公开(公告)号：US20120005773A1

公开(公告)日：2012-01-05

申请号：US12998243

申请日：2009-09-30

申请人： Eric D. Aasen ,  Mark Scott Abad ,  Edwards Allen ,  Thandoni Rao Ambika ,  Veena S. Anil ,  Alice Clara Augustine ,  Stephanie L. Back ,  Pranesh Badami ,  Amarjit Basra ,  Kraig L. Brustad ,  Molian Deng ,  Todd Dezwaan ,  Charles Dietrich ,  Stephen M. Duff ,  Karen K. Gabbert ,  Steve He ,  Bill L. Hendrix ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Balasulojini Karunanandaa ,  Kevin R. Kosola ,  Kempagangaiah Krishnamurthy ,  Ganesh Kumar ,  Saritha V. Kuriakose ,  Shantala Lakkanna ,  Mark E. Leibman ,  Linda Lutfiyya ,  Savitha Madappa ,  Sivalinganna Manjunath ,  Robert J. Meister ,  Bellur Narasimba Prasad ,  Sasha Preuss ,  Qungang Qi ,  Dhanalakshmi Ramachandra ,  S. Sangeetha ,  Scott Saracco ,  Chitresh Sharma ,  Vijay K. Sharma ,  Rajani Monnanda Somaiah ,  Padmini Sudarshana ,  Rebecca L. Thompson-Mize ,  Srikanth Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Jingrui Wu ,  Xiaoyun Wu ,  Xiaofeng Sean Yang ,  Qin Zeng ,  Jianmin Zhao

发明人： Eric D. Aasen ,  Mark Scott Abad ,  Edwards Allen ,  Thandoni Rao Ambika ,  Veena S. Anil ,  Alice Clara Augustine ,  Stephanie L. Back ,  Pranesh Badami ,  Amarjit Basra ,  Kraig L. Brustad ,  Molian Deng ,  Todd Dezwaan ,  Charles Dietrich ,  Stephen M. Duff ,  Karen K. Gabbert ,  Steve He ,  Bill L. Hendrix ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Balasulojini Karunanandaa ,  Kevin R. Kosola ,  Kempagangaiah Krishnamurthy ,  Ganesh Kumar ,  Saritha V. Kuriakose ,  Shantala Lakkanna ,  Mark E. Leibman ,  Linda Lutfiyya ,  Savitha Madappa ,  Sivalinganna Manjunath ,  Robert J. Meister ,  Bellur Narasimba Prasad ,  Sasha Preuss ,  Qungang Qi ,  Dhanalakshmi Ramachandra ,  S. Sangeetha ,  Scott Saracco ,  Chitresh Sharma ,  Vijay K. Sharma ,  Rajani Monnanda Somaiah ,  Padmini Sudarshana ,  Rebecca L. Thompson-Mize ,  Srikanth Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Jingrui Wu ,  Xiaoyun Wu ,  Xiaofeng Sean Yang ,  Qin Zeng ,  Jianmin Zhao

IPC分类号： A01H1/02 ,  C12N15/63 ,  A01G7/00 ,  A01H5/00 ,  A01H5/10 ,  A23L1/20 ,  C12N15/29 ,  C12N5/10

CPC分类号： C12N15/8251 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐冷性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20090075287A1

公开(公告)日：2009-03-19

申请号：US12208629

申请日：2008-09-11

申请人： Andre R. Abad ,  Ronald D. Flannagan ,  Bin Hu ,  Billy F. McCutchen ,  Xiaofeng Sean Yang ,  Cao Guo Yu

发明人： Andre R. Abad ,  Ronald D. Flannagan ,  Bin Hu ,  Billy F. McCutchen ,  Xiaofeng Sean Yang ,  Cao Guo Yu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q1/689 ,  C12Q2541/101

摘要： Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.

摘要翻译： 提供了鉴定新型杀虫基因同系物的方法和组合物。 具体地说，本发明的方法包括系统地设计针对目的杀虫基因特异性的寡核苷酸引物，并对来自微生物特别是苏云金芽孢杆菌属菌株的核酸材料进行连续循环的PCR扩增，以鉴定已知杀虫基因的新型同系物 。 进一步公开了可用于实施本方法的寡核苷酸引物。

公开(公告)号：US07452700B2

公开(公告)日：2008-11-18

申请号：US11404741

申请日：2006-04-14

申请人： Andre R. Abad ,  Ronald D. Flannagan ,  Bin Hu ,  Billy F. McCutchen ,  Xiaofeng Sean Yang ,  Cao Guo Yu

发明人： Andre R. Abad ,  Ronald D. Flannagan ,  Bin Hu ,  Billy F. McCutchen ,  Xiaofeng Sean Yang ,  Cao Guo Yu

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6809 ,  C12Q1/689 ,  C12Q2541/101

摘要： Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.

摘要翻译： 提供了鉴定新型杀虫基因同系物的方法和组合物。 具体地说，本发明的方法包括系统地设计针对目的杀虫基因特异性的寡核苷酸引物，并对来自微生物特别是苏云金芽孢杆菌属菌株的核酸材料进行连续循环的PCR扩增，以鉴定已知杀虫基因的新型同系物 。 进一步公开了可用于实施本发明方法的寡核苷酸引物。