公开(公告)号：US12173282B2

公开(公告)日：2024-12-24

申请号：US17030232

申请日：2020-09-23

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Pankaj Garg ,  Qiang Liu ,  Tom Yuan

IPC: C12N15/10 ,  C07K16/28

Abstract: Provided herein are methods and compositions relating to variant nucleic acid libraries encoding for antibodies including single domain antibodies. Libraries generated using methods described herein have improved characteristics including improved binding affinity. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US20240400710A1

公开(公告)日：2024-12-05

申请号：US18664201

申请日：2024-05-14

Applicant: Twist Bioscience Corporation

Inventor： Aaron SATO ,  Qiang LIU ,  Tom YUAN

IPC: C07K16/28 ,  A61K39/00 ,  C07K16/00 ,  C07K16/10

Abstract: Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding SARS-CoV-2 or ACE2 antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US20240344057A1

公开(公告)日：2024-10-17

申请号：US18647281

申请日：2024-04-26

Applicant: Twist Bioscience Corporation

Inventor： Jeremy LACKEY ,  David DODD

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US12056264B2

公开(公告)日：2024-08-06

申请号：US17938228

申请日：2022-10-05

Applicant: Twist Bioscience Corporation

Inventor： Bill James Peck

IPC: G06F21/78 ,  C12N15/10

CPC classification number: G06F21/78 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/1089 ,  G06F2212/1052 ,  G06F2212/402

Abstract: Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.

公开(公告)号：US12018065B2

公开(公告)日：2024-06-25

申请号：US17242170

申请日：2021-04-27

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Qiang Liu ,  Tom Yuan

IPC: C07K16/10 ,  C07K16/00 ,  A61K39/00

CPC classification number: C07K16/10 ,  C07K16/005 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92

Abstract: Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding SARS-CoV-2 or ACE2 antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US11970697B2

公开(公告)日：2024-04-30

申请号：US17504358

申请日：2021-10-18

Applicant: Twist Bioscience Corporation

Inventor： Jeremy Lackey ,  David Dodd

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US20240001325A1

公开(公告)日：2024-01-04

申请号：US18345869

申请日：2023-06-30

Applicant: Twist Bioscience Corporation

Inventor： Eugene P. MARSH ,  Pierre F. INDERMUHLE ,  Bill James PECK

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00722 ,  B01J2219/00596 ,  B01J2219/00533 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00662 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00509 ,  B01J2219/00716 ,  B01J2219/00659

Abstract: Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.

公开(公告)号：US11745159B2

公开(公告)日：2023-09-05

申请号：US17122988

申请日：2020-12-15

Applicant: Twist Bioscience Corporation

Inventor： Eugene P. Marsh ,  Pierre F. Indermuhle ,  Bill James Peck

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00509 ,  B01J2219/00533 ,  B01J2219/00596 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00716 ,  B01J2219/00722

Abstract: Defined sequence RNA synthesis by 3′→5′ direction is now well established and currently in use for synthesis and development of vast variety of therapeutic grade RNA and Si RNA etc. A number of such synthetic RNA requires a modification or labeling of 3′-end of an oligonucleotide. The synthesis of 3′-end modified RNA requiring lipophilic, long chain ligands or chromophores, using 3′→5′ synthesis methodology is challenging, requires corresponding solid support and generally results in low coupling efficiency and lower purity of the final oligonucleotide in general because of large amount of truncated sequences containing desired hydrophobic modification. We have approached this problem by developing reverse RNA monomer phosphoramidites for RNA synthesis in 5′→3′-direction. They lead to very clean oligonucleotide synthesis allowing for introduction of various modifications at the 3′-end.

公开(公告)号：US20230257448A1

公开(公告)日：2023-08-17

申请号：US18149593

申请日：2023-01-03

Applicant: Twist Bioscience Corporation

Inventor： Aaron SATO

IPC: C07K16/10 ,  A61P31/14

CPC classification number: C07K16/10 ,  A61P31/14 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/35 ,  C07K2317/92 ,  A61K2039/545

Abstract: Provided herein are methods and compositions relating to improved bispecific antibodies capable of binding and neutralizing SARS-CoV-2 variants.

公开(公告)号：US20230193513A1

公开(公告)日：2023-06-22

申请号：US17937377

申请日：2022-09-30

Applicant: Twist Bioscience Corporation

Inventor： Ray TABIBIAZAR ,  Aaron SATO ,  Pankaj GARG ,  Qiang LIU ,  Fumiko AXELROD

IPC: C40B40/08 ,  C07K16/28

CPC classification number: C40B40/08 ,  C07K16/2869 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2317/92

Abstract: Provided herein are methods and compositions relating to glucagon-like peptide-1 receptor (GLP1R) libraries having nucleic acids encoding for a scaffold comprising a GLP1R binding domain. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.