公开(公告)号：US20120156766A1

公开(公告)日：2012-06-21

申请号：US13379696

申请日：2010-06-15

申请人： Akiko Shiratori ,  Yoshiaki Yazawa ,  Takeshi Fujita ,  Kunio Harada ,  Kenko Uchida

发明人： Akiko Shiratori ,  Yoshiaki Yazawa ,  Takeshi Fujita ,  Kunio Harada ,  Kenko Uchida

IPC分类号： C12M1/40 ,  G01N21/76

CPC分类号： G01N21/76 ,  G01N33/54373

摘要： Temperature of a sensor chip itself rises with the supply of power owing to temperature dependence of the sensor chip as its basic characteristics. When a chemiluminescence reagent is added at the point of time at which the temperature rise reaches a steady state and a sensor chip photodiode dark current becomes constant, a drastic shift occurs in the sensor chip temperature. Remarkable dispersion occurs at this time in the sensor chip photodiode dark current Variance (unstability) of the sensor chip photodiode dark current can be decreased by reducing the temperature fluctuation of the sensor chip to minimum by using an exothermal effect of a thermal diffusion medium.

摘要翻译： 由于传感器芯片的温度依赖性，传感器芯片本身的温度随着功率的供给而上升。 当在温度上升达到稳定的时间点和传感器芯片光电二极管暗电流恒定的时间点添加化学发光试剂时，传感器芯片温度发生急剧的偏移。 传感器芯片光电二极管此时发生明显的色散通过使用热扩散介质的放热效应，传感器芯片的光电二极管暗电流的差异（不稳定性）可以通过将传感器芯片的温度波动降至最低来降低。

公开(公告)号：US20070292897A1

公开(公告)日：2007-12-20

申请号：US11812156

申请日：2007-06-15

申请人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

发明人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

IPC分类号： G01N33/542 ,  G01N33/53 ,  C12M3/00

CPC分类号： G01N33/558 ,  G01N33/54386

摘要： There is provided a simple, inexpensive reaction analysis kit and system capable of performing highly sensitive, quantitative measurement. A chemical luminescence reaction is employed and a sensor element is used to detect the reaction in a highly sensitive manner. That is, a reaction detection plate is used to transmit a signal detected in the sensor element via a reader coil and a reader and then analyze the reaction. The reaction detection plate has a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is provided in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

摘要翻译： 提供了一种简单，便宜的反应分析试剂盒和系统，能够进行高灵敏度，定量测量。 采用化学发光反应，使用传感器元件以高度敏感的方式检测反应。 也就是说，反应检测板用于通过读取器线圈和读取器传输在传感器元件中检测到的信号，然后分析反应。 反应检测板具有a）膜，b）第一抗体浸渍部分，其被设置为使其面向膜并保持特异性结合待分析物质的第一标记抗体，c）第二抗体固定化部分，其中 提供在膜的一部分中，并且具有固定的第二抗体，特异性结合待分析物质的第二抗体，和d）设置为使得其面对第二抗体固定化部分并且包括光检测器的传感器元件， 一个信号收发器。

公开(公告)号：US07985384B2

公开(公告)日：2011-07-26

申请号：US11812156

申请日：2007-06-15

申请人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

发明人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

IPC分类号： G01N21/75 ,  G01N31/22 ,  G01N33/52

CPC分类号： G01N33/558 ,  G01N33/54386

摘要： There is provided a simple, inexpensive reaction analysis kit and system capable of performing highly sensitive, quantitative measurement. A chemical luminescence reaction is employed and a sensor element is used to detect the reaction in a highly sensitive manner. That is, a reaction detection plate is used to transmit a signal detected in the sensor element via a reader coil and a reader and then analyze the reaction. The reaction detection plate has a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is provided in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

摘要翻译： 提供了一种简单，便宜的反应分析试剂盒和系统，能够进行高灵敏度，定量测量。 采用化学发光反应，使用传感器元件以高度敏感的方式检测反应。 也就是说，反应检测板用于通过读取器线圈和读取器传输在传感器元件中检测到的信号，然后分析反应。 反应检测板具有a）膜，b）第一抗体浸渍部分，其被设置为使其面向膜并保持特异性结合待分析物质的第一标记抗体，c）第二抗体固定化部分，其中 提供在膜的一部分中并且具有固定的第二抗体，特异性结合待分析物质的第二抗体，以及d）设置成使得其面向第二抗体固定化部分并且包括光检测器的传感器元件， 一个信号收发器。

公开(公告)号：US09834806B2

公开(公告)日：2017-12-05

申请号：US13001465

申请日：2009-06-25

申请人： Hideyuki Noda ,  Masahiro Okanojo ,  Kenko Uchida ,  Norihito Kuno

发明人： Hideyuki Noda ,  Masahiro Okanojo ,  Kenko Uchida ,  Norihito Kuno

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12Q1/06 ,  C12Q1/00 ,  B01L3/00 ,  G01N1/22

CPC分类号： C12Q1/008 ,  B01L3/502 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/1805 ,  B01L2400/0487 ,  G01N1/2208 ,  G01N1/2273

摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped. The contact with the warm water causes the carrier to be solated filtered through the filter.

公开(公告)号：US08040515B2

公开(公告)日：2011-10-18

申请号：US12051245

申请日：2008-03-19

申请人： Tsuyoshi Sonehara ,  Takashi Anazawa ,  Kenko Uchida ,  Tomoyuki Sakai

发明人： Tsuyoshi Sonehara ,  Takashi Anazawa ,  Kenko Uchida ,  Tomoyuki Sakai

IPC分类号： G01N21/25

CPC分类号： G01N21/6452 ,  G02B2207/113

摘要： In order to provide a fluorescence detection apparatus having a high sensitivity, a high processing capacity and a competitive edge in cost, the fluorescence detection apparatus according to this invention irradiate the sample with light so that the aspect ratio of the form of the irradiated region by light on the arrangement surface of the sample may be 1±0.1. The preferable form of irradiate region is not limited to one and varies to some extent depending on the item to be optimized. The form of irradiated region may be, for example, a circle, an equilateral triangle, a square, a regular hexagon and the like.

摘要翻译： 为了提供具有高灵敏度，高处理能力和成本方面的竞争优势的荧光检测装置，根据本发明的荧光检测装置用光照射样品，使得照射区域的形式的纵横比由 样品排列表面上的光可能为1±0.1。 照射区域的优选形式不限于一个，并且根据要优化的项目而在一定程度上变化。 照射区域的形式可以是例如圆形，等边三角形，正方形，正六边形等。

公开(公告)号：US07932486B2

公开(公告)日：2011-04-26

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/26

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US07798981B2

公开(公告)日：2010-09-21

申请号：US12267056

申请日：2008-11-07

申请人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Kenko Uchida ,  Hideo Kawaguchi ,  Minoru Sakairi

发明人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Kenko Uchida ,  Hideo Kawaguchi ,  Minoru Sakairi

IPC分类号： A61B5/103 ,  A61B5/05

CPC分类号： A61B5/1101 ,  A61B5/4082 ,  A61B5/6824 ,  A61B5/6826 ,  A61B5/6828 ,  A61B5/6838 ,  A61B5/7239

摘要： A living body inspection apparatus including an oscillation coil which passes an AC current, a detection coil which detects an AC magnetic field generated from the detection coil, an amplification circuit which amplifies a voltage generated by the magnetic field induced by the detection coil, a detecting unit for detecting the output signal of the amplification circuit, a low pass filter to which the output signal of the detecting unit is input, a unit for setting the oscillation coil and detection coil in first and second regions of the living body, a recording unit for recording the output of the low pass filter while the first region and the second region of the living body are moving and a displaying unit for displaying the data recorded in the recording unit or results of analysis of the recorded data.

摘要翻译： 一种生物体检查装置，包括通过交流电流的振荡线圈，检测由检测线圈产生的交流磁场的检测线圈，放大电路，放大电路，放大由检测线圈感应出的磁场产生的电压， 用于检测放大电路的输出信号的单元，输入检测单元的输出信号的低通滤波器，用于在生物体的第一和第二区域设置振荡线圈和检测线圈的单元，记录单元 用于在活体的第一区域和第二区域移动时记录低通滤波器的输出，以及用于显示记录在记录单元中的数据的显示单元或记录数据的分析结果。

公开(公告)号：US07231241B2

公开(公告)日：2007-06-12

申请号：US11070816

申请日：2005-03-03

申请人： Yukiko Hirabayashi ,  Kenko Uchida

发明人： Yukiko Hirabayashi ,  Kenko Uchida

IPC分类号： A61B5/00

CPC分类号： A61B5/6814 ,  A61B5/0042 ,  A61B5/0261 ,  A61B5/14553 ,  A61B2562/0233 ,  A61B2562/046

摘要： Disclosed is a probe for biomeasurement by use of light capable of adjusting positions of incident points and detection points without changing the distance between the incident point and the detection point in accordance with the size of a subject's head, and an optical bioinstrumentation for living body using the probe. The distances between the incident points and the detection points are approximately the same. A gap is formed by removing part of connecting members, rotatable about the incident point and the detection point, around the subject head top portion. The size of the probe is changed by changing the distance between the incident points or the detection points around the gap.

摘要翻译： 公开了一种用于通过使用能够调整入射点和检测点的位置的光而不改变入射点与检测点之间的距离的光的探针，该探针根据受检者的头部的尺寸和使用的生物体的光学生物仪器 探针。 入射点和检测点之间的距离大致相同。 通过将围绕入射点和检测点旋转的连接构件的一部分围绕被检体顶部部分去除而形成间隙。 通过改变入射点或间隙周围的检测点之间的距离来改变探头的尺寸。

公开(公告)号：US08680483B2

公开(公告)日：2014-03-25

申请号：US13141127

申请日：2009-12-22

申请人： Takanobu Haga ,  Tsuyoshi Sonehara ,  Kenko Uchida ,  Tomoyuki Sakai ,  Satoshi Takahashi

发明人： Takanobu Haga ,  Tsuyoshi Sonehara ,  Kenko Uchida ,  Tomoyuki Sakai ,  Satoshi Takahashi

IPC分类号： G01J1/58

CPC分类号： G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6478

摘要： A fluorescence detector in which a sample substrate is provided with a structure unit comprising a prism or a diffraction grating. After excitation light falling on the sample substrate is totally reflected at a biomolecule-immobilized face that is located in the opposite side of the structure unit, the structure unit allows the emission of the reflected light therefrom. To ensure multiple visual field measurement, a sample substrate-driving unit is provided to scan the sample substrate.

摘要翻译： 一种荧光检测器，其中样品基板设置有包括棱镜或衍射光栅的结构单元。 落在样品基板上的激发光在位于结构单元的相对侧的生物分子固定面全面反射，结构单元允许从其发射反射光。 为了确保多个视野测量，提供了样品基板驱动单元以扫描样品基板。

公开(公告)号：US20140065704A1

公开(公告)日：2014-03-06

申请号：US14111036

申请日：2012-04-10

申请人： Masataka Shirai ,  Hiroyuki Tsunoda ,  Hiroko Matsunaga ,  Kenko Uchida

发明人： Masataka Shirai ,  Hiroyuki Tsunoda ,  Hiroko Matsunaga ,  Kenko Uchida

IPC分类号： C12M1/26

CPC分类号： C12M33/04 ,  C12M47/04

摘要： The present invention provides a simple and highly reliable cell collection system with high throughput and improved in cell collection efficiency. In the present invention, one or more pores are formed in a cell collection plate. One surface of the plate can be directly introduced in e.g., a petri dish, so as to be in contact with a solution containing cells. In this case, means for obtaining an optical image of collected cells from its rear surface is provided to improve reliability and convenience during a cell collection process. Alternatively, the vicinities of the pores in the cell collection plate are only hydrophilized and the other region is made water repellent to improve the efficiency of cell collection.

摘要翻译： 本发明提供了一种具有高通量并提高了细胞收集效率的简单且高度可靠的细胞收集系统。 在本发明中，在细胞收集板中形成一个以上的孔。 板的一个表面可以直接引入例如陪替氏培养皿中，以便与含有细胞的溶液接触。 在这种情况下，提供用于从其后表面获得收集的细胞的光学图像的装置，以提高细胞收集过程中的可靠性和便利性。 或者，细胞收集板中的孔附近仅被亲水化，另一区域被制成防水剂，以提高细胞收集的效率。