公开(公告)号：US08815600B2

公开(公告)日：2014-08-26

申请号：US12660885

申请日：2010-03-05

申请人： Weiqiang Liu ,  Ping Yang ,  Tao Wang ,  Zhuying Wang ,  Wenzhu Chen ,  Fang Liang Zhang

发明人： Weiqiang Liu ,  Ping Yang ,  Tao Wang ,  Zhuying Wang ,  Wenzhu Chen ,  Fang Liang Zhang

IPC分类号： C12N15/64 ,  C12N15/70

CPC分类号： C12N15/66

摘要： Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.

摘要翻译： 描述了在预定位置将供体DNA分子克隆到受体载体中的方法和组合物。 该方法基于通过体外处理供体DNA和受体载体介导的同源重组，其中含有外切核酸酶和单链DNA结合蛋白的酶混合物。

公开(公告)号：US20130017574A1

公开(公告)日：2013-01-17

申请号：US13550120

申请日：2012-07-16

申请人： Zhuying WANG ,  Xiaowu LIU

发明人： Zhuying WANG ,  Xiaowu LIU

IPC分类号： C12P21/00 ,  C12P21/08 ,  C12N5/10 ,  C12N1/21 ,  C12N1/19 ,  C12N15/63 ,  C12N15/11

CPC分类号： C12N15/67 ,  C07K16/32 ,  C07K2317/10 ,  C07K2319/02 ,  C12P21/02

摘要： Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described.

摘要翻译： 描述了用于增强来自细胞的蛋白质的表达和分泌的优化的信号肽编码序列以及相关的组合物和方法。 优化的信号肽编码序列编码在起始密码子的紧下游的至少一个发夹结构的mRNA。 还描述了使用优化的信号肽编码序列获得优化的信号肽编码序列的方法和用于增强蛋白质表达和分泌的方法。

公开(公告)号：US20120129239A1

公开(公告)日：2012-05-24

申请号：US13362382

申请日：2012-01-31

申请人： Weiqiang LIU ,  Ping YANG ,  Tao WANG ,  Zhuying WANG ,  Wenzhu CHEN ,  Fang Liang ZHANG

发明人： Weiqiang LIU ,  Ping YANG ,  Tao WANG ,  Zhuying WANG ,  Wenzhu CHEN ,  Fang Liang ZHANG

IPC分类号： C12N9/16

CPC分类号： C12N15/66

摘要： Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.

摘要翻译： 描述了在预定位置将供体DNA分子克隆到受体载体中的方法和组合物。 该方法基于通过体外处理供体DNA和受体载体介导的同源重组，其中含有外切核酸酶和单链DNA结合蛋白的酶混合物。

公开(公告)号：US20100326828A1

公开(公告)日：2010-12-30

申请号：US12797011

申请日：2010-06-09

申请人： Zhuying WANG ,  Jinyu YANG ,  Fanli MENG ,  Bing LAI ,  Zhan DAI ,  Fang Liang ZHANG

发明人： Zhuying WANG ,  Jinyu YANG ,  Fanli MENG ,  Bing LAI ,  Zhan DAI ,  Fang Liang ZHANG

IPC分类号： G01N27/26

CPC分类号： G01N27/44726

摘要： An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described.

摘要翻译： 描述了一种改进的染色方法，用于染色基质中生物聚合物如肽，蛋白质，RNA，DNA，寡糖或含有肽，蛋白质，RNA，DNA或寡糖的复合物。 该方法包括使用电力将染色剂移动到基质中的步骤。 相对于常规技术，染色时间可以显着降低。 改进的染色方法可以特别用于例如在凝胶分离后染色蛋白质。 还描述了其它相关方法和相关试剂盒。

公开(公告)号：US08968541B2

公开(公告)日：2015-03-03

申请号：US12797011

申请日：2010-06-09

申请人： Zhuying Wang ,  Jinyu Yang ,  Fanli Meng ,  Bing Lai ,  Zhan Dai ,  Fang Liang Zhang

发明人： Zhuying Wang ,  Jinyu Yang ,  Fanli Meng ,  Bing Lai ,  Zhan Dai ,  Fang Liang Zhang

IPC分类号： B01D57/02 ,  G01N33/559 ,  G01N27/447

CPC分类号： G01N27/44726

摘要： An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described.

摘要翻译： 描述了一种改进的染色方法，用于染色基质中生物聚合物如肽，蛋白质，RNA，DNA，寡糖或含有肽，蛋白质，RNA，DNA或寡糖的复合物。 该方法包括使用电力将染色剂移动到基质中的步骤。 相对于常规技术，染色时间可以显着降低。 改进的染色方法可以特别用于例如在凝胶分离后染色蛋白质。 还描述了其它相关方法和相关试剂盒。

公开(公告)号：US08326547B2

公开(公告)日：2012-12-04

申请号：US12894401

申请日：2010-09-30

申请人： Xiaowu Liu ,  Yun He ,  Zhuying Wang ,  Chunjiao Wang ,  Zhibing Liu ,  Tianhui Xia ,  Luquan Wang ,  Fang Liang Zhang

发明人： Xiaowu Liu ,  Yun He ,  Zhuying Wang ,  Chunjiao Wang ,  Zhibing Liu ,  Tianhui Xia ,  Luquan Wang ,  Fang Liang Zhang

IPC分类号： G06F19/10 ,  G06F19/00

CPC分类号： G06F19/22 ,  G06F19/20

摘要： An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

摘要翻译： 描述了改进的基因序列优化方法，该系统优化方法用于增强细菌，酵母，昆虫和哺乳动物细胞中基因的重组表达。 这种一般方法考虑了影响蛋白质表达的参数和因子的多个，优选大多数或全部，包括密码子使用，tRNA使用，GC含量，核糖体结合序列，启动子，5'-UTR，ORF和3'-UTR 改进和优化基因序列以增强细菌，酵母，昆虫和哺乳动物细胞中基因的蛋白质表达的基因序列。 特别地，本发明涉及使用粒子群优化算法改进重组蛋白表达的序列优化的系统和方法。 改进的系统优化方法可以被并入软件中以进行更有效的优化。

公开(公告)号：US20110008807A1

公开(公告)日：2011-01-13

申请号：US12885837

申请日：2010-09-20

申请人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

发明人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

IPC分类号： G01N33/543 ,  G01N33/566

CPC分类号： G01N33/54393

摘要： The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

摘要翻译： 本发明提供了一种抗原例如化合物，肽，核酸或原位从细胞或病毒颗粒释放的蛋白质的改进和快速检测方法。 相对于常规技术，抗原的检测时间可以显着降低。 该技术可以特别用于例如在传统ELISA中显着修饰和减少检测时间，以及Western印迹或斑点印迹测定。 改进的ELISA方法快速，经济，可重复，简单，可自动化。 还提供了使用改进的ELISA方法快速检测抗原的组合物和试剂盒。

公开(公告)号：US07816092B2

公开(公告)日：2010-10-19

申请号：US12626327

申请日：2009-11-25

申请人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

发明人： Zhuying Wang ,  Fang Liang Zhang ,  Tao Bai

IPC分类号： G01N33/53

CPC分类号： G01N33/54393

摘要： The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

摘要翻译： 本发明提供了一种抗原例如化合物，肽，核酸或原位从细胞或病毒颗粒释放的蛋白质的改进和快速检测方法。 相对于常规技术，抗原的检测时间可以显着降低。 该技术可以特别用于例如在传统ELISA中显着修饰和减少检测时间，以及Western印迹或斑点印迹测定。 改进的ELISA方法快速，经济，可重复，简单，可自动化。 还提供了使用改进的ELISA方法快速检测抗原的组合物和试剂盒。

公开(公告)号：US20080153715A1

公开(公告)日：2008-06-26

申请号：US11861933

申请日：2007-09-26

申请人： Michael G. PAPPAS ,  Zhuying WANG

发明人： Michael G. PAPPAS ,  Zhuying WANG

IPC分类号： C40B60/10

CPC分类号： C12Q1/6827 ,  C12Q2565/519 ,  C12Q2565/137 ,  C12Q2563/149

摘要： A method and means of identifying nucleic acid oligomers is disclosed. A sample is split into parts and the parts are flowed through chromatography columns containing nucleic acid oligomer probes bound to a binding medium. Analyte oligomers are transiently hybridized to complementary probe oligomers during chromatography. Detection and analysis of oligomer peaks is used to identify the oligomers contained in the sample.

摘要翻译： 公开了鉴定核酸寡聚体的方法和方法。 将样品分成部分，并将部分流经色谱柱，其中含有与结合介质结合的核酸低聚物探针。 分析物低聚物在色谱过程中与互补探针低聚物瞬时杂交。 寡聚物峰的检测和分析用于鉴定样品中所含的低聚物。

公开(公告)号：US08603780B2

公开(公告)日：2013-12-10

申请号：US13550120

申请日：2012-07-16

申请人： Zhuying Wang ,  Xiaowu Liu

发明人： Zhuying Wang ,  Xiaowu Liu

IPC分类号： A61K38/00

CPC分类号： C12N15/67 ,  C07K16/32 ,  C07K2317/10 ,  C07K2319/02 ,  C12P21/02

摘要： Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described.

摘要翻译： 描述了用于增强来自细胞的蛋白质的表达和分泌的优化的信号肽编码序列以及相关的组合物和方法。 优化的信号肽编码序列编码在起始密码子的紧下游的至少一个发夹结构的mRNA。 还描述了使用优化的信号肽编码序列获得优化的信号肽编码序列的方法和用于增强蛋白质表达和分泌的方法。