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    Method for creating perfusable microvessel systems
    1.
    发明授权
    Method for creating perfusable microvessel systems 有权
    创建可灌注微血管系统的方法

    公开(公告)号：US09446169B2

    公开(公告)日：2016-09-20

    申请号：US13212107

    申请日：2011-08-17

    Applicant: Thomas Neumann

    Inventor： Thomas Neumann

    IPC: A61L27/38 ,  C12M3/00 ,  C12M1/00 ,  C12N5/00 ,  C12N5/071 ,  C12M1/12

    CPC classification number: A61L27/3808 ,  C12M21/08 ,  C12M23/06 ,  C12M23/20 ,  C12M25/14 ,  C12M29/10 ,  C12N5/0068 ,  C12N5/0691 ,  C12N2533/50

    Abstract: A method for the creation of endothelial parent vessels from human vascular endothelial cells in vitro in a culture perfusion device (CPD) including a collagen chamber, inlet ports, a capillary tube, and an outlet port. A collagen solution is injected into the collagen chamber through a syringe needle until the chamber is filled with collagen. The CPD is perfused by filling the inlet ports and sequentially priming the inlet ports, and the outlet ports. A perfusable channel is created in the collagen chamber and a concentrated suspension of endothelial cells is injected into the inlet ports. The endothelial cells are injected into the at least one perfusable channel and incubated to attach to the walls of the perfusable channel. The cells are distributed within the CPD; and perfused to form a parent vessel having homogeneous monolayers of cells.

    Abstract translation: 一种用于在包括胶原室，入口，毛细管和出口的培养灌注装置（CPD）中体外从人血管内皮细胞产生内皮亲本血管的方法。 胶原溶液通过注射器针头注射到胶原室中，直到腔室充满胶原蛋白。 通过填充入口并依次灌注入口和出口来灌注CPD。 在胶原蛋白室中产生可灌注通道，并将内皮细胞的浓缩悬浮液注入入口。 将内皮细胞注射到至少一个可灌注通道中并孵育以附着到可灌注通道的壁上。 细胞分布在CPD内; 并灌注以形成具有均匀单层细胞的亲本血管。

    PROCESS FOR REACTING HYDROXYL COMPOUNDS WITH LINEAR, BRANCHED OR CYCLIC POLYALKYLSILOXANES
    3.
    发明申请
    PROCESS FOR REACTING HYDROXYL COMPOUNDS WITH LINEAR, BRANCHED OR CYCLIC POLYALKYLSILOXANES 审中-公开
    用直链，分支或环状聚烷氧基硅烷反应羟基化合物的方法

    公开(公告)号：US20120142956A1

    公开(公告)日：2012-06-07

    申请号：US13306439

    申请日：2011-11-29

    Applicant: Thomas Neumann

    Inventor： Thomas Neumann

    IPC: C07F7/18

    CPC classification number: C08G77/44 ,  C08G77/08

    Abstract: A process for preparing organopolysiloxanes by reacting linear siloxane compounds (II) and/or cyclic siloxane compounds (III) with a compound having at least one hydroxyl group R′—OH (IV) where R′ are identical or different organic radicals, which is characterized in that the compounds of the formulae (II), (III) and (IV) are used in such amounts that the molar ratio of silicon atoms in the compounds of the formulae (II) and (III) to OH groups in the compounds of the formula (IV) is from 0.01:1 to 1000:1, and in that the reaction is performed at a temperature of greater than 70° C. to 175° C., and in that the resulting reaction mixture is not treated with an acidic compound.

    Abstract translation: 通过使直链硅氧烷化合物（II）和/或环状硅氧烷化合物（III）与具有至少一个羟基R'-OH（IV）的化合物反应制备有机聚硅氧烷的方法，其中R'是相同或不同的有机基团，其是 其特征在于，式（II），（III）和（IV）的化合物的用量使得式（II）和（III）的化合物中的硅原子与化合物中的OH基的摩尔比 的式（Ⅳ）为0.01:1至1000:1，反应在大于70℃至175℃的温度下进行，并且所得反应混合物不用 酸性化合物。

    Process for reacting polyorganosiloxanes and their use
    4.
    发明授权
    Process for reacting polyorganosiloxanes and their use 有权
    聚有机硅氧烷的反应及其用途

    公开(公告)号：US07825206B2

    公开(公告)日：2010-11-02

    申请号：US11956525

    申请日：2007-12-14

    Applicant: Thomas Neumann ,  Wilfried Knott

    Inventor： Thomas Neumann ,  Wilfried Knott

    IPC: C08G77/18

    CPC classification number: C08G77/08 ,  C08G77/16 ,  C08G77/42

    Abstract: The invention provides a process for preparing SiOC-bonded polyorganosiloxanes by reacting, by processes known per se, hydroxyl-containing compounds with a stoichiometric excess of polyorganosiloxanes which contain —Si(H) units and are of the general formula (I) in the presence of one or more element compounds of main group III and/or of transition group 3 as a catalyst, wherein the reaction, on completion of conversion of the compounds containing hydroxyl groups, is continued until no further ≡Si—H groups are detectable by gas volumetric means, and also the compounds prepared in this way and their use.

    Abstract translation: 本发明提供了一种制备SiOC键合的聚有机硅氧烷的方法，其通过本身已知的方法使含羟基化合物与含有-Si（H）单元的化学计量过量的聚有机硅氧烷在通式（I）的存在下反应， 的主要III族和/或过渡族3的元素化合物作为催化剂，其中在含羟基的化合物的转化完成时的反应继续进行，直到不再有气体可以检测到≡Si-H基团 体积的方法，以及以这种方式制备的化合物及其用途。

    METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS
    5.
    发明申请
    METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS 有权
    创造可靠的微电子系统的方法

    公开(公告)号：US20090317787A1

    公开(公告)日：2009-12-24

    申请号：US12541707

    申请日：2009-08-14

    Applicant: Thomas Neumann

    Inventor： Thomas Neumann

    IPC: A01N1/02 ,  C12N5/08

    CPC classification number: C12N5/0691 ,  C12M21/08 ,  C12M23/06 ,  C12M23/16 ,  C12M23/20 ,  C12M29/10 ,  C12N2533/50

    Abstract: Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused.

    Abstract translation: 在体外创建组织结构包括在培养/灌注装置框架上并置心轴，其中心轴彼此间隔开并且将心轴连接到包括上游管和下游管的管。 上游管与上游歧管连接，下游管连接到下游歧管。 框架和心轴被消毒，涂覆并种植有细胞，每个细胞在每个心轴周围形成圆形层，直到圆形层合并成经历生长培养基的组织结构。 提取心轴并灌注组织结构。

    PROCESS FOR THE TREATMENT OF SiC-BONDED POLYETHERSILOXANES
    7.
    发明申请
    PROCESS FOR THE TREATMENT OF SiC-BONDED POLYETHERSILOXANES 审中-公开
    用于处理SiC结合的聚乙二醇的方法

    公开(公告)号：US20080227923A1

    公开(公告)日：2008-09-18

    申请号：US11755441

    申请日：2007-05-30

    Applicant: Klaus-Deiter Klein ,  Thomas Neumann

    Inventor： Klaus-Deiter Klein ,  Thomas Neumann

    IPC: C08F8/04

    CPC classification number: C08G77/46 ,  C08G77/34

    Abstract: The invention relates to a process for the treatment of polyoxyalkylene-polysiloxane block copolymers or alkylpolysiloxane-polyoxyalkylenepolysiloxane block copolymers in which the polysiloxane blocks are bonded to the polyether blocks or alkyl radicals by SiC bonds, wherein the polyoxyalkylene-polysiloxane block copolymers or alkylpolysiloxane-polyoxyalkylenepolysiloxane block copolymers or the solutions thereof are treated with flowing hydrogen gas and optionally a further inert gas in the presence of a combination of hydrogenation catalysts known per se and acid-activated carrier materials and water at temperatures of from 20 to 200° C. and atmospheric pressure over a period of from 0.5 to 10 hours.

    Abstract translation: 本发明涉及一种用于处理聚氧化烯 - 聚硅氧烷嵌段共聚物或烷基聚硅氧烷 - 聚氧亚烷基聚硅氧烷嵌段共聚物的方法，其中聚硅氧烷嵌段通过SiC键与聚醚嵌段或烷基键合，其中聚氧化烯 - 聚硅氧烷嵌段共聚物或烷基聚硅氧烷 - 聚氧亚烷基聚硅氧烷 嵌段共聚物或其溶液在流动的氢气和任选的另外的惰性气体下，在本身已知的氢化催化剂和酸活化的载体材料和水的温度下，在20〜200℃的温度和大气压下进行处理 压力为0.5〜10小时。

    Method for creating perfusable microvessel systems
    8.
    发明申请
    Method for creating perfusable microvessel systems 有权
    创建可灌注微血管系统的方法

    公开(公告)号：US20070224677A1

    公开(公告)日：2007-09-27

    申请号：US11388920

    申请日：2006-03-24

    Applicant: Thomas Neumann

    Inventor： Thomas Neumann

    IPC: C12N5/08 ,  C12M3/00 ,  C12N5/06

    CPC classification number: C12N5/0691 ,  C12M21/08 ,  C12M23/06 ,  C12M23/16 ,  C12M23/20 ,  C12M29/10 ,  C12N2533/50

    Abstract: Microvessel networks are produced in vitro from tissue-engineered parent vessels sprouting into a supporting matrix, as for example gels, of extracellular matrix proteins. The microvessel systems are integrated into devices that allow for controlled perfusion with fluids. The vessels may include cells from one cell type, for example, endothelial cells, or from combinations of two or more cell types.

    Abstract translation: 微血管网络在体外从组织工程化的母体血管发育成支持基质，例如凝胶，细胞外基质蛋白。 微血管系统集成到允许用流体控制灌注的装置中。 血管可以包括来自一种细胞类型的细胞，例如内皮细胞，或两种或多种细胞类型的组合。

    Method for characterizing polypeptides
    9.
    发明授权
    Method for characterizing polypeptides 失效
    表征多肽的方法

    公开(公告)号：US07163803B2

    公开(公告)日：2007-01-16

    申请号：US10479843

    申请日：2002-06-07

    Applicant: Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

    Inventor： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

    IPC: C12Q1/37

    CPC classification number: G01N33/6848 ,  C07K1/12 ,  G01N33/6821

    Abstract: Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.

    Abstract translation: 提供了表征多肽的方法，该方法包括以下步骤：（a）任选地减少多肽中的半胱氨酸二硫键形成游离硫醇，并封端游离硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

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