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公开(公告)号：US20090263881A1
公开(公告)日：2009-10-22
申请号：US11953532
申请日：2007-12-10
申请人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
发明人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号： C12N9/26
摘要： The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5时提高的Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。
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公开(公告)号：US07550566B2
公开(公告)日：2009-06-23
申请号：US11463863
申请日：2006-08-10
申请人： Torben Lauesgaard Nissen , Kim Vilbour Andersen , Christian Karsten Hansen , Jan Moller Mikkelsen , Hans Thalsgaard Schambye
发明人： Torben Lauesgaard Nissen , Kim Vilbour Andersen , Christian Karsten Hansen , Jan Moller Mikkelsen , Hans Thalsgaard Schambye
IPC分类号： C07K14/535 , C07K1/107 , A61K38/00 , C07H21/00
摘要： Polypeptide conjugates with G-CSF activity comprising a polypeptide having at least one introduced lysine residue and at least one removed lysine residue compared to the sequence of human G-CSF, and which are conjugated to 2-6 polyethylene glycol moieties. The conjugates have a low in vitro bioactivity, a long in vivo half-life, a reduced receptor-mediated clearance, and provide a more rapid stimulation of production of white blood cells and neutrophils than non-conjugated recombinant human G-CSF.
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公开(公告)号：US07318918B2
公开(公告)日：2008-01-15
申请号：US11132722
申请日：2005-05-18
申请人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Andreas Schaubmar , Hans Koll
发明人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Andreas Schaubmar , Hans Koll
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US06646110B2
公开(公告)日：2003-11-11
申请号：US09760008
申请日：2001-01-10
申请人： Torben Lauesgaard Nissen , Kim Vilbour Andersen , Christian Karsten Hansen , Jan Moller Mikkelsen , Hans Thalsgard Schambye
发明人： Torben Lauesgaard Nissen , Kim Vilbour Andersen , Christian Karsten Hansen , Jan Moller Mikkelsen , Hans Thalsgard Schambye
IPC分类号： C07K100
CPC分类号： A61K9/0019 , A61K38/00 , A61K47/42 , C07K14/535
摘要： The invention relates to polypeptide conjugates comprising a polypeptide exhibiting G-CSF activity and having an amino acid sequence that differs from the amino acid sequence of human G-CSF in at least one specified introduced and/or removed amino acid residue comprising an attachment group for a non-polypeptide moiety, and having at least one non-polypeptide moiety attached to an attachment group of the polypeptide. The attachment group may e.g., be a lysine, cysteine, aspartic acid or glutamic acid residue or a glycosylation site, and the non-polypeptide moiety may e.g., be a polymer such as polyethylene glycol or an oligosaccharide. The conjugate has one or more improved properties such as increased biological half-life and reduced side effects.
摘要翻译： 本发明涉及多肽缀合物，其包含显示G-CSF活性的多肽，并且具有不同于至少一个指定的引入和/或去除的氨基酸残基中的人G-CSF的氨基酸序列的氨基酸序列，所述氨基酸残基包含用于 非多肽部分，并且具有连接到多肽的连接基团的至少一个非多肽部分。 连接基团可以例如是赖氨酸，半胱氨酸，天冬氨酸或谷氨酸残基或糖基化位点，并且非多肽部分可以例如是聚合物，例如聚乙二醇或低聚糖。 缀合物具有一个或多个改进的性质，例如增加的生物半衰期和减少的副作用。
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公开(公告)号：US07531324B2
公开(公告)日：2009-05-12
申请号：US11531557
申请日：2006-09-13
申请人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Andreas Schaubmar , Roberto Falkenstein
发明人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Andreas Schaubmar , Roberto Falkenstein
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US07498152B1
公开(公告)日：2009-03-03
申请号：US11554557
申请日：2006-10-30
CPC分类号： C07K14/56 , A61K9/0019 , A61K38/212 , A61K47/60
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US20090042252A1
公开(公告)日：2009-02-12
申请号：US11554557
申请日：2006-10-30
CPC分类号： C07K14/56 , A61K9/0019 , A61K38/212 , A61K47/60
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US07314613B2
公开(公告)日：2008-01-01
申请号：US10848827
申请日：2004-05-19
CPC分类号： C07K14/56 , A61K9/0019 , A61K38/212 , A61K47/60
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
摘要翻译： 本发明提供了干扰素-α多肽和缀合物，以及编码多肽的核酸。 本发明还包括包含这些多肽，缀合物和核酸的组合物; 含有或表达多肽，缀合物和核酸的细胞; 制备多肽，缀合物和核酸的方法; 以及使用多肽，缀合物和核酸的方法。
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9.发明授权Metabolically engineered microbial cell with an altered metabolite production 有权具有改变的代谢产物的代谢工程的微生物细胞公开(公告)号：US07018829B1
公开(公告)日：2006-03-28
申请号：US09743414
申请日：1999-07-12
CPC分类号： C12N9/0036 , C12N9/0016 , C12N9/93 , C12P7/06 , Y02E50/17
摘要： A recombinant microbial cell comprises at least one increased expressible enzyme activity controlling anabolic metabolism of ammonia as a nutrient source, the increased enzyme activity being an NADH-dependent activity catalysing the reaction (a) 2-oxoglutarate+NH3+NADH→glutamate+NAD or being an activity catalysing the reaction (b) 2-oxoglutarate+glutamine+NADH→2 glutamate+NAD or being an activity catalysing the reaction (c) glutamate+NH3+ATP→glutamine+ADP+Pi. The increased enzyme activity is encoded by a nucleic acid coding sequence linked to an expression signal not natively associated with the nucleic acid coding sequence and is increased as compared to the expression of the enzyme activity when the nucleic acid coding sequence is associated with its native expression signal.
摘要翻译： 重组微生物细胞包含控制氨作为营养源的合成代谢的至少一种增加的可表达酶活性，增加的酶活性是催化反应的NADH依赖性活性（a）2-氧戊二酸盐+ NH 3 > NAD +>谷氨酸+ NAD或作为催化反应的活性（b）2-氧戊二酸+谷氨酰胺+ NADH->谷氨酸+ NAD或催化反应的活性（c）谷氨酸+ NH 3 SUB> + ATP->谷氨酰胺+ ADP + Pi。 增加的酶活性由与核酸编码序列本身不相关的表达信号连接的核酸编码序列编码，并且当核酸编码序列与其天然表达相关时与酶活性的表达相比增加 信号。
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公开(公告)号：US07541163B2
公开(公告)日：2009-06-02
申请号：US11838730
申请日：2007-08-14
申请人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse
发明人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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