公开(公告)号：US10919928B2

公开(公告)日：2021-02-16

申请号：US16009533

申请日：2018-06-15

Applicant: AJINOMOTO CO., INC.

Inventor： Kunihiro Hirai ,  Satoshi Katayama ,  Naoko Hirose ,  Taisuke Ichimaru ,  Ken Yamashita ,  Daisuke Takahashi

IPC: C07H21/02 ,  C07H21/04 ,  C12N15/09 ,  C07H19/167 ,  C07H19/20 ,  C07H1/00 ,  C07H19/10 ,  C07H21/00 ,  C07H19/073 ,  C07H19/173 ,  C07H19/067

Abstract: Oligonucleotides may be produced by a process, including (1) condensing a nucleoside, nucleotide or oligonucleotide (b), and a nucleoside, nucleotide or oligonucleotide (a), or a substituted nucleotide or oligonucleotide (α) in a non-polar solvent to give a reaction solution containing a phosphite triester product (c); (3) oxidizing or sulfurizing the phosphite triester product (c) to give a reaction solution containing an oligonucleotide (d) wherein the 5′-hydroxy group is protected; (4) deprotecting the oligonucleotide (d) to give a reaction solution containing an oligonucleotide (e) wherein the 5′-hydroxy group is not protected; and (6) adding a polar solvent to the reaction solution containing the oligonucleotide (e) and purifying the oligonucleotide (e) by solid-liquid separation, wherein said nucleoside, nucleotide or oligonucleotide (a) or said substituted nucleotide or oligonucleotide (α) is a compound represented by formula (a-i): wherein Base, Rp1, R10, m, L, Y, and Z are defined herein.

公开(公告)号：US10464966B2

公开(公告)日：2019-11-05

申请号：US15655280

申请日：2017-07-20

Applicant: AJINOMOTO CO., INC.

Inventor： Kunihiro Hirai ,  Satoshi Katayama ,  Naoko Hirose ,  Ken Yamashita ,  Taisuke Ichimaru ,  Daisuke Takahashi

IPC: C07K1/32 ,  C07H21/02 ,  C07K1/30 ,  C07H21/04 ,  C07H21/00 ,  C07H23/00 ,  C07F7/18 ,  C07C43/10 ,  C07C43/205 ,  C07C49/84 ,  C07C69/157 ,  C07C69/78 ,  C07C233/47 ,  C07D295/108 ,  C07C69/017 ,  C07C69/24 ,  C07C69/757 ,  C07C69/92 ,  C07C69/94 ,  C07C43/23

Abstract: Precipitation promoters, which are an organic compound having one or more linear aliphatic hydrocarbon groups having not less than 10 carbon atoms, wherein the aliphatic hydrocarbon group has not less than 20 carbon atoms in total are useful for precipitating an organic compound protected by an organic group having one or more aliphatic hydrocarbon groups having not less than 10 carbon atoms from a solvent.

公开(公告)号：US20180282365A1

公开(公告)日：2018-10-04

申请号：US16009533

申请日：2018-06-15

Applicant: AJINOMOTO CO., INC.

Inventor： Kunihiro HIRAI ,  Satoshi Katayama ,  Naoko Hirose ,  Taisuke Ichimaru ,  Ken Yamashita ,  Daisuke Takahashi

IPC: C07H21/02

Abstract: Oligonucleotides may be produced by a process, including (1) condensing a nucleoside, nucleotide or oligonucleotide (a) or the like wherein a 5′-hydroxy group is not protected and a nucleoside, nucleotide or oligonucleotide (b) wherein a 5′-hydroxy group is protected in a non-polar solvent to give a reaction solution containing a phosphite triester product (c); (3) oxidizing or sulfurizing the phosphite triester product (c) to give a reaction solution containing an oligonucleotide (d) wherein the 5′-hydroxy group is protected; (4) deprotecting the oligonucleotide (d) to give a reaction solution containing an oligonucleotide (e) wherein the 5′-hydroxy group is not protected; and (6) adding a polar solvent to the reaction solution containing the oligonucleotide (e) and purifying the oligonucleotide (e) by solid-liquid separation or extraction.