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    Lactobacillus acidophilus nucleic acid sequences encoding protease homologues and uses therefore
    3.
    发明授权
    Lactobacillus acidophilus nucleic acid sequences encoding protease homologues and uses therefore 有权
    因此，编码蛋白酶同源物的嗜酸乳杆菌核酸序列和用途

    公开(公告)号：US07897367B2

    公开(公告)日：2011-03-01

    申请号：US12251501

    申请日：2008-10-15

    申请人： Todd R. Klaenhammer ,  Eric Altermann ,  W. Michael Russell

    发明人： Todd R. Klaenhammer ,  Eric Altermann ,  W. Michael Russell

    IPC分类号： C12P21/06

    CPC分类号： C12N9/50 ,  C07K14/335

    摘要： Protease-like nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, protease-like fusion proteins, antigenic peptides, and anti-protease-like antibodies are encompassed. The invention also provides vectors containing a nucleic acid molecule of the invention and cells into which the vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.

    摘要翻译： 蛋白酶样核酸分子及其多肽及其片段和变体在本发明中公开。 此外，还包括蛋白酶样融合蛋白，抗原肽和抗蛋白酶样抗体。 本发明还提供了含有本发明的核酸分子的载体和已经引入载体的细胞。 进一步公开了制备多肽的方法和本发明多肽的使用方法。

    Lactobacillus acidophilus nucleic acids encoding fructo-oligosaccharide utilization compounds and uses thereof
    4.
    发明授权
    Lactobacillus acidophilus nucleic acids encoding fructo-oligosaccharide utilization compounds and uses thereof 失效
    编码低聚果糖的化合物的嗜酸乳酸乳杆菌核酸及其用途

    公开(公告)号：US07407787B2

    公开(公告)日：2008-08-05

    申请号：US10873467

    申请日：2004-06-22

    申请人： Rodolphe Barrangou ,  Todd R. Klaenhammer ,  Eric Altermann

    发明人： Rodolphe Barrangou ,  Todd R. Klaenhammer ,  Eric Altermann

    IPC分类号： C17H9/24 ,  C12Q1/68 ,  C12N15/74 ,  C07H21/04

    CPC分类号： C12N15/52 ,  C07K14/335 ,  Y10T436/143333

    摘要： Fructooligosaccharide (FOS)-related protein nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, FOS-related fusion proteins, antigenic peptides, and anti-FOS-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides of the invention and methods for their use are further disclosed.

    摘要翻译： 低聚果糖（FOS）相关蛋白核酸分子及其多肽及其片段和变体在本发明中公开。 此外，还包括FOS相关融合蛋白，抗原肽和抗FOS相关抗体。 本发明还提供了含有本发明的核酸分子的重组表达载体和已经引入表达载体的宿主细胞。 本发明的多肽的制备方法及其使用方法进一步公开。

    Bacteriophage resistant recombinant bacteria
    5.
    发明授权
    Bacteriophage resistant recombinant bacteria 失效
    耐噬菌细菌重组菌

    公开(公告)号：US5538864A

    公开(公告)日：1996-07-23

    申请号：US373877

    申请日：1995-01-17

    申请人： Colin J. Hill ,  Todd R. Klaenhammer

    发明人： Colin J. Hill ,  Todd R. Klaenhammer

    IPC分类号： A23C9/123 ,  C12N1/04 ,  C12N1/21 ,  C12N15/11 ,  C12N15/34 ,  C12N15/65 ,  C12N15/74 ,  C12N1/20 ,  A23C9/00 ,  C12P7/56

    CPC分类号： C12N15/65 ,  A23C9/1236 ,  C12N1/04 ,  C12N15/11 ,  C12N15/746

    摘要： Recombinant bacteria containing phage-encoded resistance ("Per") and methods of making and using the same are disclosed. Such bacteria are made by (a) conducting a fermentation of a substrate in a medium containing a defined bacterial culture until bacteriophage are detected in the medium, the bacteriophage being specific to at least one bacteria in the medium; (b) isolating the bacteriophage; (c) digesting DNA of the bacteriophage to produce a library of DNA fragments; (d) transforming the bacteria susceptible to said bacteriophage with the library of DNA fragments to provide transformed bacteria; (e) selecting from among the transformed bacteria, a bacteriophage-resistant transformed bacteria; (f) adding bacteriophage resistant transformed bacteria to the medium; and (g) recommencing step (a). Also disclosed are bacterial cells which contain a first bacteriophage defense mechanism (Per), wherein Per comprises a bacteriophage origin of replication (ori) operatively associated with a DNA sequence incapable of producing live bacteriophage. The bacterial cell is capable of being infected by a bacteriophage, the DNA of which, once injected into the bacterial cell, competes with Per for binding to DNA polymerase.

    摘要翻译： 公开了含有噬菌体编码抗性（“Per”）的重组细菌及其制备和使用方法。 这样的细菌通过以下方法制备：（a）在含有限定的细菌培养基的培养基中进行底物的发酵，直到在培养基中检测到噬菌体，噬菌体对培养基中的至少一种细菌是特异性的; （b）分离噬菌体; （c）消化噬菌体的DNA以产生DNA片段文库; （d）用DNA片段的文库转化对所述噬菌体易感的细菌，以提供转化的细菌; （e）从转化细菌中选择抗噬菌体转化细菌; （f）向培养基中加入抗噬菌体转化细菌; 和（g）重新开始步骤（a）。 还公开了含有第一噬菌体防御机制（Per）的细菌细胞，其中Per包含与不能产生活噬菌体的DNA序列可操作地相关的噬菌体复制起点（ori）。 细菌细胞能够被噬菌体感染，其噬菌体一旦被注入到细菌细胞中就与Per结合就与DNA聚合酶结合。

    Genetically modified Streptococcus thermophilus bacterium
    6.
    发明授权
    Genetically modified Streptococcus thermophilus bacterium 有权
    转基因嗜热链球菌

    公开(公告)号：US09040302B2

    公开(公告)日：2015-05-26

    申请号：US13697860

    申请日：2011-05-19

    申请人： Todd R. Klaenhammer ,  Richard L. Guerrant ,  Glynis L. Kolling ,  Evelyn Durmaz ,  Michael P. Timko ,  Cirle Alcantara Warren

    发明人： Todd R. Klaenhammer ,  Richard L. Guerrant ,  Glynis L. Kolling ,  Evelyn Durmaz ,  Michael P. Timko ,  Cirle Alcantara Warren

    IPC分类号： A61K35/74 ,  A61K38/16 ,  A23C9/123 ,  A23L1/30 ,  C12N1/06 ,  C12P21/02 ,  A61K35/00

    CPC分类号： A61K38/164 ,  A23C9/1238 ,  A23L33/135 ,  A61K35/74 ,  A61K2035/11 ,  C12N1/06 ,  C12P21/02

    摘要： Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.

    摘要翻译： 提供用于靶向递送生物治疗剂的方法和组合物。 组合物包含胆汁敏感的嗜热链球菌，其被修饰以在胆汁暴露后释放生物治疗剂。 由本文公开的嗜热链球菌细菌释放的生物治疗剂包括AQ和AQR丰富的肽。 本发明的方法包括向受试者施用在胆汁暴露后经修饰以释放生物治疗剂的嗜热链球菌。 圣氏嗜热链霉菌的施用促进期望的治疗反应。 可以修饰细菌以表达和释放富含AQ或AQR的肽，其随后抑制细胞凋亡或减少粘膜损伤。 因此，本发明的方法用于治疗或预防各种胃肠疾病，包括艰难梭菌感染和抗生素相关性腹泻。

    LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING PROTEASE HOMOLOGUES AND USES THEREFORE
    9.
    发明申请
    LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING PROTEASE HOMOLOGUES AND USES THEREFORE 有权
    Lactobacillus ACIDOPHILUS核酸序列编码蛋白质HOMOLOGE及其用途

    公开(公告)号：US20110020900A1

    公开(公告)日：2011-01-27

    申请号：US12251501

    申请日：2008-10-15

    申请人： Todd R. Klaenhammer ,  Eric Altermann ,  W. Michael Russell

    发明人： Todd R. Klaenhammer ,  Eric Altermann ,  W. Michael Russell

    IPC分类号： C12N9/48 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/50 ,  C07K14/335

    摘要： Protease-like nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, protease-like fusion proteins, antigenic peptides, and anti-protease-like antibodies are encompassed. The invention also provides vectors containing a nucleic acid molecule of the invention and cells into which the vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.

    摘要翻译： 蛋白酶样核酸分子及其多肽及其片段和变体在本发明中公开。 此外，还包括蛋白酶样融合蛋白，抗原肽和抗蛋白酶样抗体。 本发明还提供了含有本发明的核酸分子的载体和已经引入载体的细胞。 进一步公开了制备多肽的方法和本发明多肽的使用方法。