并且其中液体体积分数是通过将 第一室的液体体积相当于第一室的液体体积和第一室的空隙体积的总和的纯空隙体积。