公开(公告)号：US11860076B2

公开(公告)日：2024-01-02

申请号：US16934045

申请日：2020-07-21

申请人： Vilnius University ,  Droplet Genomics

发明人： Linas Mazutis ,  Greta Stonyte ,  Karolis Leonavicius ,  Ausra Zelvyte

IPC分类号： G01N1/40 ,  C12Q1/68 ,  B01J13/04 ,  B01J13/20 ,  C12Q1/6806

CPC分类号： G01N1/4077 ,  B01J13/046 ,  B01J13/206 ,  C12Q1/6806

摘要： The present invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system and then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures in order to release the encapsulated species. The present invention exemplifies the isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis. Finally, the present invention also exemplifies the use of capsules in multi-step procedures to perform complex biological reactions.

公开(公告)号：US20230151409A1

公开(公告)日：2023-05-18

申请号：US17916056

申请日：2020-03-30

申请人： Vilnius University

发明人： Edita Kriukiene ,  Juozas Gordevicius ,  Milda Narmonte ,  Povilas Gibas

IPC分类号： C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/686

CPC分类号： C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q2600/154 ,  C12Q2600/118

摘要： This invention relates to a method that covalently modifies unmodified and hydroxymethylated genomic sites in fetal specific genetic material present in maternal blood DNA samples and produce the adjacent genomic regions for detecting fetal aneuploidies and fetal gender using quantitative real time PCR or sequencing. A large panel of differently labeled sites and regions between maternal and fetal genetic material has been identified and they validity for diagnostic purposes of fetal trisomy of chromosome 21 has been demonstrated.

公开(公告)号：US20210147909A1

公开(公告)日：2021-05-20

申请号：US17054637

申请日：2019-05-10

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  VILNIUS UNIVERSITY

发明人： STEPHANE DESCHAMPS ,  VIRGINIJUS SIKSNYS ,  JOSHUA K YOUNG ,  MINDAUGAS ZAREMBA

IPC分类号： C12Q1/6806 ,  C12Q1/6869

摘要： Methods and compositions are provided for the identification, detection, characterization, and/or utilization of double strand breaks in a target polynucleotide; the identification, detection, characterization, and/or utilization of cutting sites for double-strand-break-inducing agents; and the identification, detection, characterization, and/or utilization of double-strand-break-inducing agents.

公开(公告)号：US20210130799A1

公开(公告)日：2021-05-06

申请号：US16618238

申请日：2018-05-31

申请人： VILNIUS UNIVERSITY

发明人： Virginijus SIKSNYS ,  Migle KAZLAUSKIENE ,  Georgij KOSTIUK ,  Gintautas TAMULAITIS

IPC分类号： C12N9/22 ,  C12N9/12 ,  C12P19/34

摘要： A method of synthetizing cyclic oligoadenylates using a novel catalytic activity of a protein possessing the Palm domain, such as the Cas10 protein, and using such compounds for activation of proteins possessing the CARF doman, such as the Csm6 protein.

公开(公告)号：US20190002943A1

公开(公告)日：2019-01-03

申请号：US16069404

申请日：2017-01-10

申请人： VILNIUS UNIVERSITY ,  ETH ZURICH

发明人： Linas MAZUTIS ,  Greta STONYTE ,  Vaidotas KISELIOVAS ,  Rapolas ZILIONIS ,  Arvydas JANULAITIS ,  Robertas GALINIS ,  Sabine STUDER ,  Donald HILVERT

IPC分类号： C12P19/34 ,  B01L3/00

摘要： Disclosed is a system and method for production of DNA particles and use thereof. The DNA particles can be produced by amplification of nucleic acid molecule(s). Alternatively, DNA particles can be prepared by condensing multiple DNA molecules. The DNA condensation into a particle is mainly triggered by pyrophosphate and positively charged cations (e.g. magnesium). DNA particles can be applied for numerous biological applications but not limited to directed evolution, proteomics, drug delivery and imaging. DNA particles can be used to synthesize proteins using in vitro transcription/translation reaction.

公开(公告)号：US09725467B2

公开(公告)日：2017-08-08

申请号：US14435649

申请日：2012-10-30

申请人： VILNIUS UNIVERSITY

发明人： Daumantas Matulis ,  Virginija Dudutiene ,  Asta Zubriene

IPC分类号： C07D513/04 ,  C07D233/84 ,  C07C311/29 ,  C07C311/39 ,  C07C317/14 ,  C07C317/18 ,  C07C317/36 ,  C07D239/38 ,  C07C323/67 ,  C07D277/74 ,  C07D295/096 ,  C07C311/37 ,  C07C317/22 ,  C07D237/18

CPC分类号： C07D513/04 ,  C07C311/29 ,  C07C311/37 ,  C07C311/39 ,  C07C317/14 ,  C07C317/18 ,  C07C317/22 ,  C07C317/36 ,  C07C323/67 ,  C07C2601/14 ,  C07C2601/18 ,  C07C2601/20 ,  C07C2602/08 ,  C07C2602/10 ,  C07C2603/74 ,  C07D233/84 ,  C07D237/18 ,  C07D239/38 ,  C07D277/74 ,  C07D295/096

摘要： Novel fluorinated benzenesulfonamides compounds of general formula (I) can be used in biomedicine as active ingredients in pharmaceutical formulations, because they inhibit enzymes which participate in disease progression.

公开(公告)号：US20170198286A1

公开(公告)日：2017-07-13

申请号：US15448818

申请日：2017-03-03

申请人： VILNIUS UNIVERSITY

发明人： Virginijus Siksnys ,  Migle Kazlauskiene ,  Gintautas Tamulaitis

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/113

CPC分类号： C12N15/111 ,  C07K14/315 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/10 ,  C12N2310/20 ,  C12Y301/27

摘要： A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.

公开(公告)号：US20170016055A1

公开(公告)日：2017-01-19

申请号：US15072677

申请日：2016-03-17

申请人： Vilnius University

发明人： Saulius Klimasauskas ,  Zdislav Stasevskij ,  Edita Kriukiene

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12P19/34 ,  C12Q1/6846 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2521/125 ,  C12Q2525/117 ,  C12Q2525/155 ,  C12Q2537/1373

摘要： A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer based on the template nucleic acid sequence, wherein the linking unit is attached to a target site in the template nucleic acid sequence with a covalent linkage.

摘要翻译： 一种从模板核酸序列产生核酸分子的方法和连接于引物的连接单元，该方法包括在使核酸聚合酶产生的条件下使模板核酸序列与核酸聚合酶接触的步骤 基于模板核酸序列的来自引物的核酸分子，其中所述连接单元通过共价键连接到模板核酸序列中的靶位点。

公开(公告)号：US20150259725A1

公开(公告)日：2015-09-17

申请号：US14407978

申请日：2013-05-30

申请人： VILNIUS UNIVERSITY

发明人： Saulius Klimasauskas ,  Giedrius Vilkaitis ,  Aleksandra Plotnikova

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2320/51

摘要： A method for modifying a strand of RNA at the 3′ end, includes contacting the strand with a RNA 2′-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the RNA 2′-O-methyltransferase of a part of the co-factor onto the 3′ end of the RNA strand to form a modified RNA strand, wherein the strand of RNA is included in a duplex, and wherein the part of the co-factor transferred includes a reporter group or a functional group.

摘要翻译： 在3'末端修饰RNA链的方法包括在辅助因子存在下，将该链与RNA 2'-O-甲基转移酶接触，在允许通过RNA 2'-O 一部分辅因子的α-甲基转移酶转移到RNA链的3'末端以形成修饰的RNA链，其中所述RNA链包含在双链体中，并且其中转运的辅因子的部分包括记者 组或功能组。

公开(公告)号：US20150093752A1

公开(公告)日：2015-04-02

申请号：US14542770

申请日：2014-11-17

申请人： Vilnius University

发明人： Saulius Klimasauskas ,  Zita Liutkeviciute ,  Edita Kriukiene

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C07H21/00 ,  C12Q1/48 ,  C12Q1/6816 ,  G01N2333/91011 ,  C12Q2563/107 ,  C12Q2521/125

摘要： The present invention relates to targeted conversion of alpha-hydroxyalkylated residues in biomolecules in the presence of a directing methyltransferase, namely to targeted removal of the alpha-hydroxyalkyl moieties to give unmodified residues, or targeted derivatization of the alpha-hydroxyalkyl groups by covalent coupling of non-cofactor compounds represented by formula HQ-LX, wherein X represents a functional group or a reporter group attached via a linker moiety L, and QH is selected from HS—, HSe—, HO—H2N—, HN3 or HCN in the presence of a directing methyltransferase. Further development of the method of targeted conversion comprises methods for targeted labeling a biomolecule and method for detecting hydroxymethylated target sites in a biomolecule according to the present invention.

摘要翻译： 本发明涉及在导向甲基转移酶存在下生物分子中的α-羟烷基化残基的靶向转化，即靶向除去α-羟烷基部分以得到未修饰的残基，或通过共价偶联的α-羟基烷基的靶向衍生化 由式HQ-LX表示的非辅助因子化合物，其中X表示通过连接体部分L连接的官能团或报告基团，QH选自HS-，HSe-，HO-H2N-，HN3或HCN， 的甲基转移酶。 靶向转化方法的进一步开发包括用于靶向生物分子标记的方法和用于检测根据本发明的生物分子中的羟甲基化靶位点的方法。