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公开(公告)号：US20090055969A1
公开(公告)日：2009-02-26
申请号：US12166239
申请日：2008-07-01
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.
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2.发明授权Genes encoding novel proteins with pesticidal activity against coleopterans 有权编码对鞘翅目具有杀虫活性的新型蛋白质的基因公开(公告)号：US08188036B2
公开(公告)日：2012-05-29
申请号：US12472997
申请日：2009-05-27
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
IPC分类号： A01N37/18
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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公开(公告)号：US07803925B2
公开(公告)日：2010-09-28
申请号：US12166239
申请日：2008-07-01
IPC分类号： C07H21/04 , C12P21/02 , A01N33/00 , A61K31/7052
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.
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4.发明申请公开(公告)号：US20090291896A1
公开(公告)日：2009-11-26
申请号：US12472997
申请日：2009-05-27
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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5.发明授权Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans 有权编码具有抗鞘翅目杀虫活性的新型苏云金芽孢杆菌蛋白的基因公开(公告)号：US07605304B2
公开(公告)日：2009-10-20
申请号：US10032717
申请日：2001-10-23
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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6.发明申请AXMI-028 AND AXMI-029, A FAMILY OF NOVEL DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权AXMI-028和AXMI-029，一种新的内啡肽基因家族及其使用方法公开(公告)号：US20090054335A1
公开(公告)日：2009-02-26
申请号：US12166253
申请日：2008-07-01
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。
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公开(公告)号：US07105332B2
公开(公告)日：2006-09-12
申请号：US11108389
申请日：2005-04-18
申请人： André Abad , Ronald D. Flannagan , Rafael Herrmann , Theodore W. Kahn , Albert L. Lu , Billy F. McCutchen , James K. Presnail , James F. H. Wong , Cao-Guo Yu
发明人： André Abad , Ronald D. Flannagan , Rafael Herrmann , Theodore W. Kahn , Albert L. Lu , Billy F. McCutchen , James K. Presnail , James F. H. Wong , Cao-Guo Yu
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编码具有杀虫活性的昆虫害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。 本发明进一步提供已被修饰以编码杀虫多肽的诱变核酸，例如具有改善的杀虫活性和/或改变的有害生物特异性的内毒素。 本发明的具体实施方案提供编码可能被优化的杀虫蛋白质的分离的核酸，以及包含本发明的核酸的农药组合物，表达盒和转化的微生物和植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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8.发明授权Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans 有权编码具有杀虫活性的新型苏云金芽孢杆菌蛋白的基因公开(公告)号：US07696412B2
公开(公告)日：2010-04-13
申请号：US10414637
申请日：2003-04-16
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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