公开(公告)号：US4849404A

公开(公告)日：1989-07-18

申请号：US163732

申请日：1988-03-03

申请人： Masakazu Iwai ,  Kazumasa Yokoyama ,  Tsunekazu Fukushima

发明人： Masakazu Iwai ,  Kazumasa Yokoyama ,  Tsunekazu Fukushima

IPC分类号： A61K38/00 ,  C07K16/18

CPC分类号： C07K16/18 ,  A61K38/00 ,  Y10S514/925 ,  Y10S514/926 ,  Y10S514/927 ,  Y10S514/929 ,  Y10S530/866

摘要： Fab or Fc fragment of human IgG which is obtainable by enzymatic digestion of the human IgG is reduced by a reducing agent to sever an interchain of disulfide bonds therein and then blocked the SH radicals formed with alkyl group which may contain other groups. The resulting alkylated Fab and Fc fragments display therapeutic and prophylactic action for gastrointestinal ulcers by the oral or parenteral administration.

公开(公告)号：US4327086A

公开(公告)日：1982-04-27

申请号：US245971

申请日：1981-03-20

申请人： Tsunekazu Fukushima ,  Tomiyuki Matsunaga ,  Satoshi Funakoshi

发明人： Tsunekazu Fukushima ,  Tomiyuki Matsunaga ,  Satoshi Funakoshi

IPC分类号： A61K35/14 ,  A61K35/16 ,  A61K35/50 ,  A61K38/00 ,  A61K38/16 ,  A61L2/00 ,  C12N9/10 ,  A61K37/00 ,  A61K35/48

CPC分类号： C12N9/1044 ,  A61L2/0023 ,  A61K38/00

摘要： A process for heat treatment to inactive hepatitis virus in human blood coagulation factor XIII compositions, which is characterized in that the heat treatment at 50.degree. to 80.degree. C. of an aqueous solution containing the human blood coagulation factor XIII is carried out for 3 to 15 hours in the presence of 10% (W/V) or more of at least one principal stabilizer selected from the group consisting of neutral amino acids, monosaccharides and sugar alcohols and 10% (W/V) or more of at least one auxiliary stabilizer selected from the group consisting of salts of organic carboxylic acids having 3 to 10 carbon atoms.

摘要翻译： 一种在人血液凝固因子XIII组合物中对无活性肝炎病毒进行热处理的方法，其特征在于在含有人血液凝固因子XIII的水溶液中在50〜80℃下进行热处理3次 在10％（W / V）以上的至少一种选自中性氨基酸，单糖和糖醇的主要稳定剂和10％（W / V）以上的至少一种辅助剂 选自具有3-10个碳原子的有机羧酸盐的稳定剂。

公开(公告)号：US4748157A

公开(公告)日：1988-05-31

申请号：US827209

申请日：1986-02-04

申请人： Masakazu Iwai ,  Kazumasa Yokoyama ,  Tsunekazu Fukushima

发明人： Masakazu Iwai ,  Kazumasa Yokoyama ,  Tsunekazu Fukushima

IPC分类号： A61K38/00 ,  C07K16/18 ,  A61K37/00 ,  C07G7/00

CPC分类号： C07K16/18 ,  A61K38/00 ,  Y10S514/925 ,  Y10S514/926 ,  Y10S514/927 ,  Y10S514/929 ,  Y10S530/866

摘要： Fab or Fc fragment of human IgG which is obtainable by enzymatic digestion of the human IgG is reduced by a reducing agent to sever an interchain of disulfide bonds therein and then blocked the SH radicals formed with alkyl group which may contain other group. The resulting alkylated Fab and Fc fragments display therapeutic and prophylactic action for gastrointestinal ulcers by the oral or parenteral administration.

摘要翻译： 通过人IgG的酶消化可获得的人IgG的Fab或Fc片段被还原剂还原，以切断其中二硫键的链间，然后封闭由可能含有其它基团的烷基形成的SH自由基。 所得到的烷基化Fab和Fc片段通过口服或肠胃外给药显示对胃肠溃疡的治疗和预防作用。

公开(公告)号：US4687762A

公开(公告)日：1987-08-18

申请号：US716436

申请日：1985-03-27

申请人： Tsunekazu Fukushima ,  Hiroshi Emoto ,  Yoshio Kagitani ,  Kazumasa Yokoyama ,  Masayuki Nishida ,  Tadakazu Suyama

发明人： Tsunekazu Fukushima ,  Hiroshi Emoto ,  Yoshio Kagitani ,  Kazumasa Yokoyama ,  Masayuki Nishida ,  Tadakazu Suyama

IPC分类号： B01J13/02 ,  A61K9/00 ,  A61K9/127 ,  A61K9/50 ,  A61K31/70 ,  A61K31/545 ,  A61K31/685

CPC分类号： A61K47/48053 ,  A61K9/1277

摘要： The invention provides a method of producing a water-soluble complex comprising a water-insoluble drug and a phospholipid, comprising the steps of (1) dissolving a water-insoluble drug and a phospholipid in an organic solvent, (2) removing the solvent to leave behind a drug-containing phospholipid film, (3) suspending the resulting film in an aqueous solution, (4) ultrasonicating the film, (5) centrifuging the resulting suspension, and (6) recovering the lowermost layer of the resulting sediment to yield the water-soluble complex. The above complex can be administered parenterally as well as by the oral and other routes, and is so rich in the drug component that a remarkably increased drug activity can be realized.

摘要翻译： 本发明提供一种生产包含水不溶性药物和磷脂的水溶性络合物的方法，包括以下步骤：（1）将水不溶性药物和磷脂溶于有机溶剂中，（2）除去溶剂至 留下含药磷脂膜，（3）将所得膜悬浮在水溶液中，（4）使膜超声波化，（5）离心所得悬浮液，（6）回收所得沉淀物的最下层，得到 水溶性复合物。 上述复合物可以肠胃外给药，口服给药或其他途径给药，药物成分丰富，药物活性显着提高。

公开(公告)号：US4624927A

公开(公告)日：1986-11-25

申请号：US600740

申请日：1984-04-16

申请人： Tsunekazu Fukushima ,  Mitsugu Fujii ,  Satoshi Funakoshi ,  Tadakazu Suyama

发明人： Tsunekazu Fukushima ,  Mitsugu Fujii ,  Satoshi Funakoshi ,  Tadakazu Suyama

IPC分类号： G01N33/53 ,  C12Q1/56 ,  G01N33/86 ,  G01N31/00 ,  G01N33/556

CPC分类号： G01N33/86 ,  G01N2333/9108 ,  G01N2333/91085 ,  Y10S436/808 ,  Y10T436/106664

摘要： A reagent for determination of human blood coagulation factor XIII for reversed passive hemagglutination reaction which comprises sensitized erythrocytes prepared by sensitizing animal erythrocytes with specific anti-human factor XIII antibody, and a kit using the reagent are disclosed.

摘要翻译： 公开了一种用于测定用于反向被动血凝反应的人血液凝固因子XIII的试剂，其包括通过用特异性抗人因子XIII抗体对动物红细胞进行敏化制备的致敏红细胞和使用该试剂的试剂盒。