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公开(公告)号：US08542274B2
公开(公告)日：2013-09-24
申请号：US12906411
申请日：2010-10-18
申请人： Yoshihiro Kawano , Lorne D. Davies
发明人： Yoshihiro Kawano , Lorne D. Davies
IPC分类号： H04N13/00
CPC分类号： G02B21/365
摘要： A wide field microscopic image acquisition apparatus and method are disclosed. The apparatus is configured to acquire images of a specimen on a microscope slide and includes first and second illuminators each having unique illumination characteristics. The apparatus includes a microscope imaging system with an imaging device, an objective lens and a stage configured to digitally acquire a plurality of images of the specimen using the first and second illuminators. A controller is configured to automatically control the microscope imaging system and acquire the plurality of images of the specimen using the first and second illuminators. The first and second illuminators can be bright field, dark field or fluorescent illuminators.
摘要翻译： 公开了一种宽视野显微镜图像采集装置和方法。 该装置被配置为在显微镜载玻片上获取样本的图像，并且包括具有独特的照明特性的第一和第二照明器。 该装置包括具有成像装置，物镜和配置为使用第一和第二照明器数字获取样本的多个图像的台的显微镜成像系统。 控制器被配置为自动控制显微镜成像系统并且使用第一和第二照明器获取样本的多个图像。 第一和第二个照明器可以是明场，暗场或荧光灯。
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公开(公告)号：US08350904B2
公开(公告)日：2013-01-08
申请号：US12578912
申请日：2009-10-14
申请人： Yasushi Fujimoto , Yoshihiro Kawano
发明人： Yasushi Fujimoto , Yoshihiro Kawano
CPC分类号： G02B21/02 , G02B21/086 , G02B21/367
摘要： A microscope for virtual-slide creating system has a stage for holding the specimen, a transmitted-light illumination optical system for illuminating the specimen with transmitted light, an objective, a tube lens and an image capture unit. The objective is configured as a dry system of infinity-corrected type with an object-side numerical aperture of 0.8 or greater and a focal length for d-line rays in a range from 8 to 20 mm. The tube lens has a focal length in a range from 160 to 280 mm. The image capture surface of the image capture unit has a long side of 12 mm or longer and a pixel size (μm) satisfying the following condition: a (μm)≦(0.61×0.59 (μ/m))/NA′ where a is the pixel size, and NA′ is an image-side numerical aperture.
摘要翻译： 用于虚拟幻灯片创建系统的显微镜具有用于保持样本的阶段，用于用透射光照射样本的透射照明光学系统，物镜，管透镜和图像捕获单元。 该目的被配置为具有0.8或更大的物体侧数值孔径和8至20mm范围内的d线光线的焦距的无限校正型的干式系统。 管透镜的焦距在160至280mm的范围内。 图像捕获单元的图像捕获表面具有12mm或更长的长边和满足以下条件的像素大小（μm）：a（μm）＆nlE;（0.61×0.59（μ/ m））/ NA'其中 a是像素尺寸，NA'是图像侧数值孔径。
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公开(公告)号：US08186305B2
公开(公告)日：2012-05-29
申请号：US12308797
申请日：2007-06-27
申请人： Yoshihiro Kawano
发明人： Yoshihiro Kawano
IPC分类号： A01K29/00
CPC分类号： A61D7/04 , A61M16/01 , A61M16/0627 , A61M16/18 , A61M2250/00
摘要： The present invention prevents the leakage of anesthetic gas, and enables in vivo observation of a specimen such as small laboratory animals over a long period of time. An in-vivo examination apparatus (1) comprises: a stage (2) for mounting a specimen (A) such as a laboratory animal; an anesthetizing chamber (3) which is disposed on the stage (2) for housing the specimen (A); and an anesthetic gas supply device (4) for supplying anesthetic gas (G) into the anesthetizing chamber (3); wherein a transparent window (3c) is provided on at least a part of the anesthetizing chamber (3).
摘要翻译： 本发明防止了麻醉气体的泄漏，并且能够长时间地在小型实验动物体内观察样本。 体内检查装置（1）包括：用于安装诸如实验室动物的样本（A）的台（2） 麻醉室（3），其设置在所述载物台（2）上用于容纳所述试样（A）; 以及用于向麻醉室（3）供给麻醉气体（G）的麻醉气体供给装置（4）。 其中在所述麻醉室（3）的至少一部分上设置透明窗口（3c）。
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公开(公告)号：US07801589B2
公开(公告)日：2010-09-21
申请号：US11640921
申请日：2006-12-19
IPC分类号： A61B6/00
CPC分类号： A61B1/045 , A61B1/043 , A61B5/0068 , A61B5/0071 , A61B5/0084
摘要： An object is to easily and reliably reach an examination site. The present invention provides an examination method of carrying out in-vivo examination using an in-vivo examination apparatus capable of fluorescence examination and reflected-light examination and includes the steps of setting a reference position so as to set an examination site by carrying out reflected-light examination, switching from reflected-light examination to fluorescence examination, and moving from the reference position to the examination site.
摘要翻译： 目的是轻松可靠地到达考场。 本发明提供使用能够进行荧光检查和反射光检查的体内检查装置进行体内检查的检查方法，包括以下步骤：设定参考位置，以便通过进行反射 光线检查，从反射光检查切换到荧光检查，从参考位置移动到检查点。
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公开(公告)号：US07609440B2
公开(公告)日：2009-10-27
申请号：US11667338
申请日：2005-11-09
CPC分类号： G02B21/0028 , G02B21/082 , G02B21/361 , G02B21/362
摘要： When carrying out in vivo examination of a living organism, the behavior in the interior thereof is continuously observed with clear images. Provided is an in-vivo examination apparatus 1 comprising an optical unit 4 including a light source 2 and an image acquisition device 3; and an observation head 5, which is disposed with a gap with respect to the optical unit 4 and which is fixed to a living organism A, wherein the optical unit 4 includes a first collimator optical system 7 for converting light from the light source 2 into collimated light L1 and introducing it into the observation head 5 and an image-forming optical system 9 for focusing return light from the observation head 5 to form an image at the image-acquisition device 3, and wherein the observation optical head 5 includes a second collimator optical system 15 for focusing the collimated light L1 incident from the optical unit 4 to irradiate an examination target site B in the living organism A, as well as for converting return light from the living organism into collimated light L2 and introducing it into the optical unit 4.
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公开(公告)号：US20090067042A1
公开(公告)日：2009-03-12
申请号：US11667338
申请日：2005-11-09
IPC分类号： G02B21/36
CPC分类号： G02B21/0028 , G02B21/082 , G02B21/361 , G02B21/362
摘要： When carrying out in vivo examination of a living organism, the behavior in the interior thereof is continuously observed with clear images. Provided is an in-vivo examination apparatus 1 comprising an optical unit 4 including a light source 2 and an image acquisition device 3; and an observation head 5, which is disposed with a gap with respect to the optical unit 4 and which is fixed to a living organism A, wherein the optical unit 4 includes a first collimator optical system 7 for converting light from the light source 2 into collimated light L1 and introducing it into the observation head 5 and an image-forming optical system 9 for focusing return light from the observation head 5 to form an image at the image-acquisition device 3, and wherein the observation optical head 5 includes a second collimator optical system 15 for focusing the collimated light L1 incident from the optical unit 4 to irradiate an examination target site B in the living organism A, as well as for converting return light from the living organism into collimated light L2 and introducing it into the optical unit 4.
摘要翻译： 在进行生物体的体内检查时，以清晰的图像连续观察其内部的行为。 提供一种体内检查装置1，其包括：包含光源2和图像获取装置3的光学单元4; 以及观察头5，其相对于光学单元4设置有间隙并且固定在生物体A上，其中光学单元4包括用于将来自光源2的光转换成第一准直光学系统7 准直光L1并将其引入观察头5和用于聚焦来自观察头5的返回光以在图像获取装置3处形成图像的成像光学系统9，并且其中观察光学头5包括第二 用于聚焦从光学单元4入射的准直光L1以照射生物体A中的检查对象部位B的准直器光学系统15，以及将来自活体的返回光转换为准直光L2并将其引入光学 单位4。
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公开(公告)号：US07339148B2
公开(公告)日：2008-03-04
申请号：US10682236
申请日：2003-10-09
申请人： Yoshihiro Kawano , Kiyoshi Koike
发明人： Yoshihiro Kawano , Kiyoshi Koike
CPC分类号： G02B21/004 , G02B21/002 , G02B21/0032 , G02B21/0048
摘要： A scanning optical microscope including: a light source; a lens for altering the cross-sectional shape aspect ratio of a beam of light emitted from the light source; at least one lens for converging beams of light of different cross-sectional shape aspect ratio to create a linear light; a first light modulation member able to impart shade to the converged linear light; a lens that can form the light to which the shade has been imparted as a parallel light; a scanning member that can alter the angle of illumination; a lens for focusing the light to which the shade has been imparted; an objective lens for projecting the light to which the shading has been imparted to a sample body; and a lens for imaging the reflected light from the sample body or the light generated by the sample body on to a sensor.
摘要翻译： 一种扫描光学显微镜，包括：光源; 用于改变从光源发射的光束的横截面形状纵横比的透镜; 至少一个透镜，用于会聚具有不同横截面形状纵横比的光束以产生线性光; 第一光调制构件，能够对会聚的线状光赋予遮光; 可以形成作为平行光的阴影赋予的光的透镜; 可以改变照明角度的扫描构件; 用于聚焦已经施加阴影的光的透镜; 用于将已经施加了阴影的光投射到样本体的物镜; 以及用于将来自样本体的反射光或由样本体产生的光成像到传感器的透镜。
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公开(公告)号：US07304790B2
公开(公告)日：2007-12-04
申请号：US11090036
申请日：2005-03-28
IPC分类号： G02B21/00
CPC分类号： G02B21/002 , G02B21/245
摘要： A focusing method for an examination apparatus that can quickly and easily perform focusing for fluoroscopy is provided. The focusing method, for an examination apparatus that can observe fluorescence emitted from a specimen, includes a first step of irradiating the specimen with light via an objective lens to generate reflected light and fluorescence; a second step of performing focusing with respect to the surface of the specimen using the reflected light from the specimen; and a third step of performing focusing for the fluorescence based on the focal position of the specimen surface in the second step.
摘要翻译： 提供了一种用于能够快速且容易地执行用于荧光透视的聚焦的检查装置的聚焦方法。 对于可以观察从样本发出的荧光的检查装置的聚焦方法包括：通过物镜用光照射样本以产生反射光和荧光的第一步骤; 使用来自样本的反射光对样本的表面进行聚焦的第二步骤; 以及在第二步骤中基于样品表面的焦点位置进行荧光聚焦的第三步骤。
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公开(公告)号：US07239384B2
公开(公告)日：2007-07-03
申请号：US11209693
申请日：2005-08-24
申请人： Yoshihiro Kawano
发明人： Yoshihiro Kawano
IPC分类号： G01J3/30
CPC分类号： G01N21/6456 , G01J3/021 , G01J3/0229 , G01J3/10 , G01J3/1256 , G01J3/4406 , G01J2003/1221 , G01J2003/1286 , G01N21/6486 , G01N2021/6419 , G01N2021/6421 , G01N2021/6463
摘要： A laser-scanning fluoroscopy apparatus includes a laser light source of a plurality of wavelengths; a spectroscopic device for splitting laser beams according to wavelength; a focusing lens for focusing the split laser beams; a wavelength-selecting reflection device which includes a plurality of reflection sections disposed near the focal positions, spaced out at predetermined intervals in a split direction to reflect the laser beams with different wavelengths and a transmission section arranged adjacent to the reflection sections; a diffraction grating for combining the reflected laser beams; a scanning section for two-dimensionally scanning the combined laser beams; an objective optical system for focusing the scanned laser beams onto a tissue; and a photodetector for detecting fluorescence emitted from the tissue.
摘要翻译： 激光扫描透视设备包括多个波长的激光光源; 用于根据波长分割激光束的分光装置; 用于聚焦分束激光束的聚焦透镜; 波长选择反射装置，其包括设置在聚焦位置附近的多个反射部分，以分割方向以预定间隔隔开以反射具有不同波长的激光束;以及透射部分，布置成与反射部分相邻; 用于组合反射的激光束的衍射光栅; 用于二维扫描组合的激光束的扫描部分; 用于将扫描的激光束聚焦到组织上的物镜光学系统; 以及用于检测从组织发出的荧光的光电检测器。
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10.发明授权公开(公告)号：US07176428B2
公开(公告)日：2007-02-13
申请号：US11070191
申请日：2005-03-03
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译： 本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。 多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自 标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。
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