公开(公告)号：US06326204B1

公开(公告)日：2001-12-04

申请号：US09116188

申请日：1998-07-15

申请人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Steve Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Steve Bass

IPC分类号： C12N1500

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：US06251674B1

公开(公告)日：2001-06-26

申请号：US09499505

申请日：2000-02-07

申请人： Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull

发明人： Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull

IPC分类号： C12N1500

CPC分类号： A01H1/00 ,  A01K2217/05 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12Q1/6811

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06716631B1

公开(公告)日：2004-04-06

申请号：US09516695

申请日：2000-03-01

申请人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten

发明人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten

IPC分类号： C12N1500

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：US06528311B1

公开(公告)日：2003-03-04

申请号：US09516051

申请日：2000-03-01

申请人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

IPC分类号： C12N1500

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06399383B1

公开(公告)日：2002-06-04

申请号：US09181072

申请日：1998-10-27

申请人： Doris Apt ,  Paul Khavari ,  William P. C Stemmer

发明人： Doris Apt ,  Paul Khavari ,  William P. C Stemmer

IPC分类号： C12N1585

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/20043 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/60

摘要： The invention provides human papillomavirus vectors useful in gene therapy. Such a vector contains E1 and E2 coding regions from a benign or low-risk human papillomavirus operably linked to a promoter and enhancer, and an LCR region from a human papillomavirus comprising an origin of replication including binding sites for the E1 and E2 proteins. The invention further provides methods of using such vectors in gene therapy, methods of controlling expression using a patch, and methods of using such vectors to evolve drugs for stimulation of hair growth or alteration of hair color.

摘要翻译： 本发明提供可用于基因治疗的人乳头状瘤病毒载体。 这样的载体含有可操作地连接于启动子和增强子的良性或低危人乳头瘤病毒的E1和E2编码区，以及包含复制起点的人乳头瘤病毒的LCR区，包括E1和E2蛋白的结合位点。 本发明还提供了在基因治疗中使用这种载体的方法，使用贴剂控制表达的方法，以及使用这些载体来产生用于刺激头发生长或改变头发颜色的药物的方法。

公开(公告)号：US06352859B1

公开(公告)日：2002-03-05

申请号：US09626343

申请日：2000-07-26

申请人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

IPC分类号： C12N1500

CPC分类号： A01H1/00 ,  A01K2217/05 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12Q1/6811

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.