-
公开(公告)号：US12188024B2
公开(公告)日：2025-01-07
申请号：US18316222
申请日：2023-05-11
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
-
公开(公告)号：US20210108215A1
公开(公告)日：2021-04-15
申请号：US17047757
申请日：2018-06-25
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
-
公开(公告)号：US20250027096A1
公开(公告)日：2025-01-23
申请号：US18904008
申请日：2024-10-01
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
Abstract: The present disclosure provides a transformant obtained by introducing an aldehyde-alcohol dehydrogenase gene into a bacterium of the genus Hydrogenophilus.
-
公开(公告)号：US11697817B2
公开(公告)日：2023-07-11
申请号：US17047757
申请日：2018-06-25
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
CPC classification number: C12N15/74 , C12P7/06 , C12P7/16 , C12Y101/01001 , C12Y401/01001
Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source.
(a1) DNA which consists of a base sequence of SEQ ID NO: 1;
(a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity;
(a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
-
公开(公告)号：US20220290109A1
公开(公告)日：2022-09-15
申请号：US17631066
申请日：2019-08-09
Inventor： Hideaki Yukawa , Naoto Ohtani
Abstract: When lactate dehydrogenase gene and/or malate/lactate dehydrogenase gene is/are introduced into a Hydrogenophilus bacterium as well as one or more of the three lactic acid-utilizing enzyme genes on the genome of the Hydrogenophilus bacterium is/are disrupted, lactic acid-producing ability is remarkably increased. The inventors of the present invention have identified the three lactic acid-utilizing enzyme genes of the Hydrogenophilus bacterium. When lactate permease gene is further introduced into the recombinant, lactic acid-producing ability is further increased. The recombinant of the present invention effectively produces lactic acid using carbon dioxide as a sole carbon source, and therefore, it is able to efficiently produce the material of biodegradable plastics, while solving global warming caused by increased emissions of carbon dioxide.
-
公开(公告)号：US20210403928A1
公开(公告)日：2021-12-30
申请号：US17292867
申请日：2018-11-30
Inventor： Yukawa Hideaki , Naoto Ohtani
Abstract: A transformant obtained by introducing (a) a lactate dehydrogenase gene and/or (b) a malate/lactate dehydrogenase gene into a Hydrogenophilus bacterium efficiently produces lactic acid through use of carbon dioxide as a sole carbon source. Parageobacillus thermoglucosidasius ldh gene, Geobacillus kaustophilus ldh gene and Thermus thermophilus ldh gene of lactate dehydrogenases, and Thermus thermophilus mldh gene and Meiothermus ruber mldh-1 and mldh-2 genes of malate/lactate dehydrogenases are preferable in that they have good lactic acid production efficiency.
-
公开(公告)号：US20230340504A1
公开(公告)日：2023-10-26
申请号：US18316224
申请日：2023-05-11
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
CPC classification number: C12N15/74 , C12P7/06 , C12P7/16 , C12Y101/01001 , C12Y401/01001
Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source.
(a1) DNA which consists of a base sequence of SEQ ID NO: 1;
(a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity;
(a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
-
公开(公告)号：US20210403927A1
公开(公告)日：2021-12-30
申请号：US17292870
申请日：2018-12-12
Inventor： Hideaki Yukawa , Naoto Ohtani
Abstract: Hydrogenophilus bacterium which produces a mutant acetolactate synthase III small subunit formed of a mutant amino acid sequence having an amino acid substitution is able to effectively produce valine through use of carbon dioxide as a sole carbon source.
-
公开(公告)号：US12188025B2
公开(公告)日：2025-01-07
申请号：US18316224
申请日：2023-05-11
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
-
公开(公告)号：US20230340503A1
公开(公告)日：2023-10-26
申请号：US18316222
申请日：2023-05-11
Inventor： Hideaki Yukawa , Naoto Ohtani , Masaharu Ishii
CPC classification number: C12N15/74 , C12P7/06 , C12P7/16 , C12Y101/01001 , C12Y401/01001
Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source.
(a1) DNA which consists of a base sequence of SEQ ID NO: 1;
(a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity;
(a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
