Methods and applications for stitched DNA barcodes
    3.
    发明授权
    Methods and applications for stitched DNA barcodes 有权
    缝合DNA条形码的方法和应用

    公开(公告)号:US08268564B2

    公开(公告)日:2012-09-18

    申请号:US12236912

    申请日:2008-09-24

    IPC分类号: C12P19/34

    摘要: Methods of identifying the genotypes of a plurality of single cells, wherein each cell includes a plurality of DNA barcodes, each associated with a genetic mutation or marker, are provided. In particular, methods including linking a plurality of DNA barcodes together to create a stitched barcode, amplifying the stitched barcode and sequencing the stitched barcode are provided. Also provided are methods of determining the presence of at least one genetic mutation in a population of cells.

    摘要翻译: 鉴定多个单细胞的基因型的方法,其中每个细胞包括多个DNA条形码,每一个都与基因突变或标记相关联。 特别地,提供了将多个DNA条形码连接在一起以创建缝合条形码,放大缝合条形码并对拼接条形码排序的方法。 还提供了确定细胞群中至少一种遗传突变的存在的方法。

    METHODS AND APPLICATIONS FOR STITCHED DNA BARCODES
    5.
    发明申请
    METHODS AND APPLICATIONS FOR STITCHED DNA BARCODES 有权
    用于染色DNA条带的方法和应用

    公开(公告)号:US20090098555A1

    公开(公告)日:2009-04-16

    申请号:US12236912

    申请日:2008-09-24

    IPC分类号: C12Q1/68 C40B50/06

    摘要: Methods of identifying the genotypes of a plurality of single cells, wherein each cell includes a plurality of DNA barcodes, each associated with a genetic mutation or marker, are provided. In particular, methods including linking a plurality of DNA barcodes together to create a stitched barcode, amplifying the stitched barcode and sequencing the stitched barcode are provided. Also provided are methods of determining the presence of at least one genetic mutation in a population of cells.

    摘要翻译: 鉴定多个单细胞的基因型的方法,其中每个细胞包括多个DNA条形码,每一个都与基因突变或标记相关联。 特别地,提供了将多个DNA条形码连接在一起以创建缝合条形码,放大缝合条形码并对拼接条形码排序的方法。 还提供了确定细胞群中至少一种遗传突变的存在的方法。

    Substrate processing method and substrate processing apparatus
    7.
    发明授权
    Substrate processing method and substrate processing apparatus 失效
    基板处理方法和基板处理装置

    公开(公告)号:US06485203B2

    公开(公告)日:2002-11-26

    申请号:US09737473

    申请日:2000-12-18

    IPC分类号: G03D500

    摘要: A developing unit, a coating unit and a plurality of cooling plates are arranged in a process station which performs a resist coating and so on and a wafer is transferred among them by a substrate transfer device. The temperature of an area to where the wafer is transferred is detected by a temperature/humidity detector and the temperature of the wafer which is cooled by the cooling plates is adjusted accordingly based on a detected value so that the temperature of the wafer when transferred to the coating unit becomes a coating temperature of a processing solution. Thereby, the wafer is transferred to the coating unit while maintaining its temperature with high accuracy to be coated with a resist solution, so that a formation of an uneven processing due to the temperature change can be prevented and a uniform processing can be performed.

    摘要翻译: 显影单元,涂布单元和多个冷却板布置在执行抗蚀剂涂层等的处理站中,并且通过基板传送装置在晶片之间转印晶片。 通过温度/湿度检测器检测晶片转移到的区域的温度,并且基于检测值相应地调节由冷却板冷却的晶片的温度,使得晶片转移到 涂布单元成为处理溶液的涂布温度。 由此,将晶片转印到涂布单元,同时高精度地保持其温度以涂覆抗蚀剂溶液,从而可以防止由温度变化引起的不均匀处理的形成,并且可以进行均匀的处理。